دورية أكاديمية
Genetic evidence for the interaction between Bacillus anthracis -encoded phage receptors and their cognate phage-encoded receptor binding proteins.
| العنوان: | Genetic evidence for the interaction between Bacillus anthracis -encoded phage receptors and their cognate phage-encoded receptor binding proteins. |
|---|---|
| المؤلفون: | Forrest S; Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Ton S; Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Sholes SL; Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Harrison S; Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Plaut RD; Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States., Verratti K; Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Wittekind M; Olympic Protein Technologies, Seattle, WA, United States., Ettehadieh E; Olympic Protein Technologies, Seattle, WA, United States., Necciai B; Joint Program Executive Office for Chemical, Biological, Radiological and Nuclear Defense (JPEO-CBRND), Joint Project Lead for CBRND Enabling Biotechnologies, Frederick, MD, United States., Sozhamannan S; Joint Program Executive Office for Chemical, Biological, Radiological and Nuclear Defense (JPEO-CBRND), Joint Project Lead for CBRND Enabling Biotechnologies, Frederick, MD, United States.; Joint Research and Development, Inc., Stafford, VA, United States., Grady SL; Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2023 Oct 31; Vol. 14, pp. 1278791. Date of Electronic Publication: 2023 Oct 31 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Bacteriophages such as γ and AP50c have been shown to infect strains of Bacillus anthracis with high specificity, and this feature has been exploited in the development of bacterial detection assays. To better understand the emergence of phage resistance, and thus the potential failure of such assays, it is important to identify the host and phage receptors necessary for attachment and entry. Using genetic approaches, the bacterial receptors of AP50c and γ have been identified as sap and GamR, respectively. A second AP50c-like phage, Wip1, also appears to use sap as a receptor. In parallel with this work, the cognate phage-encoded receptor binding proteins (RBPs) have also been identified (Gp14 for γ, P28 for AP50c, and P23 for Wip1); however, the strength of evidence supporting these protein-protein interactions varies, necessitating additional investigation. Here, we present genetic evidence further supporting the interaction between sap and the RBPs of AP50c and Wip1 using fluorescently tagged proteins and a panel of B. anthracis mutants. These results showed that the deletion of the sap gene, as well as the deletion of csaB , whose encoded protein anchors sap to the bacterial S-layer, resulted in the loss of RBP binding. Binding could then be rescued by expressing these genes in trans . We also found that the RBP of the γ-like prophage λBa03 relied on csaB activity for binding, possibly by a different mechanism. RBP Competing Interests: ShS was employed by Joint Research and Development, Inc. MW and EE were employed by Olympic Protein Technologies. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Forrest, Ton, Sholes, Harrison, Plaut, Verratti, Wittekind, Ettehadieh, Necciai, Sozhamannan and Grady.) |
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| فهرسة مساهمة: | Keywords: Bacillus anthracis; S-layer; bacterial receptors; fluorescence detection; phage resistance; phages; receptor binding proteins |
| تواريخ الأحداث: | Date Created: 20231129 Latest Revision: 20231201 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10644760 |
| DOI: | 10.3389/fmicb.2023.1278791 |
| PMID: | 38029077 |
| قاعدة البيانات: | MEDLINE |
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