دورية أكاديمية
Implementation of a PCR-based strategy to control an outbreak by Serratia marcescens in a Neonatal Intensive Care Unit.
| العنوان: | Implementation of a PCR-based strategy to control an outbreak by Serratia marcescens in a Neonatal Intensive Care Unit. |
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| المؤلفون: | Rodríguez-Villodres Á; Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain. anrovi1797@gmail.com.; Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, Seville, Spain. anrovi1797@gmail.com.; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC). Instituto de Salud Carlos III, Madrid, Spain. anrovi1797@gmail.com., Ortiz de la Rosa JM; Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain.; Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, Seville, Spain., Valencia-Martin R; Clinical Unit of Preventive Medicine and Public Health, University Hospital Virgen del Rocío, Seville, Spain.; Department of Preventive Medicine, University of Seville, Seville, Spain., Jiménez Parrilla F; Clinical Unit of Neonatology, University Hospital Virgen del Rocío, Seville, Spain., Martín-Gutiérrez G; Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain.; Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, Seville, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC). Instituto de Salud Carlos III, Madrid, Spain., Márquez Patiño N; Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain., Perea Cruz E; Clinical Unit of Preventive Medicine and Public Health, University Hospital Virgen del Rocío, Seville, Spain., Sánchez Jiménez MT; Clinical Unit of Neonatology, University Hospital Virgen del Rocío, Seville, Spain., Pavón Delgado A; Clinical Unit of Neonatology, University Hospital Virgen del Rocío, Seville, Spain., Cisneros JM; Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain.; Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, Seville, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC). Instituto de Salud Carlos III, Madrid, Spain.; Faculty of Medicine, University of Seville, Seville, Spain., Lepe JA; Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain.; Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, Seville, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC). Instituto de Salud Carlos III, Madrid, Spain.; Department of Microbiology, University of Seville, Seville, Spain. |
| المصدر: | Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2023 Dec 11; Vol. 22 (1), pp. 108. Date of Electronic Publication: 2023 Dec 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101152152 Publication Model: Electronic Cited Medium: Internet ISSN: 1476-0711 (Electronic) Linking ISSN: 14760711 NLM ISO Abbreviation: Ann Clin Microbiol Antimicrob Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2002]- |
| مواضيع طبية MeSH: | Cross Infection*/epidemiology , Cross Infection*/prevention & control , Cross Infection*/diagnosis , Serratia Infections*/epidemiology , Serratia Infections*/prevention & control , Serratia Infections*/diagnosis , Sepsis*/epidemiology, Infant, Newborn ; Humans ; Intensive Care Units, Neonatal ; Serratia marcescens/genetics ; Polymerase Chain Reaction ; Disease Outbreaks |
| مستخلص: | Objectives: To evaluate the clinical and epidemiological impact of a new molecular surveillance strategy based on qPCR to control an outbreak by Serratia marcescens in a Neonatal Intensive Care Unit (NICU). Methods: We design a specific qPCR for the detection of S. marcescens in rectal swabs of patients admitted to a NICU. We divided the surveillance study into two periods: (a) the pre-PCR, from the outbreak declaration to the qPCR introduction, and (b) the PCR period, from the introduction of the qPCR until the outbreak was solved. In all cases, S. marcescens isolates were recovered and their clonal relationship was analysed by PFGE. Control measures were implemented during the outbreak. Finally, the number of bloodstream infections (BSI) was investigated in order to evaluate the clinical impact of this molecular strategy. Results: Nineteen patients colonized/infected by S. marcescens were detected in the pre-PCR period (October 2020-April 2021). On the contrary, after the PCR implementation, 16 new patients were detected. The PFGE revealed 24 different pulsotypes belonging to 7 different clonal groups, that were not overlapping at the same time. Regarding the clinical impact, 18 months after the qPCR implementation, no more outbreaks by S. marcescens have been declared in the NICU of our hospital, and only 1 episode of BSI has occurred, compared with 11 BSI episodes declared previously to the outbreak control. Conclusions: The implementation of this qPCR strategy has proved to be a useful tool to control the nosocomial spread of S. marcescens in the NICU. (© 2023. The Author(s).) |
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| معلومات مُعتمدة: | JR20/00023 Instituto de Salud Carlos III; CD21/00098 Instituto de Salud Carlos III; JR19/00039 Instituto de Salud Carlos III |
| فهرسة مساهمة: | Keywords: Epidemiology; Neonatal Intensive Care Unit; Outbreak; PCR; Serratia marcescens |
| تواريخ الأحداث: | Date Created: 20231212 Date Completed: 20231216 Latest Revision: 20231216 |
| رمز التحديث: | 20231217 |
| مُعرف محوري في PubMed: | PMC10714559 |
| DOI: | 10.1186/s12941-023-00657-0 |
| PMID: | 38082303 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1476-0711 |
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| DOI: | 10.1186/s12941-023-00657-0 |