دورية أكاديمية
The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch.
العنوان: | The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. |
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المؤلفون: | Photenhauer AL; Department of Microbiology & Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Villafuerte-Vega RC; Department of Chemistry, University of Michigan, Ann Arbor, MI, USA., Cerqueira FM; Department of Microbiology & Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Armbruster KM; Department of Microbiology & Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Mareček F; Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia., Chen T; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA., Wawrzak Z; Northwestern Synchrotron Research Center-LS-CAT, Northwestern University, Argonne, IL, USA., Hopkins JB; The Biophysics Collaborative Access Team (BioCAT), Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, IL, USA., Vander Kooi CW; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA., Janeček Š; Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia., Ruotolo BT; Department of Chemistry, University of Michigan, Ann Arbor, MI, USA., Koropatkin NM; Department of Microbiology & Immunology, University of Michigan Medical School, Ann Arbor, MI, USA. nkoropat@umich.edu. |
المصدر: | Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Feb; Vol. 31 (2), pp. 255-265. Date of Electronic Publication: 2024 Jan 04. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101186374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1545-9985 (Electronic) Linking ISSN: 15459985 NLM ISO Abbreviation: Nat Struct Mol Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: New York : Nature Pub. Group, c2004- |
مواضيع طبية MeSH: | Starch*/chemistry , Starch*/metabolism , Glucans*/chemistry , Glucans*/metabolism, Humans ; Amylopectin/metabolism ; Ruminococcus/metabolism ; Bacteria/metabolism |
مستخلص: | Resistant starch is a prebiotic accessed by gut bacteria with specialized amylases and starch-binding proteins. The human gut symbiont Ruminococcus bromii expresses Sas6 (Starch Adherence System member 6), which consists of two starch-specific carbohydrate-binding modules from family 26 (RbCBM26) and family 74 (RbCBM74). Here, we present the crystal structures of Sas6 and of RbCBM74 bound with a double helical dimer of maltodecaose. The RbCBM74 starch-binding groove complements the double helical α-glucan geometry of amylopectin, suggesting that this module selects this feature in starch granules. Isothermal titration calorimetry and native mass spectrometry demonstrate that RbCBM74 recognizes longer single and double helical α-glucans, while RbCBM26 binds short maltooligosaccharides. Bioinformatic analysis supports the conservation of the amylopectin-targeting platform in CBM74s from resistant-starch degrading bacteria. Our results suggest that RbCBM74 and RbCBM26 within Sas6 recognize discrete aspects of the starch granule, providing molecular insight into how this structure is accommodated by gut bacteria. (© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.) |
References: | Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):582-601. (PMID: 19465773) Appl Environ Microbiol. 2014 Oct;80(19):6080-90. (PMID: 25063659) J Appl Crystallogr. 2017 Sep 05;50(Pt 5):1545-1553. (PMID: 29021737) Methods Mol Biol. 2020;2112:29-42. (PMID: 32006276) Chemotherapy. 1995;41 Suppl 1:5-15. (PMID: 7671648) Acta Crystallogr D Struct Biol. 2017 Mar 1;73(Pt 3):223-233. (PMID: 28291757) Commun Biol. 2022 Sep 8;5(1):932. (PMID: 36076058) Protein Sci. 2020 Jan;29(1):66-75. (PMID: 31576635) Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) Appl Environ Microbiol. 2007 Jun;73(12):3833-7. (PMID: 17468268) Protein Sci. 2020 Jan;29(1):128-140. (PMID: 31606894) Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887) Nucleic Acids Res. 2016 Jul 8;44(W1):W344-50. (PMID: 27166375) Methods Enzymol. 2012;510:211-31. (PMID: 22608728) Curr Opin Struct Biol. 2013 Oct;23(5):686-94. (PMID: 24080387) Microbiome. 2016 Jun 29;4(1):33. (PMID: 27357127) Annu Rev Biochem. 2010;79:655-81. (PMID: 20373916) Crit Rev Biotechnol. 2016;36(3):578-84. (PMID: 25582732) Mol Syst Biol. 2011 Oct 11;7:539. (PMID: 21988835) Nat Struct Mol Biol. 2015 Nov;22(11):833-4. (PMID: 26581513) Environ Microbiol. 2018 Jan;20(1):324-336. (PMID: 29159997) Trends Biotechnol. 