دورية أكاديمية
Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139.
| العنوان: | Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139. |
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| المؤلفون: | Saha M; Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology (CSIR), Kolkata-700032, India., Pragasam AK; Centre for Microbial Research, Translational Health Science and Technology Institute, Faridabad-121001, Haryana, India., Kumari S; Centre for Microbial Research, Translational Health Science and Technology Institute, Faridabad-121001, Haryana, India., Verma J; Centre for Microbial Research, Translational Health Science and Technology Institute, Faridabad-121001, Haryana, India., Das B; Centre for Microbial Research, Translational Health Science and Technology Institute, Faridabad-121001, Haryana, India., Bhadra RK; Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology (CSIR), Kolkata-700032, India. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2024 Jan; Vol. 170 (1). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print Cited Medium: Internet ISSN: 1465-2080 (Electronic) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Vibrio cholerae non-O1*, Anti-Bacterial Agents/pharmacology ; Genomics ; Chloramphenicol ; Streptomycin ; Drug Resistance, Microbial |
| مستخلص: | The emergence and spread of antibiotic-resistant bacterial pathogens are a critical public health concern across the globe. Mobile genetic elements (MGEs) play an important role in the horizontal acquisition of antimicrobial resistance genes (ARGs) in bacteria. In this study, we have decoded the whole genome sequences of multidrug-resistant Vibrio cholerae clinical isolates carrying the ARG-linked SXT, an integrative and conjugative element, in their large chromosomes. As in others, the SXT element has been found integrated into the 5'-end of the prfC gene (which encodes peptide chain release factor 3 involved in translational regulation) on the large chromosome of V. cholerae non-O1/non-O139 strains. Further, we demonstrate the functionality of SXT-linked floR and strAB genes, which confer resistance to chloramphenicol and streptomycin, respectively. The floR gene-encoded protein FloR belongs to the major facilitator superfamily efflux transporter containing 12 transmembrane domains (TMDs). Deletion analysis confirmed that even a single TMD of FloR is critical for the export function of chloramphenicol. The floR gene has two putative promoters, P1 and P2. Sequential deletions reveal that P2 is responsible for the expression of the floR . Deletion analysis of the N- and/or C-terminal coding regions of strA established their importance for conferring resistance against streptomycin. Interestingly, qPCR analysis of the floR and strA genes indicated that both of the genes are constitutively expressed in V. cholerae cells. Further, whole genome-based global phylogeography confirmed the presence of the integrative and conjugative element SXT in non-O1/non-O139 strains despite being non-multidrug resistant by lacking antimicrobial resistance (AMR) gene cassettes, which needs monitoring. |
| References: | BMC Microbiol. 2009 Dec 29;9:275. (PMID: 20040104) Mol Microbiol. 1999 Apr;32(1):99-110. (PMID: 10216863) J Bacteriol. 1996 Jul;178(14):4157-65. (PMID: 8763944) Microbiology (Reading). 2017 Jun;163(6):900-910. (PMID: 28597815) Infect Immun. 2008 Aug;76(8):3595-605. (PMID: 18490456) Nucleic Acids Res. 2019 Jul 2;47(W1):W256-W259. (PMID: 30931475) Drugs. 2004;64(2):159-204. (PMID: 14717618) Genome Biol. 2020 Jul 22;21(1):180. (PMID: 32698896) J Bacteriol. 1979 Sep;139(3):877-82. (PMID: 113388) Infect Immun. 1999 Jan;67(1):148-54. (PMID: 9864209) FEMS Microbiol Lett. 1999 May 15;174(2):327-32. (PMID: 10339826) J Appl Microbiol. 2002;92 Suppl:65S-71S. (PMID: 12000614) mBio. 2016 Jul 19;7(4):. (PMID: 27435459) J Bacteriol. 2003 Aug;185(16):4672-82. (PMID: 12896985) PLoS One. 2017 Jan 19;12(1):e0169324. (PMID: 28103259) Bioinformatics. 2014 May 1;30(9):1312-3. (PMID: 24451623) FEMS Microbiol Rev. 2004 Nov;28(5):519-42. (PMID: 15539072) Vet Res. 2001 May-Aug;32(3-4):301-10. (PMID: 11432421) J Bacteriol. 2008 Jul;190(13):4764-71. (PMID: 18456812) Arch Microbiol. 2008 Mar;189(3):227-38. (PMID: 17968531) Antimicrob Agents Chemother. 1981 Feb;19(2):294-7. (PMID: 6957162) PLoS One. 2014 Sep 29;9(9):e108728. (PMID: 25265418) J Med Microbiol. 2007 Mar;56(Pt 3):346-351. (PMID: 17314365) J Antimicrob Chemother. 2018 Jan 01;73(1):57-65. (PMID: 29029160) Nat Commun. 2022 Jul 5;13(1):3864. (PMID: 35790755) J Bacteriol. 2001 Feb;183(4):1124-32. (PMID: 11157923) Genomics. 2021 Nov;113(6):3951-3966. (PMID: 34619341) Genom Discov. 2014;2(1):1-15. (PMID: 25722857) J Antimicrob Chemother. 2004 Jul;54(1):286-8. (PMID: 15175268) FEMS Microbiol Lett. 2002 Apr 9;209(2):209-13. (PMID: 12007807) Clin Microbiol Rev. 1995 Jan;8(1):48-86. (PMID: 7704895) Mol Microbiol. 2009 Apr;72(2):380-98. (PMID: 19298370) Mol Microbiol. 2007 Feb;63(3):848-58. (PMID: 17181782) Front Cell Infect Microbiol. 2020 Jul 31;10:369. (PMID: 32903722) Emerg Infect Dis. 2011 Nov;17(11):2151-4. (PMID: 22099122) Environ Microbiol. 2008 Apr;10(4):866-73. (PMID: 18201198) J Bacteriol. 2012 Oct;194(20):5638-48. (PMID: 22904284) Genome Res. 2015 Jul;25(7):1043-55. (PMID: 25977477) Microbiology (Reading). 2019 Jan;165(1):102-112. (PMID: 30444469) Plasmid. 2006 May;55(3):173-83. (PMID: 16530834) Sci Rep. 2016 Mar 09;6:22733. (PMID: 26956038) Antimicrob Agents Chemother. 2005 Jul;49(7):2965-71. (PMID: 15980376) J Antimicrob Chemother. 2009 Mar;63(3):438-42. (PMID: 19155226) Appl Environ Microbiol. 2003 Apr;69(4):2389-94. (PMID: 12676727) Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063) Cell Mol Life Sci. 2002 Dec;59(12):2065-70. (PMID: 12568332) |
| فهرسة مساهمة: | Keywords: SXT; antibiotic resistance; cholera; genome |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 66974FR9Q1 (Chloramphenicol) Y45QSO73OB (Streptomycin) |
| تواريخ الأحداث: | Date Created: 20240105 Date Completed: 20240108 Latest Revision: 20240417 |
| رمز التحديث: | 20240417 |
| مُعرف محوري في PubMed: | PMC10866021 |
| DOI: | 10.1099/mic.0.001424 |
| PMID: | 38180462 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2080 |
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| DOI: | 10.1099/mic.0.001424 |