دورية أكاديمية
أعرض في EDS

Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics.

التفاصيل البيبلوغرافية
العنوان: Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics.
المؤلفون: Gaspari M; Soil and Water Resources Institute, Hellenic Agricultural Organisation Dimitra, Thermi, Thessaloniki 57001, Greece.; Department of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece., Ghiotto G; Department of Biology, University of Padova, Padova 35121, Italy., Centurion VB; Department of Biology, University of Padova, Padova 35121, Italy., Kotsopoulos T; Department of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece., Santinello D; Department of Biology, University of Padova, Padova 35121, Italy., Campanaro S; Department of Biology, University of Padova, Padova 35121, Italy., Treu L; Department of Biology, University of Padova, Padova 35121, Italy., Kougias PG; Soil and Water Resources Institute, Hellenic Agricultural Organisation Dimitra, Thermi, Thessaloniki 57001, Greece.
المصدر: Environmental science & technology [Environ Sci Technol] 2024 Jan 09; Vol. 58 (1), pp. 591-602. Date of Electronic Publication: 2023 Dec 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0213155 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5851 (Electronic) Linking ISSN: 0013936X NLM ISO Abbreviation: Environ Sci Technol Subsets: MEDLINE
أسماء مطبوعة: Publication: Washington DC : American Chemical Society
Original Publication: Easton, Pa. : American Chemical Society, c1967-
مواضيع طبية MeSH: Ammonia*/metabolism , Ammonia*/pharmacology , Microbiota*, Biofuels ; Bioreactors ; Metagenome ; Anaerobiosis ; Methane ; Metagenomics
مستخلص: The presence of elevated ammonia levels is widely recognized as a significant contributor to process inhibition in biogas production, posing a common challenge for biogas plant operators. The present study employed a combination of biochemical, genome-centric metagenomic and metatranscriptomic data to investigate the response of the biogas microbiome to two shock loads induced by single pulses of elevated ammonia concentrations (i.e., 1.5 g NH 4 + /L R and 5 g NH 4 + /L R ). The analysis revealed a microbial community of high complexity consisting of 364 Metagenome Assembled Genomes (MAGs). The hydrogenotrophic pathway was the primary route for methane production during the entire experiment, confirming its efficiency even at high ammonia concentrations. Additionally, metatranscriptomic analysis uncovered a metabolic shift in the methanogens Methanothrix sp. MA6 and Methanosarcina flavescens MX5, which switched their metabolism from the acetoclastic to the CO 2 reduction route during the second shock. Furthermore, multiple genes associated with mechanisms for maintaining osmotic balance in the cell were upregulated, emphasizing the critical role of osmoprotection in the rapid response to the presence of ammonia. Finally, this study offers insights into the transcriptional response of an anaerobic digestion community, specifically focusing on the mechanisms involved in recovering from ammonia-induced stress.
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فهرسة مساهمة: Keywords: ammonia shock; anaerobic digestion; metagenomics; metatranscriptomics; methanogenesis; microbiome; osmotic balance
المشرفين على المادة: 7664-41-7 (Ammonia)
0 (Biofuels)
OP0UW79H66 (Methane)
تواريخ الأحداث: Date Created: 20231219 Date Completed: 20240110 Latest Revision: 20240114
رمز التحديث: 20240114
مُعرف محوري في PubMed: PMC10785759
DOI: 10.1021/acs.est.3c07840
PMID: 38112274
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5851
DOI:10.1021/acs.est.3c07840