دورية أكاديمية
Multi-functionalization of reduced graphene oxide nanosheets for tumor theragnosis: Synthesis, characterization, enzyme assay, in-silico study, radiolabeling and in vivo targeting evaluation.
العنوان: | Multi-functionalization of reduced graphene oxide nanosheets for tumor theragnosis: Synthesis, characterization, enzyme assay, in-silico study, radiolabeling and in vivo targeting evaluation. |
---|---|
المؤلفون: | Sakr TM; Radioisotopes Production Facility, Second Egyptian Research Reactor Complex, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt.; Radioactive Isotopes and Generator Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt., Elsabagh MF; Radioisotopes Production Facility, Second Egyptian Research Reactor Complex, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt. mohamed.elsabagh@eaea.sci.eg.; Labeled Compounds Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt. mohamed.elsabagh@eaea.sci.eg., Fayez H; Labeled Compounds Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt., Sarhan MO; Labeled Compounds Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt., Syam YM; Department of Therapeutic Chemistry/ National Research Centre, Cairo, Egypt., Anwar MM; Department of Therapeutic Chemistry/ National Research Centre, Cairo, Egypt., Motaleb MA; Labeled Compounds Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt., Zaghary WA; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, Egypt. |
المصدر: | Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences [Daru] 2024 Jun; Vol. 32 (1), pp. 77-95. Date of Electronic Publication: 2023 Dec 11. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 101125969 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2008-2231 (Electronic) Linking ISSN: 15608115 NLM ISO Abbreviation: Daru Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2018- : Cham : Springer International Publishing Original Publication: Tehran, Iran : The Faculty |
مواضيع طبية MeSH: | Graphite*/chemistry, Humans ; Animals ; Cell Line, Tumor ; Mice ; Female ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/chemistry ; Molecular Docking Simulation ; Poly (ADP-Ribose) Polymerase-1/antagonists & inhibitors ; Poly (ADP-Ribose) Polymerase-1/metabolism ; Nanostructures/chemistry ; Poly(ADP-ribose) Polymerase Inhibitors/pharmacology ; Poly(ADP-ribose) Polymerase Inhibitors/chemistry ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Tissue Distribution |
مستخلص: | Background: In this study, a combination of nanotechnology, organic synthesis and radiochemistry were utilized in order to design an efficient nano-system conjugated with a suitable radionuclide and an antitumor agent for possible application as tumor theragnostic agent. Method: Four novel compounds (3 and 4a-c) bearing tetrahydroquinazoline-7-sulfonohydrazide or 1,2,3,4-tetrahydroquinazoline-7-sulfonamide scaffold were designed. Then, docking study predicted that the compounds can be considered as potential inhibitors for PARP-1. Following that; the four compounds were synthesized and properly characterized using 1 HNMR, 13 CNMR, IR and Mass spectroscopy. The cytotoxic effect of the four compounds was evaluated against breast cancer cell line (MDA-MB-436), where compound 3 showed the most promising cytotoxic effect. The inhibitory effect of the four compounds was evaluated in vitro against PARP-1. Result: Carboxylated graphene oxide nanosheets (NGO-COOH) were synthesized by a modified Hummer's method and has size of range 40 nm. The NGO-COOH nanosheets were proven to be safe and biocompatible when tested in vitro against normal human lung fibroblast cells (MRC-5). The prepared NGO-COOH nanosheets were conjugated with compound 3 then radiolabeled with 99m Tc to yield 99m Tc-NGO-COOH-3 with a radiochemical yield of 98.5.0 ± 0.5%. 99m Tc-NGO-COOH-3 was injected intravenously in solid tumor bearing mice to study the degree of localization of the nano-system at tumor tissue. The results of the study revealed, excellent localization and retention of the designed nano-system at tumor tissues with targeting ratio of 9.0. Conclusion: Stirred a new candidate tumor theragnostic agent that is safe, selective and stable. (© 2023. The Author(s).) |
