دورية أكاديمية
Hyaluronic acid enhances cell migration, viability, and mineralized tissue-specific genes in cementoblasts.
| العنوان: | Hyaluronic acid enhances cell migration, viability, and mineralized tissue-specific genes in cementoblasts. |
|---|---|
| المؤلفون: | Hakki SS; Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkey., Bozkurt SB; Department of Biochemistry, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde, Turkey., Sculean A; Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland., Božić D; Department of Periodontology, School of Dental Medicine, University Clinical hospital, Zagreb, Croatia. |
| المصدر: | Journal of periodontal research [J Periodontal Res] 2024 Feb; Vol. 59 (1), pp. 63-73. Date of Electronic Publication: 2023 Dec 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 0055107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0765 (Electronic) Linking ISSN: 00223484 NLM ISO Abbreviation: J Periodontal Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Malden, MA : Wiley-Blackwell Original Publication: Copenhagen : Munksgaard International Publishers |
| مواضيع طبية MeSH: | Dental Cementum* , beta Catenin*/metabolism, Core Binding Factor Alpha 1 Subunit/metabolism ; Hyaluronic Acid/pharmacology ; Cell Line ; Osteocalcin/metabolism ; Integrin-Binding Sialoprotein/metabolism ; Cell Differentiation ; Cell Movement ; RNA, Messenger/metabolism |
| مستخلص: | Background/objectives: It has been repeatedly demonstrated that cementum formation is a crucial step in periodontal regeneration. Hyaluronic acid (HA) is an important component of the extracellular matrix which regulates cells functions and cell-cell communication. Hyaluronic acid/derivatives have been used in regenerative periodontal therapy, but the cellular effects of HA are still unknown. To investigate the effects of HA on cementoblast functions, cell viability, migration, mineralization, differentiation, and mineralized tissue-associated genes and cementoblast-specific markers of the cementoblasts were tested. Materials and Methods: Cementoblasts (OCCM-30) were treated with various dilutions (0, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128) of HA and examined for cell viability, migration, mineralization, and gene expressions. The mRNA expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), bone sialoprotein (BSP), collagen type I (COL-I), alkaline phosphatase (ALP), cementum protein-1 (CEMP-1), cementum attachment protein (CAP), and small mothers against decapentaplegic (Smad) -1, 2, 3, 6, 7, β-catenin (Ctnnb1) were performed with real-time polymerase chain reaction (RT-PCR). Total RNA was isolated on days 3 and 8, and cell viability was determined using MTT assay on days 1 and 3. The cell mineralization was evaluated by von Kossa staining on day 8. Cell migration was assessed 2, 4, 6, and 24 hours following exposure to HA dilutions using an in vitro wound healing assay (0, 1:2, 1:4, 1:8). Results: At dilution of 1:2 to 1:128, HA importantly increased cell viability (p < .01). HA at a dilution of 1/2 increased wound healing rates after 4 h compared to the other dilutions and the untreated control group. Increased numbers of mineralized nodules were determined at dilutions of 1:2, 1:4, and 1:8 compared with control group. mRNA expressions of mineralized tissue marker including COL-I, BSP, RunX2, ALP, and OCN significantly improved by HA treatments compared with control group both on 3 days and on 8 days (p < .01). Smad 2, Smad 3, Smad 7, and β-catenin (Ctnnb1) mRNAs were up-regulated, while Smad1 and Smad 6 were not affected by HA administration. Additionally, HA at dilutions of 1:2, 1:4, and 1:8 remarkably enhanced CEMP-1 and CAP expressions in a dilution- and time-dependent manner (p < .01). Conclusions: The present results have demonstrated that HA affected the expression of both mineralized tissue markers and cementoblast-specific genes. Positive effects of HA on the cementoblast functions demonstrated that HA application may play a key role in cementum regeneration. (© 2023 The Authors. Journal of Periodontal Research published by John Wiley & Sons Ltd.) |
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| فهرسة مساهمة: | Keywords: cell viability; cementoblast; hyaluronic acid; mRNA expression; migration; mineralization |
| المشرفين على المادة: | 0 (beta Catenin) 0 (Core Binding Factor Alpha 1 Subunit) 9004-61-9 (Hyaluronic Acid) 104982-03-8 (Osteocalcin) 0 (Integrin-Binding Sialoprotein) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20231209 Date Completed: 20240214 Latest Revision: 20240214 |
| رمز التحديث: | 20240214 |
| DOI: | 10.1111/jre.13201 |
| PMID: | 38069670 |
| قاعدة البيانات: | MEDLINE |
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