دورية أكاديمية
[Inhibition of GMA-induced ubiquitination process in 16HBE malignantly transformed cells on protein CD44 and MMP14].
العنوان: | [Inhibition of GMA-induced ubiquitination process in 16HBE malignantly transformed cells on protein CD44 and MMP14]. |
---|---|
المؤلفون: | Jin H; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Wang Q; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China State Key Laboratory of Trauma and Chemical Poisoning, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Gu Y; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Wuhan B; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Li X; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China State Key Laboratory of Trauma and Chemical Poisoning, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Cui X; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Zhang L; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China State Key Laboratory of Trauma and Chemical Poisoning, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Xu J; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China State Key Laboratory of Trauma and Chemical Poisoning, Chinese Center for Disease Control and Prevention, Beijing 100050, China. |
المصدر: | Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2023 Nov; Vol. 52 (6), pp. 871-876. |
نوع المنشور: | English Abstract; Journal Article |
اللغة: | Chinese |
بيانات الدورية: | Publisher: |
أسماء مطبوعة: | Publication: Beijing Shi : "Wei sheng yan jiu" bian ji bu Original Publication: Beijing Shi : "Wei sheng yan jiu" bian ji bu, |
مواضيع طبية MeSH: | Epithelial Cells* , Matrix Metalloproteinase 14*/metabolism , Matrix Metalloproteinase 14*/pharmacology, Humans ; Agar/adverse effects ; Agar/metabolism ; Hyaluronan Receptors/metabolism ; Ubiquitination ; Solvents/adverse effects ; Solvents/metabolism ; Cell Transformation, Neoplastic/chemically induced |
مستخلص: | Objective: To observe the effect of the ubiquitination process on the expression of CD44 antigen(CD44) and matrix metalloproteinase-14(MMP14) in human bronchial epithelial(16HBE) malignantly transformed cells induced by glycidyl methacrylate(GMA). Methods: Successfully resuscitated 16HBE cells were cultured using a final concentration of 8 μg/mL GMA as the treatment group and 1 μg/mL dimethyl sulfoxide as the solvent control group, each time stained for 72 h, and then stained again after an interval of 24 h. After repeating the staining three times, the cells were cultured in passages respectively. The 40th generation(P40) GMA-treated group and the same-generation solvent control group were subjected to soft agar colony formation assay and concanavalin A(ConA) agglutination test to confirm that the 40th generation of GMA-induced malignant transformed 16HBE cells possessed malignant transformed cell characteristics.5, 10, 20, 40, 60 μmol/L anacardic acid were used to inhibit the ubiquitination process of GMA-induced malignant transformed 16HBE cells. The protein expression of CD44 and MMP14 were detected by western blotting, while the transcript levels of CD44, MMP14, and TFAP2A were assessed by real-time fluorescence quantitative PCR(qPCR). Results: (1) In the soft agar colony formation assay, the number of clones formed by the cells in the solvent control group was 22, and the number of clones created by the malignantly transformed cells in the GMA-treated group was 208. In the ConA agglutination test, the cells in the solvent control group were uniformly dispersed in ConA solution, and no obvious agglutination occurred for 30 min, whereas the cells in the GMA-treated group were agglutinated in the 5th min, and the agglutinated cells were larger and more rapidly agglutinated. The agglomerates were more significant and faster, and the sensitivity of agglutination was increased. (2) After differential inhibition of GMA-induced ubiquitination in malignantly transformed 16HBE cells, the expression levels of CD44 and MMP14 were reduced in GMA-induced malignantly transformed 16HBE cells compared with the control group(P<0.05). The transcript levels of MMP14 and CD44 decreased with increasing inhibitor concentration(P<0.05), and the transcript levels of the upstream transcription factor TFAP2A were also simultaneously reduced(P<0.05). Conclusion: Inhibition of the cellular ubiquitination process mediates the down-regulation of protein expression and transcriptional expression of CD44 and MMP14 in GMA-induced malignantly transformed 16HBE cells. |
فهرسة مساهمة: | Keywords: 16HBE cells; glycidyl methacrylate; malignant transformation; ubiquitination process |
المشرفين على المادة: | EC 3.4.24.80 (Matrix Metalloproteinase 14) 9002-18-0 (Agar) 0 (Hyaluronan Receptors) 0 (Solvents) EC 3.4.24.80 (MMP14 protein, human) 0 (CD44 protein, human) |
تواريخ الأحداث: | Date Created: 20231220 Date Completed: 20231221 Latest Revision: 20231221 |
رمز التحديث: | 20231221 |
DOI: | 10.19813/j.cnki.weishengyanjiu.2023.06.002 |
PMID: | 38115649 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1000-8020 |
---|---|
DOI: | 10.19813/j.cnki.weishengyanjiu.2023.06.002 |