دورية أكاديمية
Purine and carbohydrate availability drive Enterococcus faecalis fitness during wound and urinary tract infections.
| العنوان: | Purine and carbohydrate availability drive Enterococcus faecalis fitness during wound and urinary tract infections. |
|---|---|
| المؤلفون: | Tan CAZ; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University Singapore, Singapore, Singapore., Chong KKL; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University Singapore, Singapore, Singapore., Yeong DYX; School of Biological Sciences, Nanyang Technological University Singapore, Singapore, Singapore., Ng CHM; School of Biological Sciences, Nanyang Technological University Singapore, Singapore, Singapore., Ismail MH; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University Singapore, Singapore, Singapore., Yap ZH; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University Singapore, Singapore, Singapore., Khetrapal V; Infectious Diseases Translational Research Programme, Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore., Tay VSY; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore., Drautz-Moses DI; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University Singapore, Singapore, Singapore., Ali Y; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.; Singapore Eye Research Institute (SERI), Singapore General Hospital, Singapore, Singapore., Chen SL; Infectious Diseases Translational Research Programme, Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Laboratory of Bacterial Genomics, Genome Institute of Singapore, Singapore, Singapore., Kline KA; Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University Singapore, Singapore, Singapore.; School of Biological Sciences, Nanyang Technological University Singapore, Singapore, Singapore.; Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland. |
| المصدر: | MBio [mBio] 2024 Jan 16; Vol. 15 (1), pp. e0238423. Date of Electronic Publication: 2023 Dec 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Society for Microbiology |
| مواضيع طبية MeSH: | Urinary Tract Infections* , Wound Infection*, Animals ; Mice ; Enterococcus faecalis/genetics ; Enterococcus faecalis/metabolism ; Carbohydrates ; Purines |
| مستخلص: | Importance: Although E. faecalis is a common wound pathogen, its pathogenic mechanisms during wound infection are unexplored. Here, combining a mouse wound infection model with in vivo transposon and RNA sequencing approaches, we identified the E. faecalis purine biosynthetic pathway and galactose/mannose MptABCD phosphotransferase system as essential for E. faecalis acute replication and persistence during wound infection, respectively. The essentiality of purine biosynthesis and the MptABCD PTS is driven by the consumption of purine metabolites by E. faecalis during acute replication and changing carbohydrate availability during the course of wound infection. Overall, our findings reveal the importance of the wound microenvironment in E. faecalis wound pathogenesis and how these metabolic pathways can be targeted to better control wound infections. Competing Interests: The authors declare no conflict of interest. |
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| معلومات مُعتمدة: | RCE-SCELSE National Research Foundation Singapore (NRF); RCE-SCELSE Ministry of Education - Singapore (MOE); MOH-OFIRG18may-005 MOH | National Medical Research Council (NMRC); 2019-T1-001-059 Ministry of Education - Singapore (MOE) |
| فهرسة مساهمة: | Keywords: Enterococcus faecalis; carbohydrates; fitness determinants; in vivo RNA sequencing; in vivo transposon sequencing; phosphotransferase system (PTS); purine biosynthesis; purines; wound infection; wound microenvironment |
| المشرفين على المادة: | 0 (Carbohydrates) 0 (Purines) |
| تواريخ الأحداث: | Date Created: 20231211 Date Completed: 20240117 Latest Revision: 20240118 |
| رمز التحديث: | 20240118 |
| مُعرف محوري في PubMed: | PMC10790769 |
| DOI: | 10.1128/mbio.02384-23 |
| PMID: | 38078746 |
| قاعدة البيانات: | MEDLINE |
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