دورية أكاديمية
Red bioactive pigment from Himalayan Janthinobacterium sp. ERMR3:09: optimization, characterization, and potential applications.
| العنوان: | Red bioactive pigment from Himalayan Janthinobacterium sp. ERMR3:09: optimization, characterization, and potential applications. |
|---|---|
| المؤلفون: | Mukhia S; High Altitude Microbiology Laboratory (HAM-LAB), Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur, Himachal Pradesh, 176061, India.; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India., Kumar A; High Altitude Microbiology Laboratory (HAM-LAB), Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur, Himachal Pradesh, 176061, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India., Kumar R; High Altitude Microbiology Laboratory (HAM-LAB), Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur, Himachal Pradesh, 176061, India. rakshak@ihbt.res.in.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. rakshak@ihbt.res.in. |
| المصدر: | Archives of microbiology [Arch Microbiol] 2023 Dec 27; Vol. 206 (1), pp. 44. Date of Electronic Publication: 2023 Dec 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Prodigiosin* , Anti-Infective Agents*/pharmacology , Anti-Infective Agents*/metabolism, Humans ; HeLa Cells ; Serratia marcescens ; Temperature |
| مستخلص: | Prodigiosin is a red pigment commonly produced as a secondary metabolite by Serratia marcescens. It exhibits inherent bioactivities, including antimicrobial and anticancer, with low to no toxic effects on normal cells. The present study investigates a bioactive prodigiosin production from an atypical, red-pigmented, potentially novel Janthinobacterium sp. ERMR3:09 isolated from a glacial moraine. Statistically optimized culture parameters, i.e., w/v 1.0% glucose and 0.08% peptone as carbon and nitrogen sources, temperature 20 °C, and media pH 7, resulted in a four-fold increase in the pigment yield. The upscaled production in an 8 L volume resulted in higher pigment production within a shorter period of 48 h. The ultra-performance liquid chromatography (UPLC) analysis validated the identity of the purified pigment as prodigiosin that showed thermostability at 75 °C for 3 h. Evaluation of antimicrobial activity showed potent inhibitory effects (> 50%) against the opportunistic pathogenic fungal and Gram-positive bacterial strains. The pigment showed significant cytotoxicity (p < 0.05) towards A549 and HeLa cell lines with IC (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| References: | Afra S, Makhdoumi A, Matin MM, Feizy J (2017) A novel red pigment from marine Arthrobacter sp. G20 with specific anticancer activity. J Appl Microbiol 123(5):1228–1236. (PMID: 2886278310.1111/jam.13576) Anwar MM, Albanese C, Hamdy NM, Sultan AS (2022) Rise of the natural red pigment ‘prodigiosin’ as an immunomodulator in cancer. Cancer Cell Int 22(1):419. (PMID: 36577970979866110.1186/s12935-022-02815-4) Balasubramaniam B, Alexpandi R, Darjily DR (2019) Exploration of the optimized parameters for bioactive prodigiosin mass production and its biomedical applications in vitro as well as in silico. Biocatal Agric Biotechnol 22:101385. (PMID: 10.1016/j.bcab.2019.101385) Bhagwat A, Padalia U (2020) Optimization of prodigiosin biosynthesis by Serratia marcescens using unconventional bioresources. J Genet