1994 Sep;12(9):379-86. (PMID: 7765191) Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4246-51. (PMID: 10200247) J Biol Chem. 2022 May;298(5):101896. (PMID: 35378131) ISME J. 2012 Aug;6(8):1535-43. (PMID: 22343308) J Am Chem Soc. 2018 Jun 20;140(24):7486-7497. (PMID: 29733584) Nucleic Acids Res. 2021 Jan 8;49(D1):D344-D354. (PMID: 33156333) Anal Chem. 2021 Mar 9;93(9):4231-4239. (PMID: 33630563) mBio. 2015 Sep 29;6(5):e01058-15. (PMID: 26419877) Anal Chem. 2003 Oct 1;75(19):4945-55. (PMID: 14708765) Biochemistry. 2001 May 29;40(21):6248-56. (PMID: 11371186) J Microbiol Biotechnol. 2019 Dec 28;29(12):1904-1915. (PMID: 31635446) Nat Protoc. 2014 Jan;9(1):156-70. (PMID: 24356774) Nat Methods. 2017 Mar;14(3):283-286. (PMID: 28114288) Anal Chem. 2015 Jun 2;87(11):5738-44. (PMID: 25929282) Int J Biol Macromol. 2020 Oct 15;161:389-397. (PMID: 32479932) J Biol Chem. 2006 Jan 6;281(1):587-98. (PMID: 16230347) Trends Microbiol. 2020 Feb;28(2):95-108. (PMID: 31624005) Biophys J. 2018 Jun 5;114(11):2485-2492. (PMID: 29874600) J Biol Chem. 2022 Jul;298(7):102089. (PMID: 35640720) mBio. 2019 Jan 29;10(1):. (PMID: 30696735) J Ind Microbiol Biotechnol. 2009 Mar;36(3):341-6. (PMID: 19052787) Anal Chem. 2015 Apr 21;87(8):4370-6. (PMID: 25799115) J Am Chem Soc. 2003 Dec 3;125(48):14859-66. (PMID: 14640663) Adv Neurobiol. 2019;23:17-81. (PMID: 31667805) Biotechnol Adv. 2019 Dec;37(8):107451. (PMID: 31536775) Gut Microbes. 2021 Jan-Dec;13(1):1926842. (PMID: 34275431) Science. 2005 Mar 25;307(5717):1915-20. (PMID: 15790844) J Mol Biol. 2007 Sep 21;372(3):774-97. (PMID: 17681537) Acta Crystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2197-203. (PMID: 25084338) Bioinformatics. 2007 Jan 1;23(1):127-8. (PMID: 17050570) Microbiol Mol Biol Rev. 2005 Jun;69(2):306-25. (PMID: 15944458) Protein Sci. 2019 Feb;28(2):454-463. (PMID: 30371978) J Nutr. 1995 Jun;125(6):1401-12. (PMID: 7782892) Mol Biol Evol. 2001 May;18(5):691-9. (PMID: 11319253) Appl Environ Microbiol. 2015 Oct;81(19):6610-20. (PMID: 26187958) J Mol Biol. 1988 May 20;201(2):365-78. (PMID: 3418703) Nucleic Acids Res. 2010 Jul;38(Web Server issue):W529-33. (PMID: 20478830) Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67. (PMID: 22505256) Anim Microbiome. 2021 Jul 3;3(1):45. (PMID: 34217374) Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765) J Appl Crystallogr. 2021 Feb 01;54(Pt 1):343-355. (PMID: 33833657) mBio. 2016 Apr 26;7(2):e02134-15. (PMID: 27118585) Nucleic Acids Res. 2022 Jan 7;50(D1):D571-D577. (PMID: 34850161) Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32. (PMID: 20124692) Appl Environ Microbiol. 2006 May;72(5):3593-9. (PMID: 16672507) Front Microbiol. 2021 Apr 29;12:640253. (PMID: 33995299) Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):293-302. (PMID: 21460447) Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14. (PMID: 23793146) Nature. 2013 Apr 25;496(7446):477-81. (PMID: 23619693) J Cancer. 2018 Jun 15;9(14):2510-2517. (PMID: 30026849) Carbohydr Polym. 2008 Sep 5;73(4):515-31. (PMID: 26048217) Evolution. 1985 Jul;39(4):783-791. (PMID: 28561359) Nucleic Acids Res. 2021 Jan 8;49(D1):D480-D489. (PMID: 33237286) Biopolymers. 1999 Oct 5;50(4):381-90. (PMID: 10423547) J Appl Bacteriol. 1991 Jun;70(6):443-59. (PMID: 1938669) J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674. (PMID: 19461840) Nat Commun. 2018 Nov 12;9(1):4764. (PMID: 30420680) Acta Crystallogr D Struct Biol. 2016 Dec 1;72(Pt 12):1254-1266. (PMID: 27917826) Nucleic Acids Res. 2016 Jul 8;44(W1):W424-9. (PMID: 27151198) J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712) Mol Microbiol. 2018 Jan;107(2):249-264. (PMID: 29139580) FEBS J. 2016 Jun;283(12):2354-68. (PMID: 27101946) Phys Chem Chem Phys. 2013 Jun 21;15(23):8952-61. (PMID: 23612608) Nucleic Acids Res. 2021 Jan 8;49(D1):D92-D96. (PMID: 33196830) Nucleic Acids Res. 2018 Jul 2;46(W1):W95-W101. (PMID: 29771380) Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5. (PMID: 24270786) |
معلومات مُعتمدة: | S10 OD018090 United States OD NIH HHS; P01 HL149633 United States HL NHLBI NIH HHS; R01 GM095832 United States GM NIGMS NIH HHS; P30 GM138395 United States GM NIGMS NIH HHS; F31 AT011282 United States AT NCCIH NIH HHS |
المشرفين على المادة: | 9005-25-8 (Starch) 0 (Glucans) 9037-22-3 (Amylopectin) |
SCR Organism: | Ruminococcus bromii |
تواريخ الأحداث: | Date Created: 20240104 Date Completed: 20240219 Latest Revision: 20240511 |
رمز التحديث: | 20240511 |
مُعرف محوري في PubMed: | PMC11081458 |
DOI: | 10.1038/s41594-023-01166-6 |
PMID: | 38177679 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1545-9985 |
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DOI: | 10.1038/s41594-023-01166-6 |