دورية أكاديمية
MicroRNA-195-5p Attenuates Pregnancy-Induced Hypertension by Inhibiting Oxidative Stress via OTX1/MAPK Signaling Pathway.
| العنوان: | MicroRNA-195-5p Attenuates Pregnancy-Induced Hypertension by Inhibiting Oxidative Stress via OTX1/MAPK Signaling Pathway. |
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| المؤلفون: | Liu L; Hospital Infection Management Division, Maternal and Child Health Hospital of Tangshan, Tangshan, 063000, China., Yao C; Department of Obstetrics Gynecology, Tangshan Workers' Hospital, Tangshan, 063000, China., Song Z; Department of Gynecology and Obstetrics, Maternal and Child Health Hospital of Tangshan, No. 1 Hetai Road, Lunan District, Tangshan, 063000, China. songzhdoctor@hotmail.com. |
| المصدر: | Biochemical genetics [Biochem Genet] 2024 Oct; Vol. 62 (5), pp. 3642-3657. Date of Electronic Publication: 2024 Jan 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 0126611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4927 (Electronic) Linking ISSN: 00062928 NLM ISO Abbreviation: Biochem Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : New York : Kluwer Academic/Plenum Publishers Original Publication: New York, Plenum Press. |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Hypertension, Pregnancy-Induced*/metabolism , Hypertension, Pregnancy-Induced*/genetics , Oxidative Stress* , Otx Transcription Factors*/metabolism , Otx Transcription Factors*/genetics , MAP Kinase Signaling System*, Animals ; Female ; Pregnancy ; Rats ; Humans ; Rats, Sprague-Dawley ; Placenta/metabolism ; Disease Models, Animal |
| مستخلص: | Pregnancy-induced hypertension (PIH) is a hypertensive disorder during pregnancy and can induce perinatal death of human infants. MicroRNA (miR)-195-5p was validated to display low expression in severe preeclampsia placentas, but the role of miR-195-5p in pregnancy-induced hypertension (PIH) has not been investigated. The study emphasized on the functions and mechanism of miR-195-5p in PIH. A reduced uterine perfusion pressure (RUPP) rat model was established to mimic PIH in vivo. Adenovirus (Ad)-miR-195-5p agomir and/or Ad-OTX1 were further injected into some model rats. RT-qPCR was conducted to assess the expression of miR-195-5p and orthodenticle homeobox 1 (OTX1) in rat placental tissues, the isolated aortic endothelial cells (AECs), and in serum samples of PIH patients. Western blot analysis was implemented to measure the protein levels of OTX1, VEGFA, and key factors involved in the MAPK signaling pathway. The concentrations of oxidative stress markers (superoxide dismutase, catalase, and lipid hydroperoxide) in AECs and placental tissues of RUPP rats were measured by corresponding kits. The binding relation between miR-195-5p and OTX1 was verified using the dual-luciferase reporter assay. Hematoxylin-eosin staining was conducted to evaluate the pathological features of rat placental tissues. MiR-195-5p was downregulated, while OTX1 was upregulated in rat placental tissues and human serum samples of PIH patients. MiR-195-5p could target OTX1 and inversely regulate OTX1 expression in AECs and rat placental tissues. In addition, miR-195-5p can negatively regulate VEGFA level. Furthermore, miR-195-5p inactivates oxidative stress and the MAPK signaling by downregulating OTX1 in AECs. In vivo experiments revealed that OTX1 overexpression reversed the protective effect of miR-195-5p overexpression on placental damage and oxidative stress. MiR-195-5p alleviates PIH by inhibiting oxidative stress via targeting OTX1 and inactivating MAPK signaling. (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Acunzo M et al (2015) MicroRNA and cancer—a brief overview. Adv Biol Regul 57:1–9. (PMID: 10.1016/j.jbior.2014.09.01325294678) Awamleh Z, Han VKM (2020) Identification of miR-210-5p in human placentae from pregnancies complicated by preeclampsia and intrauterine growth restriction, and its potential role in the pregnancy complications. Pregnancy Hypertens 19:159–168. (PMID: 10.1016/j.preghy.2020.01.00232014817) Bai J et al (2023) Chlorpyrifos induces placental oxidative stress and barrier dysfunction by inducing mitochondrial