دورية أكاديمية
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Testing a Method for Evaluation of the Viability of Biofilm-Forming Bacteria after Exposure to Disinfectants.

التفاصيل البيبلوغرافية
العنوان: Testing a Method for Evaluation of the Viability of Biofilm-Forming Bacteria after Exposure to Disinfectants.
المؤلفون: Nemchenko UM; Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia. umnemch@mail.ru., Voropaeva NM; Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia., Sitnikova KO; Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia., Belkova NL; Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia., Savilov ED; Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.; Irkutsk State Medical Academy of Postgraduate Education, Branch of Russian Medical Academy of Continuous Professional Education, Ministry of Health of the Russian Federation, Moscow, Russia.
المصدر: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Nov; Vol. 176 (1), pp. 60-63. Date of Electronic Publication: 2023 Dec 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 0372557 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-8221 (Electronic) Linking ISSN: 00074888 NLM ISO Abbreviation: Bull Exp Biol Med Subsets: MEDLINE
أسماء مطبوعة: Publication: New York : Springer
Original Publication: New York, Consultants Bureau.
مواضيع طبية MeSH: Disinfectants*/pharmacology, Quaternary Ammonium Compounds/pharmacology ; Formazans ; Bacteria ; Biofilms
مستخلص: A method for determining the viability of opportunistic pathogenic bacteria at the stage of biofilm formation after exposure to disinfectants with different active components was tested. The method is based on oxidation of tetrazolium salts by metabolically active cells with the formation of colored formazan derivatives and their quantitative spectrophotometry. The cell viability in the biofilm decreased after exposure to quaternary ammonium compounds and chlorine-containing disinfectants, but their effect was reversible. Dissemination of cells that had retained viability from the biofilm occurred after 24 h. The algorithm of testing, necessary controls, counting, and data interpretation are specified. The method can be recommended for use in laboratory diagnostics and clinical practice.
(© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)
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فهرسة مساهمة: Keywords: XTT reagent; biofilm; cell viability; disinfectants
المشرفين على المادة: 0 (Disinfectants)
0 (Quaternary Ammonium Compounds)
0 (Formazans)
تواريخ الأحداث: Date Created: 20231213 Date Completed: 20231219 Latest Revision: 20231219
رمز التحديث: 20231219
DOI: 10.1007/s10517-023-05967-z
PMID: 38091139
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-8221
DOI:10.1007/s10517-023-05967-z