دورية أكاديمية
Structural and pharmacological characterization of a medium-chain fatty acid receptor GPR84 in common carp (Cyprinus carpio).
| العنوان: | Structural and pharmacological characterization of a medium-chain fatty acid receptor GPR84 in common carp (Cyprinus carpio). |
|---|---|
| المؤلفون: | Yao MX; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China., Yu HX; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China., Mo HL; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China., Zhang ZH; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China., Song QC; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China., Liu Q; Department of Pathology, The Second Affiliated Hospital of Air Force Medical University, Xi'an, Shaanxi, 710038, China., Yang QY; Department of Molecular, Cell and Cancer Biology, University of Massachusetts, Medical School, Worcester, MA, 01605, USA., Wang LX; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China., Li Y; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address: yangli2001@nwsuaf.edu.cn. |
| المصدر: | Developmental and comparative immunology [Dev Comp Immunol] 2024 Apr; Vol. 153, pp. 105126. Date of Electronic Publication: 2023 Dec 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004. |
| مواضيع طبية MeSH: | Carps*/genetics , Carps*/metabolism, Animals ; Phylogeny ; Fatty Acids/metabolism ; Decanoic Acids ; Lauric Acids ; Mammals |
| مستخلص: | The medium-chain fatty acid receptor GPR84, a member of the G protein-coupled receptor family, is mainly expressed in macrophages and microglia, and is involved in the regulation of inflammatory responses and retinal development in mammals and amphibians. However, structure, tissue distribution, and pharmacology of this receptor have rarely been reported in fish. In this study, we cloned the coding sequence (CDS) of common carp GPR84 (ccGPR84), examined its tissue distribution, and explored its cellular signaling function. The results showed that the CDS of ccGPR84 is 1191 bp and encodes a putative protein with 396 amino acids. Phylogenetic and chromosomal synteny analyses revealed that ccGPR84 was evolutionarily conserved with Cyprinids. Real-time quantitative PCR (qPCR) indicated that ccGPR84 was predominantly expressed in the intestine and spleen. Luciferase reporter assay demonstrated that nonanoic acid, capric acid (decanoic acid), undecanoic acid and lauric acid could inhibit cAMP signaling pathway and activate MAPK/ERK signaling pathway, while the potencies of these four fatty acids on the two signaling pathways were different. Lauric acid has the highest inhibitory potency on cAMP signaling pathway, followed by undecanoic acid, nonanoic acid, and capric acid. While for MAPK/ERK signaling pathway, nonanoic acid has the highest activation potency, followed by undecanoic acid, capric acid, and lauric acid. These findings lay the foundation for revealing the roles of different medium-chain fatty acids in the inflammatory response of common carp. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Common carp; GPR84; MAPK/ERK signaling pathway; cAMP signaling pathway |
| المشرفين على المادة: | 0 (Fatty Acids) 0 (Decanoic Acids) 0 (Lauric Acids) |
| تواريخ الأحداث: | Date Created: 20231231 Date Completed: 20240214 Latest Revision: 20240214 |
| رمز التحديث: | 20240214 |
| DOI: | 10.1016/j.dci.2023.105126 |
| PMID: | 38160872 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0089 |
|---|---|
| DOI: | 10.1016/j.dci.2023.105126 |