دورية أكاديمية
The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2.
العنوان: | The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2. |
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المؤلفون: | Douglas M; The University of Manchester, UK., O'Loughlin C; The University of Manchester, UK., Lynch AT; The University of Manchester, UK., Prialgauskaite R; The University of Manchester, UK., Adamson AD; The University of Manchester, UK., Dibb KM; The University of Manchester, UK., Kimber SJ; The University of Manchester, UK., Birket MJ; The University of Manchester, UK. Electronic address: matthew.birket@manchester.ac.uk. |
المصدر: | Stem cell research [Stem Cell Res] 2024 Feb; Vol. 74, pp. 103262. Date of Electronic Publication: 2023 Dec 03. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101316957 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7753 (Electronic) Linking ISSN: 18735061 NLM ISO Abbreviation: Stem Cell Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Kidlington, Oxford : Elsevier |
مواضيع طبية MeSH: | Human Embryonic Stem Cells*/metabolism, Humans ; Cell Differentiation ; Embryonic Stem Cells/metabolism ; Myocytes, Cardiac/metabolism ; Cell Line ; Homeobox Protein Nkx-2.5/genetics ; Homeobox Protein Nkx-2.5/metabolism |
مستخلص: | The transcription factor NKX2-5 is a highly conserved master regulator of heart development which is widely expressed in cardiac progenitors and cardiomyocytes. Fluorescent reporters of NKX2-5 that minimally perturb normal protein expression can enable the identification, quantification and isolation of NKX2-5-expressing cells in a normal physiological state. Here we report the generation of two new hESC lines with eGFP inserted upstream (5') or downstream (3') of NKX2-5, linked by a cleavable T2A peptide. These complementary reporters produce a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Matthew Birket reports financial support was provided by UK Research and Innovation. Katharine Dibb reports financial support was provided by British Heart Foundation. (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.) |
معلومات مُعتمدة: | MR/K026666/1 United Kingdom MRC_ Medical Research Council; MR/T041668/1 United Kingdom MRC_ Medical Research Council |
فهرسة مساهمة: | Keywords: NKX2-5; cardiac; cardiomyocyte; pluripotent stem cell; progenitor; reporter |
المشرفين على المادة: | 0 (NKX2-5 protein, human) 0 (Homeobox Protein Nkx-2.5) |
تواريخ الأحداث: | Date Created: 20231215 Date Completed: 20240115 Latest Revision: 20240320 |
رمز التحديث: | 20240320 |
DOI: | 10.1016/j.scr.2023.103262 |
PMID: | 38100908 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1876-7753 |
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DOI: | 10.1016/j.scr.2023.103262 |