دورية أكاديمية
Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?
| العنوان: | Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe? |
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| المؤلفون: | Arykbaeva AS; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., Lerink LJS; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., Vos J; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., Engelse MA; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK., van Kooten C; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK., de Korte D; Division of Nephrology and Transplant Medicine, Leiden University Medical Center, Leiden, The Netherlands., Lagerberg JWM; Division of Nephrology and Transplant Medicine, Leiden University Medical Center, Leiden, The Netherlands., Klei TRL; Division of Nephrology and Transplant Medicine, Leiden University Medical Center, Leiden, The Netherlands., Mulder AA; Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands., Minnee RC; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands., Ploeg RJ; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Department of Surgery, Division of Hepatopancreatobiliary and Transplant Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, The Netherlands., Kers J; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Department of Pathology, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands; Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, The Netherlands., Moers C; Department of Surgery - Organ Donation and Transplantation, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands., Pol RA; Department of Surgery - Organ Donation and Transplantation, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands., Alwayn IPJ; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., de Vries DK; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., Lindeman JHN; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: j.h.n.lindeman@lumc.nl. |
| المصدر: | American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons [Am J Transplant] 2024 Jul; Vol. 24 (7), pp. 1172-1179. Date of Electronic Publication: 2024 Jan 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 100968638 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-6143 (Electronic) Linking ISSN: 16006135 NLM ISO Abbreviation: Am J Transplant Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York] : Elsevier Original Publication: Copenhagen : Munksgaard International Publishers, 2001- |
| مواضيع طبية MeSH: | Perfusion* , Erythrocytes*/metabolism , Kidney Transplantation* , Organ Preservation*/methods , Oxygen*/metabolism, Humans ; Hemolysis ; Animals ; Male ; Kidney/metabolism |
| مستخلص: | Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: kidney transplantation/nephrology; normothermic machine perfusion; organ perfusion and preservation; oxygen carrier; red blood cells |
| المشرفين على المادة: | S88TT14065 (Oxygen) |
| تواريخ الأحداث: | Date Created: 20240112 Date Completed: 20240717 Latest Revision: 20240717 |
| رمز التحديث: | 20240718 |
| DOI: | 10.1016/j.ajt.2024.01.002 |
| PMID: | 38215981 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1600-6143 |
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| DOI: | 10.1016/j.ajt.2024.01.002 |