Eng Biotechnol 18(1):1–9. (PMID: 10.1186/s43141-020-00045-7) Bisht G, Srivastava S, Kulshreshtha R, Sourirajan A, Baumler DJ, Dev K (2020) Applications of red pigments from psychrophilic Rhodonellum psychrophilum GL8 in health, food and antimicrobial finishes on textiles. Process Biochem 94:15–29. (PMID: 10.1016/j.procbio.2020.03.021) Brehl C, Brass HUC, Lüchtrath C, Böckmann L, Ihling N, Classen T, Büchs J et al (2022) Optimized prodigiosin production with Pseudomonas putida KT2440 using parallelized noninvasive online monitoring. Biotechnol Prog 38:e3245. (PMID: 3517026010.1002/btpr.3245) Elkenawy NM, Yassin AS, Elhifnawy HN, Amin MA (2017) Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation. Biotechnol Rep 14:47–53. (PMID: 10.1016/j.btre.2017.04.001) Giri AV, Anandkumar N, Muthukumaran G, Pennathur G (2004) A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol 4(1):1–10. (PMID: 10.1186/1471-2180-4-11) Gohil N, Bhattacharjee G, Singh V (2020) Synergistic bactericidal profiling of prodigiosin extracted from Serratia marcescens in combination with antibiotics against pathogenic bacteria. Microb Pathog 149:104508. (PMID: 32956792749909210.1016/j.micpath.2020.104508) Gondil VS, Asif M, Bhalla TC (2017) Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity. 3 Biotech 7(5):1–8. (PMID: 10.1007/s13205-017-0979-z) Han R, Xiang R, Li J, Wang F, Wang C (2021) High-level production of microbial prodigiosin: a review. J Basic Microbiol 61(6):506–523. (PMID: 3395503410.1002/jobm.202100101) Hazarika DJ, Kakoti M, Kalita R, Gautom T, Goswami G, Barooah M, Boro RC (2021) Prodigiosin from an endofungal bacterium Serratia marcescens D1 inhibits biofilm formation in gram-positive bacteria. Microbiology 90(6):829–838. (PMID: 10.1134/S0026261722010052) Ibrahim D, Nazari TF, Kassim J, Lim S-H (2014) Prodigiosin-an antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria. J Appl Pharm Sci 4(10):1–6. (PMID: 10.7324/JAPS.2014.401001) Islan GA, Rodenak-Kladniew B, Noacco N, Duran N, Castro GR (2022) Prodigiosin: a promising biomolecule with many potential biomedical applications. Bioengineered 13(6):14227–14258. (PMID: 35734783934224410.1080/21655979.2022.2084498) Jardak M, Atoissi A, Msalbi D, Atoui D, Bouizgarne B, Rigane G, Mnif S et al (2022) Antibacterial, antibiofilm and cytotoxic properties of prodigiosin produced by a newly isolated Serratia sp. C6LB from a milk collection center. Microbial Pathog 164:105449. (PMID: 10.1016/j.micpath.2022.105449) Jeong Y, Kim HJ, Kim S, Park S-Y, Kim H, Jeong S, Lee SJ (2021) Enhanced large-scale production of Hahella chejuensis-derived prodigiosin and evaluation of its bioactivity. J Microbiol Biotechnol 31(12):1624. (PMID: 34675142970590810.4014/jmb.2109.09039) Kinghorn AD, Chin Y-W, Swanson SM (2009) Discovery of natural product anticancer agents from biodiverse organisms. Curr Opin Drug Discov Dev 12(2):189. Koyun MT, Sirin S, Aslim B, Taner G, Dolanbay SN (2022) Characterization of prodigiosin pigment by Serratia marcescens and the evaluation of its bioactivities. Toxicol in Vitro 82:105368. (PMID: 10.1016/j.tiv.2022.105368) Lapenda JC, Silva PA, Vicalvi MC, Sena K, Nascimento SC (2015) Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398. World J Microbiol Biotechnol 31:399–406. (PMID: 2554990610.1007/s11274-014-1793-y) Lapenda