دورية أكاديمية
أعرض في EDS

ACE2 activation alleviates sepsis-induced cardiomyopathy by promoting MasR-Sirt1-mediated mitochondrial biogenesis.

التفاصيل البيبلوغرافية
العنوان: ACE2 activation alleviates sepsis-induced cardiomyopathy by promoting MasR-Sirt1-mediated mitochondrial biogenesis.
المؤلفون: Wan TT; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China., Li Y; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China., Li JX; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China., Xiao X; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China., Liu L; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China., Li HH; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China. Electronic address: lihuihua@bjcyh.com., Guo SB; Department of Emergency Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, China. Electronic address: guoshubin@bjcyh.com.
المصدر: Archives of biochemistry and biophysics [Arch Biochem Biophys] 2024 Feb; Vol. 752, pp. 109855. Date of Electronic Publication: 2023 Dec 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372430 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0384 (Electronic) Linking ISSN: 00039861 NLM ISO Abbreviation: Arch Biochem Biophys Subsets: MEDLINE
أسماء مطبوعة: Publication: <2000- > : San Diego, CA : Elsevier
Original Publication: New York, NY : Academic Press
مواضيع طبية MeSH: Cardiomyopathies*/drug therapy , Cardiomyopathies*/etiology , Sepsis*/complications , Sepsis*/drug therapy, Animals ; Mice ; Angiotensin-Converting Enzyme 2 ; Mice, Inbred C57BL ; Organelle Biogenesis ; Peptidyl-Dipeptidase A ; Sirtuin 1
مستخلص: Sepsis-induced cardiomyopathy (SIC), caused by a dysregulated host response to infection, is a major contributor to high mortality. Angiotensin-converting enzyme 2 (ACE2), a crucial component of the renin-angiotensin system (RAS), has protective effects against several cardiovascular diseases, such as myocardial infarction and heart failure. However, the role of ACE2 in the pathogenesis of SIC and underlying mechanisms remain unknown. The present study was designed to examine the effects of ACE2 activation or inhibition on SIC in C57BL/6 mice. The ACE2 activator diminazene aceturate (DIZE) and ACE2 inhibitor MLN-4760 were applied for treatment. Myocardial function, inflammatory response, oxidative stress, apoptosis and mitochondrial biogenesis were investigated. Major assays were echocardiography, H&E staining, immunofluorescence staining, DHE staining, TUNEL staining, Western blot, qPCR analysis, ELISA and corresponding kits. We confirmed that ACE2 was markedly downregulated in septic heart tissues. Pharmacological activation of ACE2 by DIZE ameliorated cecal ligation puncture (CLP)-induced mortality, cardiac dysfunction, inflammatory response, oxidative stress and the cardiomyocyte apoptosis by promoting MasR-Sirt1-mediated mitochondrial biogenesis. In contrast, SIC was aggravated via inhibiting MasR-Sirt1-mediated mitochondrial biogenesis by the use of ACE2 inhibitor MLN-4760. Consequently, activation of ACE2 may protect against SIC by promoting MasR-Sirt1-mediated mitochondrial biogenesis.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: ACE2; Cecal ligation puncture; MasR-Sirt1; Mitochondrial biogenesis; Sepsis-induced cardiomyopathy
المشرفين على المادة: EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
EC 3.4.15.1 (Peptidyl-Dipeptidase A)
EC 3.5.1.- (Sirt1 protein, mouse)
EC 3.5.1.- (Sirtuin 1)
0 (Mas1 protein, mouse)
EC 3.4.17.23 (Ace2 protein, mouse)
تواريخ الأحداث: Date Created: 20231214 Date Completed: 20240201 Latest Revision: 20240223
رمز التحديث: 20240223
DOI: 10.1016/j.abb.2023.109855
PMID: 38097099
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-0384
DOI:10.1016/j.abb.2023.109855