apoptosis through the ERK/MAPK signaling pathway: in vitro and in vivo studies. Sci Total Environ 903:166449. (PMID: 10.1016/j.scitotenv.2023.16644937634732) Bartel DP (2018) Metazoan MicroRNAs. Cell 173(1):20–51. (PMID: 10.1016/j.cell.2018.03.006295709946091663) Chen FY et al (2020) Long non-coding RNA MIR100HG promotes the migration, invasion and proliferation of triple-negative breast cancer cells by targeting the miR-5590-3p/OTX1 axis. Cancer Cell Int 20:508. (PMID: 10.1186/s12935-020-01580-6330882167568413) Chiarello DI et al (2020) Oxidative stress: Normal pregnancy versus preeclampsia. Biochim Biophys Acta Mol Basis Dis 1866(2):165354. (PMID: 10.1016/j.bbadis.2018.12.00530590104) Gao Z et al (2017) Molecular mechanism of miR-181b in heart disease due to pregnancy-induced hypertension syndrome. Exp Ther Med 14(4):2953–2959. (PMID: 10.3892/etm.2017.4882289128515585881) Ho PTB, Clark IM, Le LTT (2022) MicroRNA-based diagnosis and therapy. Int J Mol Sci 23(13):1215. (PMID: 10.3390/ijms23137167) Hu Y et al (2021) Serum miR-195-5p exhibits clinical significance in the diagnosis of essential hypertension with type 2 diabetes mellitus by targeting DRD1. Clinics (sao Paulo) 76:e2502. (PMID: 10.6061/clinics/2021/e250234495077) Hu S et al (2023) MiR-195-5p suppresses gastric adenocarcinoma cell progression via targeting OTX1. Histol Histopathol 38(6):659–668. (PMID: 36093844) Jena MK et al (2020) Pathogenesis of preeclampsia and therapeutic approaches targeting the placenta. Biomolecules 10(6):953. (PMID: 10.3390/biom10060953325998567357118) Kintiraki E et al (2015) Pregnancy-induced hypertension. Hormones (athens) 14(2):211–223. (PMID: 10.14310/horm.2002.158226158653) Kobayashi M et al (2005) A simple method of isolating mouse aortic endothelial cells. J Atheroscler Thromb 12(3):138–142. (PMID: 10.5551/jat.12.13816020913) Larsen KB et al (2010) Expression of the homeobox genes OTX2 and OTX1 in the early developing human brain. J Histochem Cytochem 58(7):669–678. (PMID: 10.1369/jhc.2010.955757203541452889402) Li H et al (2016) OTX1 contributes to hepatocellular carcinoma progression by regulation of ERK/MAPK pathway. J Korean Med Sci 31(8):1215–1223. (PMID: 10.3346/jkms.2016.31.8.1215274783314951550) Li J, Wu Y, Liu H (2020) Expression and role of miR-338-3p in peripheral blood and placenta of patients with pregnancy-induced hypertension. Exp Ther Med 20(1):418–426. (PMID: 10.3892/etm.2020.8719325370067282187) Li JY et al (2022a) MiR-140-5p exerts a protective function in pregnancy-induced hypertension via mediating TGF-β/Smad signaling pathway. Hypertens Pregnancy 41(2):116–125. (PMID: 10.1080/10641955.2022.205619535354421) Li K et al (2022b) CircFN1 upregulation initiated oxidative stress-induced apoptosis and inhibition of proliferation and migration in trophoblasts via circFN1-miR-19a/b-3p-ATF2 ceRNA network. Reprod Biol 22(2):100631. (PMID: 10.1016/j.repbio.2022.10063135259622) Liu M et al (2021) Atractylenolide inhibits apoptosis and oxidative stress of HTR-8/SVneo cells by activating MAPK/ERK signalling in preeclampsia. Phytomedicine 93:153773. (PMID: 10.1016/j.phymed.2021.15377334649213) Liu Z, Guo N, Zhang XJ (2022) Long noncoding TUG1 promotes angiogenesis of HUVECs in PE via regulating the miR-29a-3p/VEGFA and Ang2/Tie2 pathways. Microvasc Res 139:104231. (PMID: 10.1016/j.mvr.2021.10423134352236) Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402–408. (PMID: 10.1006/meth.2001.126211846609) Luo JY et al (2016) Inhibition of the JAK2/STAT3/SOSC1 signaling pathway improves secretion function of vascular endothelial cells in a rat model of pregnancy-induced hypertension. Cell Physiol Biochem 40(3–4):527–537. (PMID: 10.1159/00045256627889763) Magee LA, von Dadelszen P (2018) State-of-the-art diagnosis and treatment of hypertension in pregnancy. Mayo Clin Proc 93(11):1664–1677. (PMID: 10.1016/j.mayocp.2018.04.03330392546) Mazzuca MQ et al (2014) Downregulation of microvascular endothelial type B endothelin receptor is a central vascular mechanism in hypertensive pregnancy. Hypertension 64(3):632–643. (PMID: 10.1161/HYPERTENSIONAHA.114.0331524914193) McGeary SE et al (2019) The biochemical