دورية أكاديمية
The Ability of a 3-Gene Host Signature in Blood to Distinguish Tuberculous Meningitis From Other Brain Infections.
| العنوان: | The Ability of a 3-Gene Host Signature in Blood to Distinguish Tuberculous Meningitis From Other Brain Infections. |
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| المؤلفون: | Huynh J; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford University, OxfordUnited Kingdom., Nhat LHT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Bao NLH; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Hai HT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Thu DDA; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Tram TTB; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Dung VTM; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Vinh DD; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Ngoc NM; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Donovan J; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford University, OxfordUnited Kingdom., Phu NH; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; School of Medicine, Vietnam National University of Ho Chi Minh City, Ho Chi Minh City, Vietnam., Van Thanh D; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Thu NTA; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Bang ND; Pham Ngoc Thach Hospital for Tuberculosis and Lung Disease, Ho Chi Minh City, Vietnam., Ha DTM; Pham Ngoc Thach Hospital for Tuberculosis and Lung Disease, Ho Chi Minh City, Vietnam., Nghia HDT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam.; The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam., Van Tan L; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford University, OxfordUnited Kingdom., Van LH; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Thwaites G; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford University, OxfordUnited Kingdom., Thuong NTT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Oxford University, OxfordUnited Kingdom. |
| المصدر: | The Journal of infectious diseases [J Infect Dis] 2024 Aug 16; Vol. 230 (2), pp. e268-e278. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print Cited Medium: Internet ISSN: 1537-6613 (Electronic) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press |
| مواضيع طبية MeSH: | Tuberculosis, Meningeal*/diagnosis , Tuberculosis, Meningeal*/blood , Tuberculosis, Meningeal*/genetics, Humans ; Male ; Female ; Adult ; Middle Aged ; GTP-Binding Proteins/genetics ; Kruppel-Like Transcription Factors/genetics ; Diagnosis, Differential ; Tuberculosis, Pulmonary/diagnosis ; Tuberculosis, Pulmonary/genetics ; Tuberculosis, Pulmonary/blood ; Biomarkers/blood ; Young Adult ; Vietnam ; ROC Curve |
| مستخلص: | Background: Tuberculous meningitis (TBM) is difficult to diagnose. We investigated whether a 3-gene host response signature in blood can distinguish TBM from other brain infections. Methods: The expression of 3 genes (dual specificity phosphatase 3 [DUSP3], guanylate-binding protein [GBP5], krupple-like factor 2 [KLF2]) was analyzed by RNA sequencing of archived whole blood from 4 cohorts of Vietnamese adults: 281 with TBM, 279 with pulmonary tuberculosis, 50 with other brain infections, and 30 healthy controls. Tuberculosis scores (combined 3-gene expression) were calculated following published methodology and discriminatory performance compared using area under a receiver operator characteristic curve (AUC). Results: GBP5 was upregulated in TBM compared to other brain infections (P < .001), with no difference in DUSP3 and KLF2 expression. The diagnostic performance of GBP5 alone (AUC, 0.74; 95% confidence interval [CI], .67-.81) was slightly better than the 3-gene tuberculosis score (AUC, 0.66; 95% CI, .58-.73) in TBM. Both GBP5 expression and tuberculosis score were higher in participants with human immunodeficiency virus (HIV; P < .001), with good diagnostic performance of GBP5 alone (AUC, 0.86; 95% CI, .80-.93). Conclusions: The 3-gene host signature in whole blood has the ability to discriminate TBM from other brain infections, including in individuals with HIV. Validation in large prospective diagnostic study is now required. Competing Interests: Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed. (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.) |
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| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust; Wellcome Trust Investigator; 206724/Z/17/Z United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: 3-gene host response; HIV coinfection; brain infections; diagnosis; tuberculous meningitis |
| المشرفين على المادة: | 0 (GBP5 protein, human) EC 3.6.1.- (GTP-Binding Proteins) 0 (Kruppel-Like Transcription Factors) 0 (Biomarkers) |
| تواريخ الأحداث: | Date Created: 20240103 Date Completed: 20240815 Latest Revision: 20240817 |
| رمز التحديث: | 20240817 |
| مُعرف محوري في PubMed: | PMC11326836 |
| DOI: | 10.1093/infdis/jiad606 |
| PMID: | 38169323 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-6613 |
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| DOI: | 10.1093/infdis/jiad606 |