دورية أكاديمية
Use of multiplex PCR in pleural effusion: is it necessary to change the paradigm of culture-based methods?
| العنوان: | Use of multiplex PCR in pleural effusion: is it necessary to change the paradigm of culture-based methods? |
|---|---|
| المؤلفون: | Londono-Ruiz JP; Pediatrics Infectious Diseases, Clinica Infantil Colsubsidio, Bogotá, Colombia.; Pediatrics Infectious Diseases, Clinica Infantil Santa Maria del Lago, Bogotá, Colombia., Vargas-Garcia A; Pediatrics Infectious Diseases Fellow, Instituto Nacional de Pediatria, Ciudad de Mexico, Mexico., Cardenas-Muller A; Pediatric Intensive Care Unit, Clinica Infantil Colsubsidio, Bogotá, Colombia., Bejarano-Quintero AM; Pediatric Intensive Care Unit, Clinica Infantil Colsubsidio, Bogotá, Colombia., Mantilla MX; Microbiology Laboratory, Clinica Infantil Colsubsidio, Bogotá, Colombia., Gutierrez-Tobar IF; Pediatrics Infectious Diseases, Clinica Infantil Colsubsidio, Bogotá, Colombia.; Pediatrics Infectious Diseases, Clinica Infantil Santa Maria del Lago, Bogotá, Colombia. |
| المصدر: | Access microbiology [Access Microbiol] 2023 Nov 15; Vol. 5 (11). Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 101746927 Publication Model: eCollection Cited Medium: Internet ISSN: 2516-8290 (Electronic) Linking ISSN: 25168290 NLM ISO Abbreviation: Access Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Microbiology Society, [2019]- |
| مستخلص: | The microbiological diagnosis of pleural effusion is based largely on classical microbiology methods, but these methods have a high rate of false negative results. Some previous studies have shown improved diagnostic performance for pathogens such as Streptococcus pneumoniae using molecular biology methods. We present the use of a multiplex PCR platform (BIOFIRE FILMARRAY Pneumonia Panel) for the aetiological diagnosis of pleural effusion in paediatric pneumonia. We present a case series of 17 pleural fluid samples that were processed by culture-based microbiology and molecular biology methods. Microbiological isolation was successful in four cases (25 %) through traditional culture methods. In contrast, the molecular biology panels allowed for detection in 16 out of 17 cases (94 %). The results from these panels led to a change in management for nine out of the 17 cases (52 %). This study found an increase in aetiological diagnosis in complicated pneumonia in children by using molecular biology methods, which led to a significant change in patient management. Competing Interests: The authors declare that there are no conflicts of interest. (© 2023 The Authors.) |
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| فهرسة مساهمة: | Keywords: children; multiplex PCR; pleural effusion; pleural empyema; pneumonia |
| تواريخ الأحداث: | Date Created: 20231211 Latest Revision: 20231211 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10702381 |
| DOI: | 10.1099/acmi.0.000612.v3 |
| PMID: | 38074107 |
| قاعدة البيانات: | MEDLINE |
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