دورية أكاديمية
An assessment of alterations to human sperm methylation patterns in coronavirus disease 2019 infected and healthy control males.
| العنوان: | An assessment of alterations to human sperm methylation patterns in coronavirus disease 2019 infected and healthy control males. |
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| المؤلفون: | Stirland I; Department of Cell Biology and Physiology, Brigham Young University, Provo, Utah., Soares MR; Department of Obstetrics and Gynecology, Ribeirao Preto Medical School University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Furtado CLM; Department of Obstetrics and Gynecology, Ribeirao Preto Medical School University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil; University of Fortaleza, Experimental Biology Center, Fortaleza, Ceara, Brazil., Dos Reis RM; Department of Obstetrics and Gynecology, Ribeirao Preto Medical School University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Aston KI; Department of Surgery, University of Utah School of Medicine, Salt Lake City, Utah., Smith RP; Department of Cell Biology and Physiology, Brigham Young University, Provo, Utah., Jenkins TG; Department of Cell Biology and Physiology, Brigham Young University, Provo, Utah; Department of Surgery, University of Utah School of Medicine, Salt Lake City, Utah. Electronic address: tim_jenkins@byu.edu. |
| المصدر: | F&S science [F S Sci] 2024 Feb; Vol. 5 (1), pp. 2-15. Date of Electronic Publication: 2023 Dec 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 101765857 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-335X (Electronic) Linking ISSN: 2666335X NLM ISO Abbreviation: F S Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [New York] : Elsevier Inc., [2020]- |
| مواضيع طبية MeSH: | COVID-19*/genetics , COVID-19*/metabolism, Humans ; Male ; Semen ; SARS-CoV-2/genetics ; Spermatozoa/metabolism ; DNA Methylation/genetics |
| مستخلص: | Objective: To determine whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection affects male reproductive health, considering the many potential factors that contribute to declines in male fertility on a semiglobal scale. Design: In total, 64 human semen samples-32 treatment and 32 control-were laboratory processed and bioinformatically analyzed to assess differences in DNA methylation patterns. Implementing multiple bioinformatic tools, the analyses conducted will elicit between-group differences with respect to epigenetic age, epigenetic instability, semiglobal, and regional methylation, in addition to methylation patterns as a function of time since infection. Setting: University hospital. Patients: The study cohort of 64 individuals was drawn from a larger population of 94 volunteer participants recruited at the Human Reproduction Center at the Clinical Hospital of the Ribeirao Preto Medical School-University of São Paulo between June 2021 and January 2022 as well as in accordance with the ethical guidelines established by the Declaration of Helsinki. Intervention: Exposure to SARS-CoV-2. Main Outcome Measure(s): Effects on male reproductive health were reported as differences in DNA methylation measured using an array. Mean β values at key regulatory loci for human spermatocytes were analyzed and compared between groups. Further analysis of β values using epigenetic age, instability, semiglobal, and regional methylation tools provided an analysis with substantial breadth and depth. Results: In all analyses, there were no differences between groups. Considering these results, it can be inferred that infection with SARS-CoV-2 does not alter the epigenome of human spermatocytes in significant and/or persistent ways. Tangentially, these data also suggest that human male reproductive health is minimally altered by the virus, or that it is altered in a way that is independent of epigenetic programming. Conclusion: Infection with SARS-CoV-2 has been reportedly associated with alterations in male fertility. This study asserts that such alterations do not have an epigenetic basis but are likely a result of concomitant symptomatology, i.e., fever and inflammation. Across the multiple bioinformatic analyses conducted, the results of this test did not detect any differences in DNA methylation patterns between coronavirus disease 2019 and noncoronavirus disease semen donor groups. Competing Interests: Declaration of interests I.S. has nothing to disclose. M.R.S. reports funding from Instituto Nacional de Ciência e Tecnologia – Hormônio e Saúde da Mulher (CNPq), number 176841/2022-9 Clinical data and human semen samples to the research for the submitted work. C.F. has nothing to disclose. R.M.D.R. reports funding from Instituto Nacional de Ciência e Tecnologia – Hormônio e Saúde da Mulher (CNPq), number 176841/2022-9 Clinical data and human semen samples to the research for the submitted work. K.I.A. reports patents for Methods of identifying male fertility status and embryo quality and Systems and methods for determining the impact of age-related changes in sperm epigenome on offspring phenotype outside the submitted work. R.P.S. has nothing to disclose. T.G.J. has nothing to disclose. (Copyright © 2023 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: COVID-19; Fertility; SARS-CoV-2; methylation |
| تواريخ الأحداث: | Date Created: 20231209 Date Completed: 20240216 Latest Revision: 20240216 |
| رمز التحديث: | 20240216 |
| DOI: | 10.1016/j.xfss.2023.12.003 |
| PMID: | 38070681 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2666-335X |
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| DOI: | 10.1016/j.xfss.2023.12.003 |