دورية أكاديمية
Use of Esienia fetida Worms to Produce Peptone for Clostridium perfringens Vaccine Production.
| العنوان: | Use of Esienia fetida Worms to Produce Peptone for Clostridium perfringens Vaccine Production. |
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| المؤلفون: | Noruzy Moghadam H; Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran., Hemmaty M; Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran., Farzin HR; Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran., Jamshidian Mojaver M; Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran., Jandaghi H; Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran., Majidi B; Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran. |
| المصدر: | Archives of Razi Institute [Arch Razi Inst] 2023 Jun 30; Vol. 78 (3), pp. 1041-1047. Date of Electronic Publication: 2023 Jun 30 (Print Publication: 2023). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Razi Vaccine and Serum Research Institute Country of Publication: Iran NLM ID: 101549567 Publication Model: eCollection Cited Medium: Internet ISSN: 2008-9872 (Electronic) Linking ISSN: 03653439 NLM ISO Abbreviation: Arch Razi Inst Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2006- : Karaj, Iran : Razi Vaccine and Serum Research Institute Original Publication: 1999-2005 : Tehran, Iran : Razi Vaccine and Serum Research Institute |
| مواضيع طبية MeSH: | Clostridium perfringens*/metabolism , Peptones*/metabolism, Humans ; Animals ; Mice ; Meat/microbiology ; Iran |
| مستخلص: | Concurrent with an increase in the human population on the earth, more than ever, the creation of energy and maintenance of health is necessary, and nowadays, various sources of energy supply are being developed. The general global view in this regard is to provide protein and energy from available and cheap sources. Iran is no exception to this general rule, only in the field of ensuring the health of livestock resources every year, about 10 tons of peptone is needed for producing clostridial vaccines. Vermicomposting worms ( Esienia fetida ) with high protein percentages and rapid reproductions are a suitable source for peptone production. Based on this, the vaccine strain of Clostridium perfringens type D cultivated in two different media contain peptone produced from worms and meat peptone. The growth rate, epsilon toxin (ETX), and alpha toxin (CPA) of Cl. perfringens have been compared in two media. The results showed that the growth rate of bacteria in the worm peptone medium in 48 h was 22% higher than that of the meat peptone. Additionally, the activity of alpha toxin (phospholipase C) was in worm peptone 15% higher than meat peptone during 80 min of measurement. Regarding epsilon toxin lethality, all three mice of the N-worm peptone group died, while all three mice of the meat peptone group survived even 72 h after injection. The average survival time of mice in the N-worm peptone group was 1700 min. Therefore, we suggest the worms' protein is more suitable than industrial meat in peptone production for vicinal propose. To eliminate the need for hydrolyzed protein in the production of vaccines in the future, we suggest an increase in the fields of employment and the development of fertilizer and worm farms in Iran. Competing Interests: The authors declare that they have no conflict of interest. |
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| فهرسة مساهمة: | Keywords: Epsilon toxin; Peptone; Phospholipase C; Clostridium perfringens; Esienia fetida worm |
| المشرفين على المادة: | 0 (Peptones) |
| تواريخ الأحداث: | Date Created: 20231129 Date Completed: 20231201 Latest Revision: 20240223 |
| رمز التحديث: | 20240224 |
| مُعرف محوري في PubMed: | PMC10657954 |
| DOI: | 10.22092/ARI.2023.361078.2627 |
| PMID: | 38028845 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2008-9872 |
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| DOI: | 10.22092/ARI.2023.361078.2627 |