دورية أكاديمية
Spliceosome component PHD finger 5A is essential for early B lymphopoiesis.
| العنوان: | Spliceosome component PHD finger 5A is essential for early B lymphopoiesis. |
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| المؤلفون: | Zhang R; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Wang D; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Ruan GX; Medical School, Taizhou University, Taizhou 318000, China., Wang R; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Li Y; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Chen W; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Huang H; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Wang J; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Meng L; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Zhu Z; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Lei D; Department of Ophthalmology, Southern University of Science and Technology Hospital, Shenzhen 518055, China., Xu S; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, Immunos, Singapore 138648, Republic of Singapore.; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 2 Medical Drive MD9, Singapore 117593, Republic of Singapore., Ou X; Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China. |
| المصدر: | Development (Cambridge, England) [Development] 2024 Jan 15; Vol. 151 (2). Date of Electronic Publication: 2024 Jan 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 8701744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9129 (Electronic) Linking ISSN: 09501991 NLM ISO Abbreviation: Development Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Eng : Company Of Biologists Limited Original Publication: [Cambridge] : Company of Biologists, [c1987- |
| مواضيع طبية MeSH: | Spliceosomes*/metabolism , Trans-Activators*/genetics, Animals ; Mice ; RNA-Binding Proteins/metabolism ; PHD Zinc Fingers ; Lymphopoiesis/genetics |
| مستخلص: | The spliceosome, a multi-megadalton ribonucleoprotein complex, is essential for pre-mRNA splicing in the nucleus and ensuring genomic stability. Its precise and dynamic assembly is pivotal for its function. Spliceosome malfunctions can lead to developmental abnormalities and potentially contribute to tumorigenesis. The specific role of the spliceosome in B cell development is poorly understood. Here, we reveal that the spliceosomal U2 snRNP component PHD finger protein 5A (Phf5a) is vital for early B cell development. Loss of Phf5a results in pronounced defects in B cell development, causing an arrest at the transition from pre-pro-B to early pro-B cell stage in the bone marrow of mutant mice. Phf5a-deficient B cells exhibit impaired immunoglobulin heavy (IgH) chain expression due to defective V-to-DJ gene rearrangement. Mechanistically, our findings suggest that Phf5a facilitates IgH gene rearrangement by regulating the activity of recombination-activating gene endonuclease and influencing chromatin interactions at the Igh locus. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
| معلومات مُعتمدة: | 32170882 National Natural Science Foundation of China; JCYJ20220530115212028 Shenzhen Fundamental Research Program; Singapore Immunology Network; Agency for Science, Technology and Research |
| فهرسة مساهمة: | Keywords: VDJ gene recombination; B cell development; Chromatin looping; Phf5a; RNA splicing |
| المشرفين على المادة: | 0 (Trans-Activators) 0 (RNA-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20231214 Date Completed: 20240112 Latest Revision: 20240112 |
| رمز التحديث: | 20240112 |
| DOI: | 10.1242/dev.202247 |
| PMID: | 38095286 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9129 |
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| DOI: | 10.1242/dev.202247 |