دورية أكاديمية
Ginsenoside Rd Induces Differentiation of Myeloid Leukemia Cells via Regulating ERK/GSK-3β Signaling Pathway.
| العنوان: | Ginsenoside Rd Induces Differentiation of Myeloid Leukemia Cells via Regulating ERK/GSK-3β Signaling Pathway. |
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| المؤلفون: | Jiang YX; Department of Hematology, Tongde Hospital of Zhejiang Province, Hangzhou, 310012, China., Zhao YN; Institute of Hematology Research, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China., Yu XL; Institute of Hematology Research, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China., Yin LM; Institute of Hematology Research, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China. yinlm0509@zcmu.edu.cn. |
| المصدر: | Chinese journal of integrative medicine [Chin J Integr Med] 2024 Jul; Vol. 30 (7), pp. 588-599. Date of Electronic Publication: 2023 Dec 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Chinese Journal of Integrated Traditional and Western Medicine Press Country of Publication: China NLM ID: 101181180 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1993-0402 (Electronic) Linking ISSN: 16720415 NLM ISO Abbreviation: Chin J Integr Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2007- : Berlin : Chinese Journal of Integrated Traditional and Western Medicine Press : Springer Original Publication: Beijing, China : Watertown, MA : Chinese Association of the Integration of Traditional and Western Medicine : China Academy of Traditional Chinese Medicine ; Distributed by Relaxing Natural Health, [2003]- |
| مواضيع طبية MeSH: | Ginsenosides*/pharmacology , Glycogen Synthase Kinase 3 beta*/metabolism , Cell Differentiation*/drug effects , Signal Transduction*/drug effects, Animals ; Humans ; Cell Line, Tumor ; Mice ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Cell Survival/drug effects ; Leukemia, Myeloid/pathology ; Leukemia, Myeloid/drug therapy ; Leukemia, Myeloid/metabolism ; MAP Kinase Signaling System/drug effects |
| مستخلص: | Objective: To investigate the role of ginsenoside Rd (GRd) in acute myeloid leukemia (AML) cell differentiation. Methods: AML cells were treated with GRd (25, 50, 100 and 200 µg/mL), retinoic acid (RA, 0.1g/L) and PD98059 (20 mg/mL) for 72 h, cell survival was detected by methylthiazolyldiphenyl-tetrazolium bromide and colony formation assays, and cell cycle was detected by flow cytometry. Cell morphology and differentiation were observed by Wright-Giemsa staining, peroxidase chemical staining and cellular immunochemistry assay, respectively. The protein expression levels of GATA binding protein 1 (GATA-1), purine rich Box-1 (PU.1), phosphorylated-extracellular signal-related kinase (p-ERK), ERK, phosphorylated-glycogen synthase kinase-3β (p-GSK3β), GSK3β and signal transducer and activator of transcription 1 (STAT1) were detected by Western blot. Thirty-six mice were randomly divided into 3 groups using a random number table: model control group (non-treated), GRd group [treated with 200 mg/(kg·d) GRd] and homoharringtonine (HTT) group [treated with 1 mg/(kg·d) HTT]. A tumor-bearing nude mouse model was established, and tumor weight and volume were recorded. Changes of subcutaneous tumor tissue were observed after hematoxylin and eosin staining. WT1 and GATA-1 expressions were detected by immunohistochemical staining. Results: The cell survival was inhibited by GRd in a dose-dependent manner and GRd caused G0/G1 cell arrest (p<0.05). GRd treatment induced leukemia cell differentiation, showing increased expressions of peroxidase and specific proteins concerning erythrogenic or granulocytic differentiation (p<0.05). GRd treatment elicited upregulation of p-ERK, p-GSK-3β and STAT1 expressions in cells, and reversed the effects of PD98059 on inhibiting the expressions of peroxidase, GATA-1 and PU.1 (P<0.05). After GRd treatment, tumor weight and volume of mice were decreased, and tumor cells underwent massive apoptosis and necrosis (P<0.05). WT1 level was decreased, and GATA-1 level was significantly increased in subcutaneous tumor tissues (P<0.05 or P<0.01). Conclusion: GRd might induce the differentiation of AML cells via regulating the ERK/GSK-3β signaling pathway. (© 2023. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: differentiation; extracellular signal-related kinase signaling pathway; ginsenoside Rd; myeloid leukemia; survival |
| المشرفين على المادة: | 0 (Ginsenosides) EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta) WB232T95AV (ginsenoside Rd) EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) |
| تواريخ الأحداث: | Date Created: 20231212 Date Completed: 20240627 Latest Revision: 20240719 |
| رمز التحديث: | 20240719 |
| DOI: | 10.1007/s11655-023-3561-z |
| PMID: | 38085388 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1993-0402 |
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| DOI: | 10.1007/s11655-023-3561-z |