JCL, Alves VP, Adam ML, Rodrigues MD, Nascimento SC (2020) Cytotoxic effect of prodigiosin, natural red pigment, isolated from Serratia marcescens UFPEDA 398. Indian J Microbiol 60:182–195. (PMID: 32255851710554510.1007/s12088-020-00859-6) Lazic J, Skaro Bogojevic S, Vojnovic S, Aleksic I, Milivojevic D, Kretzschmar M, Nikodinovic-Runic J et al (2022) Synthesis, anticancer potential and comprehensive toxicity studies of novel brominated derivatives of bacterial biopigment prodigiosin from Serratia marcescens ATCC 27117. Molecules 27(12):3729. (PMID: 35744855922701310.3390/molecules27123729) Lin C, Jia X, Fang Y, Chen L, Zhang H, Lin R, Chen J (2019) Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets. Electron J Biotechnol 40:58–64. (PMID: 10.1016/j.ejbt.2019.04.007) Lin SR, Chen YH, Tseng FJ, Weng CF (2020) The production and bioactivity of prodigiosin: quo vadis? Drug Discov Today 25(5):828–836. (PMID: 3225177610.1016/j.drudis.2020.03.017) Liu W, Yang J, Tian Y, Zhou X, Wang S, Zhu J, Liu C et al (2021) An in situ extractive fermentation strategy for enhancing prodigiosin production from Serratia marcescens BWL1001 and its application to inhibiting the growth of Microcystis aeruginosa. Biochem Eng J 166:107836. (PMID: 10.1016/j.bej.2020.107836) Mukhia S, Kumar A, Kumari P, Kumar R (2022) Psychrotrophic plant beneficial bacteria from the glacial ecosystem of Sikkim Himalaya: genomic evidence for the cold adaptation and plant growth promotion. Microbiol Res 260:127049. (PMID: 3550423610.1016/j.micres.2022.127049) Mukhia S, Kumar A, Kumar R (2023) Antioxidant prodigiosin producing cold-adapted Janthinobacterium sp. ERMR3: 09 from a glacier moraine: genomic elucidation of cold adaptation and pigment biosynthesis. Gene 857:147178. (PMID: 3662709210.1016/j.gene.2023.147178) Nguyen VB, Nguyen DN, Nguyen AD, Ngo VA, Ton TQ, Doan CT, Wang SL et al (2020) Utilization of crab waste for cost-effective bioproduction of prodigiosin. Mar Drugs 18(11):523. (PMID: 33105706769039710.3390/md18110523) Nguyen TH, Wang SL, Nguyen DN, Nguyen AD, Nguyen TH, Doan MD, Nguyen VB et al (2021) Bioprocessing of marine chitinous wastes for the production of bioactive prodigiosin. Molecules 26(11):3138. (PMID: 34073944819734010.3390/molecules26113138) Nguyen SLT, Nguyen TC, Do TT, Vu TL, Nguyen TT, Do TT, Nguyen TAT (2022) Study on the anticancer activity of prodigiosin from variants of Serratia marcescens QBN VTCC 910026. BioMed Res Int 2022. O’Brien K, Perron GG, Jude BA (2018) Draft genome sequence of a red-pigmented Janthinobacterium sp. native to the Hudson Valley watershed. Genome Announc 6(1):e01429-e1517. (PMID: 29301893575450210.1128/genomeA.01429-17) Olukanni OD, Abiola T, Dada JB, Dare PA, Ayoade F, Olukanni AT (2023) Resourcefulness of propylprodigiosin isolated from Brevundimonas olei strain RUN-D1. AMB Express 13(1):71. (PMID: 374228471032997010.1186/s13568-023-01579-y) Paul T, Bandyopadhyay TK, Mondal A, Tiwari ON, Muthuraj M, Bhunia B (2020) A comprehensive review on recent trends in production, purification, and applications of prodigiosin. Biomass Convers Biorefin 1–23. Paul T, Mondal A, Bandyopadhyay TK, Bhunia B (2022) Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis. Biomass Convers Biorefin 1–20. Rakh RR, Dalvi SM, Musle BB, Raut LS (2017) Production, extraction and