دورية أكاديمية
Adaptive cell wall thickening in Enterococcus faecalis is associated with decreased vancomycin susceptibility.
| العنوان: | Adaptive cell wall thickening in Enterococcus faecalis is associated with decreased vancomycin susceptibility. |
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| المؤلفون: | Wagner TM; Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway., Pöntinen AK; Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway; Department of Biostatistics, Faculty of Medicine, University of Oslo, Oslo, Norway., Al Rubaye M; Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway., Sundsfjord A; Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway; Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway., Hegstad K; Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway; Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway. Electronic address: kristin.hegstad@uit.no. |
| المصدر: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2024 Mar; Vol. 30 (3), pp. 396.e1-396.e5. Date of Electronic Publication: 2023 Dec 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 9516420 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-0691 (Electronic) Linking ISSN: 1198743X NLM ISO Abbreviation: Clin Microbiol Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Elsevier Original Publication: Paris : Decker Europe, c1995- |
| مواضيع طبية MeSH: | Gram-Positive Bacterial Infections*/microbiology , Enterococcus faecium*/genetics, Humans ; Vancomycin/pharmacology ; Enterococcus faecalis/genetics ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests ; Cell Wall |
| مستخلص: | Objectives: Enterococcus faecalis can adopt both a commensal and a nosocomial lifestyle, resisting numerous antibiotics. In this study, we aim to investigate the relationship between the cell wall (CW) thickness and decreased susceptibility to vancomycin (VD) in van-gene negative clinical isolates of E. faecalis (n Methods: The CW thickness was assessed in VD strains and compared with vancomycin susceptible isolates of the same sequence type (ST) (Vancomycin susceptible [VS]; n Results: The VD strains exhibited increased CW thickness when compared with ST-related VS strains (ΔCW thickness VD vs. VS ST30 25 nm, ST59 15 nm, and ST40 1 nm). Serial passages without vancomycin selection led to a decrease in CW thickness and vancomycin MIC in VD strains (ΔCW thickness VD vs. evVD ST30 22 nm, ST59 3 nm, and ST40 2 nm). Serial passages with vancomycin selection caused an increase in CW thickness and vancomycin MIC in ST-related VS strains (ΔCW thickness VS vs. evVS ST30 22 nm, ST59 16 nm, and ST40 1 nm). Discussion: Adaptive changes in CW thickness were observed in response to vancomycin exposure. Increased CW thickness correlated with decreased vancomycin susceptibility, whereas decreased CW thickness correlated with increased vancomycin susceptibility. Core single nucleotide polymorphisms in the evolved mutants were mostly found in genes encoding proteins associated with the cytoplasm or the cytoplasmic membrane. The potential relevance of these adaptive changes is underlined by the observed phenotypes in clinical isolates. Our findings emphasize the importance of monitoring adaptive changes, as vancomycin-resistant enterococci infections are a growing concern. (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Adaption; Cellular structures; Electron microscopy; Enterococci; Glycopeptides |
| المشرفين على المادة: | 6Q205EH1VU (Vancomycin) 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20231208 Date Completed: 20240220 Latest Revision: 20240220 |
| رمز التحديث: | 20240220 |
| DOI: | 10.1016/j.cmi.2023.12.002 |
| PMID: | 38065364 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1469-0691 |
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| DOI: | 10.1016/j.cmi.2023.12.002 |