دورية أكاديمية
أعرض في EDS

Gene profiling in active dermatitis lesions strengthens the diagnosis of allergic contact dermatitis.

التفاصيل البيبلوغرافية
العنوان: Gene profiling in active dermatitis lesions strengthens the diagnosis of allergic contact dermatitis.
المؤلفون: Lefevre MA; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France; Department of Dermatology and Allergy, Centre Hospitalier Universitaire de St Etienne, Saint-Priest-en-Jarez, France. Electronic address: marine-alexia.lefevre@inserm.fr., Nosbaum A; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France; Department of Allergy and Clinical Immunology, Centre Hospitalier Lyon-Sud, Pierre-Benite, France., Mosnier A; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France., Lenief V; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France., Salque S; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France., Pichot M; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France., Maheux L; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France., Bertolotti L; Department of Allergy and Clinical Immunology, Centre Hospitalier Lyon-Sud, Pierre-Benite, France., Hacard F; Department of Allergy and Clinical Immunology, Centre Hospitalier Lyon-Sud, Pierre-Benite, France., Graveriau C; Private Dermatology Practice, Lyon, France., Zukervar P; Private Dermatology Practice, Lyon, France., Breton Guitarian AL; Private Dermatology Practice, Lyon, France., Boisleve F; Chanel, Neuilly-sur-Seine, France., Elbaz M; Chanel, Neuilly-sur-Seine, France., Nicolas JF; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France; Department of Allergy and Clinical Immunology, Centre Hospitalier Lyon-Sud, Pierre-Benite, France., Vocanson M; CIRI, Centre International de Recherche en Infectiologie, Team Epidermal Immunity and Allergy, INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR, Lyon, France. Electronic address: marc.vocanson@inserm.fr.
المصدر: Journal of the American Academy of Dermatology [J Am Acad Dermatol] 2024 May; Vol. 90 (5), pp. 953-962. Date of Electronic Publication: 2024 Jan 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Mosby Country of Publication: United States NLM ID: 7907132 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-6787 (Electronic) Linking ISSN: 01909622 NLM ISO Abbreviation: J Am Acad Dermatol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: St. Louis, Mo., Mosby
مواضيع طبية MeSH: Dermatitis, Allergic Contact*/diagnosis , Dermatitis, Allergic Contact*/genetics , Dermatitis, Allergic Contact*/pathology , Dermatitis, Irritant*/diagnosis, Humans ; Irritants ; Allergens ; Patch Tests/methods ; Biomarkers
مستخلص: Background: Distinguishing between allergic and nonallergic forms of Contact Dermatitis (CD) is challenging and requires investigations based on patch-testing. Early detection of allergy biomarkers in active CD lesions could refine and simplify the management of CD patients.
Objective: To characterize the molecular signatures of active CD lesions.
Methods: We studied the expression of 12 allergy biomarkers by qRT-PCR in active lesions of 38 CD patients. Allergic CD (ACD) was diagnosed based on patch test (PT) results and exposure assessment. Molecular signatures of active lesions, as well as positive PT reactions, were compared with those of reference chemical allergens and irritants.
Results: Nineteen of the 38 CD patients reacted positively upon patch-testing and exposure assessment confirmed ACD diagnosis for 17 of them. Gene profiling of active CD lesions revealed 2 distinct molecular patterns: patients harboring signatures similar to reference allergens (n = 23) or irritants (n = 15). Among the 23 patients with an "allergy signature," we found the 17 patients with confirmed ACD, while no culprit allergen was identified for the 6 other patients. Interestingly, the 15 patients without biomarker induction had negative PT, suggesting that they developed nonallergic CD reactions.
Conclusion: Molecular signatures from active skin lesions may help to stratify CD patients and predict those suffering from ACD.
Competing Interests: Conflicts of interest None disclosed.
(Copyright © 2024 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: allergic contact dermatitis; biomarkers; irritant contact dermatitis; molecular signature; point of care diagnosis; transcriptomic profiling
المشرفين على المادة: 0 (Irritants)
0 (Allergens)
0 (Biomarkers)
تواريخ الأحداث: Date Created: 20240112 Date Completed: 20240415 Latest Revision: 20240415
رمز التحديث: 20240415
DOI: 10.1016/j.jaad.2023.11.066
PMID: 38215793
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-6787
DOI:10.1016/j.jaad.2023.11.066