دورية أكاديمية
High-Throughput 3D Imaging Flow Cytometry of Suspended Adherent 3D Cell Cultures.
| العنوان: | High-Throughput 3D Imaging Flow Cytometry of Suspended Adherent 3D Cell Cultures. |
|---|---|
| المؤلفون: | Yamashita M; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan., Tamamitsu M; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan., Kirisako H; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan., Goda Y; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan., Chen X; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan., Hattori K; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan., Ota S; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. |
| المصدر: | Small methods [Small Methods] 2024 Aug; Vol. 8 (8), pp. e2301318. Date of Electronic Publication: 2023 Dec 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: WILEY-VCH Verlag GmbH & Co. KGaA Country of Publication: Germany NLM ID: 101724536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2366-9608 (Electronic) Linking ISSN: 23669608 NLM ISO Abbreviation: Small Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : WILEY-VCH Verlag GmbH & Co. KGaA, [2017]- |
| مواضيع طبية MeSH: | Spheroids, Cellular*/cytology , Flow Cytometry*/methods , Cell Culture Techniques, Three Dimensional*/methods , Imaging, Three-Dimensional*/methods, Humans ; Hydrogels/chemistry ; High-Throughput Screening Assays/methods ; Cell Adhesion ; Lab-On-A-Chip Devices ; Cell Culture Techniques/methods ; Cell Culture Techniques/instrumentation ; Cell Survival |
| مستخلص: | 3D cell cultures are indispensable in recapitulating in vivo environments. Among the many 3D culture methods, culturing adherent cells on hydrogel beads to form spheroid-like structures is a powerful strategy for maintaining high cell viability and functions in the adherent states. However, high-throughput, scalable technologies for 3D imaging of individual cells cultured on the hydrogel scaffolds are lacking. This study reports the development of a high throughput, scalable 3D imaging flow cytometry platform for analyzing spheroid models. This platform is realized by integrating a single objective fluorescence light-sheet microscopy with a microfluidic device that combines hydrodynamic and acoustofluidic focusing techniques. This integration enabled unprecedentedly high-throughput and scalable optofluidic 3D imaging, processing 1310 spheroids consisting of 28 117 cells min -1 . The large dataset obtained enables precise quantification and comparison of the nuclear morphology of adhering and suspended cells, revealing that the adhering cells have smaller nuclei with less rounded surfaces. This platform's high throughput, robustness, and precision for analyzing the morphology of subcellular structures in 3D culture models hold promising potential for various biomedical analyses, including image-based phenotypic screening of drugs with spheroids or organoids. (© 2023 The Authors. Small Methods published by Wiley‐VCH GmbH.) |
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| معلومات مُعتمدة: | Tateisi Science and Technology Foundation; JP21H04636 Japan Society for the Promotion of Science; JP21H00416 Japan Society for the Promotion of Science; JP22K12797 Japan Society for the Promotion of Science; JPMJCR19H1 Japan Science and Technology Agency; Japan Society for the Study of Obesity; Cell Science Research Foundation; Takeda Science Foundation; UTEC-UTokyo FSI Foundation; Secom Science and Technology Foundation; The Canon Foundation; White Rock Foundation; Uehara Memorial Foundation; JP22gm6710008 Japan Agency for Medical Research and Development, AMED; Inamori Foundation |
| فهرسة مساهمة: | Keywords: 3D cell culture; 3D imaging flow cytometry; acoustofluidics; adherent cell; hydrogel bead; light‐sheet microscopy |
| المشرفين على المادة: | 0 (Hydrogels) |
| تواريخ الأحداث: | Date Created: 20231222 Date Completed: 20240817 Latest Revision: 20240817 |
| رمز التحديث: | 20240818 |
| DOI: | 10.1002/smtd.202301318 |
| PMID: | 38133483 |
| قاعدة البيانات: | MEDLINE |
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