دورية أكاديمية
A rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation.
| العنوان: | A rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation. |
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| المؤلفون: | Turiello R; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rat3a@virginia.edu., Nouwairi RL; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rat3a@virginia.edu., Keller J; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rat3a@virginia.edu., Cunha LL; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rat3a@virginia.edu., Dignan LM; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rat3a@virginia.edu., Landers JP; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. rat3a@virginia.edu.; Department of Mechanical Engineering, University of Virginia, Charlottesville, VA, USA.; Department of Pathology, University of Virginia, Charlottesville, VA, USA. |
| المصدر: | Lab on a chip [Lab Chip] 2023 Dec 20; Vol. 24 (1), pp. 97-112. Date of Electronic Publication: 2023 Dec 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101128948 Publication Model: Electronic Cited Medium: Internet ISSN: 1473-0189 (Electronic) Linking ISSN: 14730189 NLM ISO Abbreviation: Lab Chip Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : Royal Society of Chemistry, c2001- |
| مواضيع طبية MeSH: | Epigenomics* , DNA*/genetics, Humans ; Sequence Analysis, DNA/methods ; Epigenesis, Genetic |
| مستخلص: | The approaches to forensic human identification (HID) are largely comparative in nature, relying upon the comparison of short tandem repeat profiles to known reference materials and/or database profiles. However, many profiles are generated from evidence materials that either do not have a reference material for comparison or do not produce a database hit. As an alternative to individualizing analysis for HID, researchers of forensic DNA have demonstrated that the human epigenome can provide a wealth of information. However, epigenetic analysis requires sodium b̲is̲ulfite c̲onversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, we describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. |
| المشرفين على المادة: | TZX5469Z6I (sodium bisulfite) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20231129 Date Completed: 20231221 Latest Revision: 20240229 |
| رمز التحديث: | 20240229 |
| DOI: | 10.1039/d3lc00867c |
| PMID: | 38019115 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1473-0189 |
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| DOI: | 10.1039/d3lc00867c |