basis of microRNA targeting efficacy. Science. https://doi.org/10.1126/science.aav1741. (PMID: 10.1126/science.aav1741318066987051167) Ou M et al (2014) Adaptive regulation of endothelin receptor type-A and type-B in vascular smooth muscle cells during pregnancy in rats. J Cell Physiol 229(4):489–501. (PMID: 10.1002/jcp.2446924105843) Ou M et al (2020) Long noncoding RNA MALAT1 contributes to pregnancy-induced hypertension development by enhancing oxidative stress and inflammation through the regulation of the miR-150-5p/ET-1 axis. Faseb j 34(5):6070–6085. (PMID: 10.1096/fj.201902280R32246794) Phoswa WN, Khaliq OP (2021) The role of oxidative stress in hypertensive disorders of pregnancy (preeclampsia, gestational hypertension) and metabolic disorder of pregnancy (gestational diabetes mellitus). Oxid Med Cell Longev 2021:5581570. (PMID: 10.1155/2021/5581570341946068184326) Rao ZZ et al (2017) miR-148a-mediated estrogen-induced cholestasis in intrahepatic cholestasis of pregnancy: role of PXR/MRP3. PLoS ONE 12(6):e0178702. (PMID: 10.1371/journal.pone.0178702285750985457162) Report of the national high blood pressure education program working group on high blood pressure in pregnancy. Am J Obstet Gynecol, 2000. 183(1): S1–s22. Sandrim VC et al (2016) Plasma from pre-eclamptic patients induces the expression of the anti-angiogenic miR-195-5p in endothelial cells. J Cell Mol Med 20(6):1198–1200. (PMID: 10.1111/jcmm.12767270129264882987) Tamargo J, Caballero R, Delpón E (2019) Pharmacotherapy for hypertension in pregnant patients: special considerations. Expert Opin Pharmacother 20(8):963–982. (PMID: 10.1080/14656566.2019.159477330943045) Tang F et al (2022) lncRNA H19 promotes Ox-LDL-induced dysfunction of human aortic endothelial cells through the miR-152/VEGFA axis. J Healthc Eng 2022:3795060. (PMID: 10.1155/2022/3795060353456608957438) Toboła-Wróbel K et al (2020) Association of oxidative stress on pregnancy. Oxid Med Cell Longev 2020:6398520. (PMID: 10.1155/2020/6398520330142747512072) Van de Vusse D et al (2022) Pharmacokinetics of the most commonly used antihypertensive drugs throughout pregnancy methyldopa, labetalol, and nifedipine: a systematic review. Eur J Clin Pharmacol 78(11):1763–1776. https://doi.org/10.1007/s00228-022-03382-3. (PMID: 10.1007/s00228-022-03382-3361044509474278) Wang P et al (2019) Dysregulation of microRNA-657 influences inflammatory response via targeting interleukin-37 in gestational diabetes mellitus. J Cell Physiol 234(5):7141–7148. (PMID: 10.1002/jcp.2746830362558) Xu P et al (2014) Variations of microRNAs in human placentas and plasma from preeclamptic pregnancy. Hypertension 63(6):1276–1284. (PMID: 10.1161/HYPERTENSIONAHA.113.0264724664294) Xu Y et al (2020) miR-195-5p alleviates acute kidney injury through repression of inflammation and oxidative stress by targeting vascular endothelial growth factor A. Aging 12(11):10235–10245. (PMID: 10.18632/aging.103160324926577346085) Yuan Y et al (2020) MicroRNA-16 is involved in the pathogenesis of pre-eclampsia via regulation of Notch2. J Cell Physiol 235(5):4530–4544. (PMID: 10.1002/jcp.2933031643078) Zeng Z et al (2018) Anti-apoptosis endothelial cell-secreted microRNA-195-5p promotes pulmonary arterial smooth muscle cell proliferation and migration in pulmonary arterial hypertension. J Cell Biochem 119(2):2144–2155. (PMID: 10.1002/jcb.2637628862358) Zhang H et al (2020) miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia. Redox Biol 29:101402. (PMID: 10.1016/j.redox.2019.10140231926626) |
| فهرسة مساهمة: | Keywords: OTX1; Oxidative stress; Pregnancy-induced hypertension; Reduced uterine perfusion pressure; miR-195-5p |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Otx Transcription Factors) 0 (MIRN195 microRNA, human) 0 (OTX1 protein, human) 0 (MIRN195 microRNA, rat) |
| تواريخ الأحداث: | Date Created: 20240104 Date Completed: 20240926 Latest Revision: 20240926 |
| رمز التحديث: | 20240927 |
| DOI: | 10.1007/s10528-023-10612-5 |
| PMID: | 38177835 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1573-4927 |
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| DOI: | 10.1007/s10528-023-10612-5 |