دورية أكاديمية
Irisin Prevents Cell Death in High Glucose via NLRP3 Inhibition.
| العنوان: | Irisin Prevents Cell Death in High Glucose via NLRP3 Inhibition. |
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| المؤلفون: | Wei H, Zhou J, Qiu X, Niu H, Zhang Z, Liu Y, Bai P, Xin C, Wen W, Lu X, Cao G, Hu T |
| المصدر: | Alternative therapies in health and medicine [Altern Ther Health Med] 2024 Sep; Vol. 30 (9), pp. 194-201. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: InnoVision Communications Country of Publication: United States NLM ID: 9502013 Publication Model: Print Cited Medium: Print ISSN: 1078-6791 (Print) Linking ISSN: 10786791 NLM ISO Abbreviation: Altern Ther Health Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Aliso Viejo, CA : InnoVision Communications, c1995- |
| مواضيع طبية MeSH: | NLR Family, Pyrin Domain-Containing 3 Protein*/metabolism , Pyroptosis*/drug effects , Glucose*/metabolism , Fibronectins*/metabolism , Fibronectins*/pharmacology, Animals ; Rats ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Apoptosis/drug effects ; Cells, Cultured ; Humans ; Cell Death/drug effects |
| مستخلص: | Background: Impaired cardiac microvascular function has been implied in the pathophysiology of diabetic cardiovascular disease. However, the specific mechanism remains to be determined. Pyroptosis is a type of cell death that differs from apoptosis and autophagy. It is caused by the formation of plasma membrane pores through amino-terminal fragments of Gasdermin D (GSDMD), leading to the secretion of IL-1β and IL-18. Recent studies have shown that irisin, a myokine cleaved by the extracellular domain of FNDC5, plays a protective role in cardiovascular diseases. Here, we investigated the potential role of pyroptosis on the cardiac microvascular endothelial cells (CMECs) injury induced by high glucose (HG) and further determined the protective effect of irisin on pyroptosis. Methods: CMECs were cultured with normal glucose (control group, 5.5 mM) and high glucose (25 mM) medium for 12, 24, and 48 h respectively. The pyroptosis of CMECs was measured by immunofluorescence staining, ELISA, and Western blot assays. Moreover, the apoptosis level was determined by flow cytometry and TUNEL staining. Results: Our results showed that HG promoted apoptosis and pyroptosis. However, irisin reversed the increased apoptosis and pyroptosis. To investigate the underlying mechanism, we overexpressed the NLRP3 protein. We found the protective effect of irisin on apoptosis and pyroptosis was abolished by NLRP3 over-expression. Conclusions: Our data suggest that irisin protects CMECs against apoptosis and pyroptosis, at least in part, by inhibiting NLRP3 inflammasome. |
| المشرفين على المادة: | 0 (NLR Family, Pyrin Domain-Containing 3 Protein) IY9XDZ35W2 (Glucose) 0 (Fibronectins) 0 (FNDC5 protein, rat) |
| تواريخ الأحداث: | Date Created: 20231208 Date Completed: 20240926 Latest Revision: 20240926 |
| رمز التحديث: | 20240927 |
| PMID: | 38064628 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1078-6791 |
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