characterization of red pigment produced by Serratia rubidaea JCM 1240T isolated from soil. Int J Curr Microbiol Appl Sci 6(1):143–154. (PMID: 10.20546/ijcmas.2017.601.018) Romanowski EG, Lehner KM, Martin NC, Patel KR, Callaghan JD, Stella NA, Shanks RMQ (2019) Thermoregulation of prodigiosin biosynthesis by is controlled at the transcriptional level and requires HexS. Pol J Microbiol 68(1):43–50. (PMID: 31050252694398410.21307/pjm-2019-005) Sajjad W, Ahmad S, Aziz I, Azam SS, Hasan F, Shah AA (2018) Antiproliferative, antioxidant and binding mechanism analysis of prodigiosin from newly isolated radio-resistant Streptomyces sp. strain WMA-LM31. Mol Biol Rep 45(6):1787–1798. (PMID: 3030650810.1007/s11033-018-4324-3) Sajjad W, Din G, Rafiq M, Iqbal A, Khan S, Zada S, Kang S et al (2020) Pigment production by cold-adapted bacteria and fungi: colorful tale of cryosphere with wide range applications. Extremophiles 24(4):447–473. (PMID: 32488508726612410.1007/s00792-020-01180-2) Schloss PD, Allen HK, Klimowicz AK, Mlot C, Gross JA, Savengsuksa S, Handelsman J et al (2010) Psychrotrophic strain of Janthinobacterium lividum from a cold Alaskan soil produces prodigiosin. DNA Cell Biol 29(9):533–541. (PMID: 20626288646894210.1089/dna.2010.1020) Sessler JL, Eller LR, Cho W, Nicolaou S, Aguilar A, Lee JT, Magda DJ et al (2005) Synthesis, anion-binding properties, and in vitro anticancer activity of prodigiosin analogues. Angew Chem 117(37):6143–6146. (PMID: 10.1002/ange.200501740) Su WT, Tsou TY, Liu HL (2011) Response surface optimization of microbial prodigiosin production from Serratia marcescens. J Taiwan Inst Chem Eng 42(2):217–222. (PMID: 10.1016/j.jtice.2010.05.009) Sudhakar C, Shobana C, Selvankumar T, Selvam K (2022) Prodigiosin production from Serratia marcescens strain CSK and their antioxidant, antibacterial, cytotoxic effect and in silico study of caspase-3 apoptotic protein. Biotechnol Appl Biochem 69(5):1984–1997. (PMID: 3458258810.1002/bab.2261) Venil CK, Zakaria ZA, Usha R, Ahmad WA (2014) Isolation and characterization of flexirubin type pigment from Chryseobacterium sp. UTM-3T. Biocatal Agric Biotechnol 3(4):103–107. (PMID: 10.1016/j.bcab.2014.02.006) Wei L, Ran J, Li Z, Zhang Q, Guo K, Mu S, Xiao Y et al (2023) Chemical composition, antibacterial activity and mechanism of action of fermentation products from Aspergillus niger xj. Appl Biochem Biotechnol 1–18. Yip CH, Yarkoni O, Ajioka J, Wan KL, Nathan S (2019) Recent advancements in high-level synthesis of the promising clinical drug, prodigiosin. Appl Microbiol Biotechnol 103(4):1667–1680. (PMID: 3063749510.1007/s00253-018-09611-z) Yip C-H, Mahalingam S, Wan K-L, Nathan S (2021) Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition. PLoS ONE 16(6):e0253445. (PMID: 34161391822149510.1371/journal.pone.0253445) |
| فهرسة مساهمة: | Keywords: Anticancer; Antimicrobial; East Rathong Glacier; Janthinobacterium; Prodigiosin; Statistical optimization |
| المشرفين على المادة: | OL369FU7CJ (Prodigiosin) 0 (Anti-Infective Agents) |
| تواريخ الأحداث: | Date Created: 20231227 Date Completed: 20231229 Latest Revision: 20240122 |
| رمز التحديث: | 20240123 |
| DOI: | 10.1007/s00203-023-03779-3 |
| PMID: | 38151568 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-072X |
|---|---|
| DOI: | 10.1007/s00203-023-03779-3 |