References: | Mol Cancer Ther. 2007 Mar;6(3):945-56. (PMID: 17363489) Acta Pol Pharm. 2013 Sep-Oct;70(5):833-49. (PMID: 24147361) Science. 2008 Feb 1;319(5863):627-30. (PMID: 18239128) Nat Rev Cancer. 2002 Jan;2(1):48-58. (PMID: 11902585) J Med Chem. 2001 Sep 13;44(19):3175-86. (PMID: 11543687) Nucl Med Biol. 2014 Mar;41(3):282-9. (PMID: 24461626) Biomaterials. 2012 Jun;33(16):4147-56. (PMID: 22386918) J Pharm Sci. 2012 Jun;101(6):2194-203. (PMID: 22415405) Biomaterials. 2013 Jan;34(4):1146-54. (PMID: 23171545) Bioorg Chem. 2019 Jun;87:655-666. (PMID: 30952061) Small. 2009 Oct;5(20):2253-9. (PMID: 19582730) Science. 2010 May 21;328(5981):1031-5. (PMID: 20378772) J Pharm Biomed Anal. 2007 Sep 21;45(1):1-19. (PMID: 17689910) Biomaterials. 2008 Mar;29(9):1207-15. (PMID: 18076987) J Med Chem. 2009 Nov 26;52(22):7170-85. (PMID: 19873981) Nucl Med Biol. 2015 Feb;42(2):164-70. (PMID: 25311750) J Nanobiotechnology. 2012 Sep 24;10:39. (PMID: 23006133) J Med Chem. 2016 Nov 10;59(21):9575-9598. (PMID: 27416328) Appl Radiat Isot. 2016 Jun;112:13-9. (PMID: 26974487) Mater Sci Eng C Mater Biol Appl. 2014 Dec;45:196-204. (PMID: 25491820) Acta Pol Pharm. 2013 Jul-Aug;70(4):687-708. (PMID: 23923393) J Med Chem. 2016 Jan 14;59(1):335-57. (PMID: 26652717) Bioorg Med Chem Lett. 2014 Jan 15;24(2):624-9. (PMID: 24360999) Int J Pharm. 2013 Jun 5;449(1-2):10-7. (PMID: 23562750) Clin Cancer Res. 2008 Apr 1;14(7):1931-7. (PMID: 18381930) Int J Pharm. 2005 Jan 31;289(1-2):189-95. (PMID: 15652211) J Immunol Methods. 1983 Dec 16;65(1-2):55-63. (PMID: 6606682) Oncogene. 2008 Oct 6;27(45):5904-12. (PMID: 18836471) BMC Med. 2015 Mar 05;13:45. (PMID: 25857315) Bioorg Med Chem. 2006 Sep 1;14(17):6022-6. (PMID: 16730993) Nano Lett. 2008 Jun;8(6):1679-82. (PMID: 18498200) Small. 2009 Mar;5(5):544-57. (PMID: 19226595) J Cancer Res Clin Oncol. 2015 May;141(5):769-84. (PMID: 25005786) Daru. 2019 Jun;27(1):49-58. (PMID: 30706223) J Labelled Comp Radiopharm. 2017 Sep;60(11):502-520. (PMID: 28618064) Adv Drug Deliv Rev. 2008 Dec 14;60(15):1615-26. (PMID: 18840489) Small. 2010 Feb 22;6(4):537-44. (PMID: 20033930) Appl Radiat Isot. 2021 Dec;178:109970. (PMID: 34619628) ACS Chem Biol. 2013 Aug 16;8(8):1698-703. (PMID: 23742272) Front Chem. 2018 Jun 20;6:224. (PMID: 29974048) Int J Pharm. 2012 Jan 17;422(1-2):472-8. (PMID: 22101288) J Enzyme Inhib Med Chem. 2019 Dec;34(1):150-162. (PMID: 30427217) Cancer Treat Rev. 2011 Feb;37(1):63-74. (PMID: 20570444) Biomaterials. 2011 Jun;32(17):4151-60. (PMID: 21367450) Mater Sci Eng C Mater Biol Appl. 2019 May;98:1252-1276. (PMID: 30813007) FEBS J. 2013 Aug;280(15):3563-75. (PMID: 23601167) Exp Hematol. 2003 Jun;31(6):446-54. (PMID: 12829019) Int J Nanomedicine. 2012;7:953-64. (PMID: 22393292) J Colloid Interface Sci. 2014 Nov 1;433:163-175. (PMID: 25128864) Bioorg Med Chem. 2008 Jul 15;16(14):6965-75. (PMID: 18541433) J Nucl Med. 2000 Mar;41(3):463-9. (PMID: 10716320) Biochim Biophys Acta. 2014 Aug;1846(1):201-15. (PMID: 25026313) Biomaterials. 2015 Jan;39:234-48. (PMID: 25468374) Adv Mater. 2013 Apr 24;25(16):2258-68. (PMID: 23494834) Sci Rep. 2020 Feb 17;10(1):2717. (PMID: 32066812) Small. 2010 Jun 6;6(11):1205-9. (PMID: 20461727) Nat Biotechnol. 2007 Oct;25(10):1165-70. (PMID: 17891134) J Cell Sci. 2012 Dec 1;125(Pt 23):5591-6. (PMID: 23420197) |
فهرسة مساهمة: | Keywords: Active targeting; In-silico study; Nano synthesis; Nanographene sheets; PRAP-1 inhibitor; Radiolabeling; Technetium-99m; Tumor imaging; Tumor theragnosis |
المشرفين على المادة: | 7782-42-5 (Graphite) 0 (graphene oxide) 0 (Antineoplastic Agents) EC 2.4.2.30 (Poly (ADP-Ribose) Polymerase-1) 0 (Poly(ADP-ribose) Polymerase Inhibitors) EC 2.4.2.30 (PARP1 protein, human) |
تواريخ الأحداث: | Date Created: 20231210 Date Completed: 20240510 Latest Revision: 20240513 |
رمز التحديث: | 20240513 |
مُعرف محوري في PubMed: | PMC11087444 |
DOI: | 10.1007/s40199-023-00487-7 |
PMID: | 38072913 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2008-2231 |
---|---|
DOI: | 10.1007/s40199-023-00487-7 |