دورية أكاديمية
Profibrotic Subsets of SPP1 + Macrophages and POSTN + Fibroblasts Contribute to Fibrotic Scarring in Acne Keloidalis.
| العنوان: | Profibrotic Subsets of SPP1 + Macrophages and POSTN + Fibroblasts Contribute to Fibrotic Scarring in Acne Keloidalis. |
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| المؤلفون: | Hong YK; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, Taiwan., Hwang DY; National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan., Yang CC; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, Taiwan., Cheng SM; National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan., Chen PC; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Aala WJ; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan., I-Chen Harn H; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA., Evans ST; Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA., Onoufriadis A; St John's Institute of Dermatology, School of Basic & Medical Biosciences, King's College London, London, United Kingdom., Liu SL; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Lin YC; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, Taiwan., Chang YH; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Lo TK; Department of Dermatology, Tainan Municipal An-Nan Hospital, Tainan, Taiwan., Hung KS; Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Lee YC; PhD Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan., Tang MJ; International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, Taiwan; Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Lu KQ; Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA., McGrath JA; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; St John's Institute of Dermatology, School of Basic & Medical Biosciences, King's College London, London, United Kingdom., Hsu CK; Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, Taiwan; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address: kylehsu@mail.ncku.edu.tw. |
| المصدر: | The Journal of investigative dermatology [J Invest Dermatol] 2024 Jul; Vol. 144 (7), pp. 1491-1504.e10. Date of Electronic Publication: 2024 Jan 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0426720 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1747 (Electronic) Linking ISSN: 0022202X NLM ISO Abbreviation: J Invest Dermatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : New York : Elsevier Original Publication: Baltimore, Williams & Wilkins. |
| مواضيع طبية MeSH: | Fibroblasts*/metabolism , Fibroblasts*/pathology , Macrophages*/metabolism , Macrophages*/pathology , Acne Keloid*/pathology , Acne Keloid*/metabolism, Humans ; Osteopontin/metabolism ; Osteopontin/genetics ; Fibrosis ; Male ; Cell Adhesion Molecules/metabolism ; Cell Adhesion Molecules/genetics ; Female ; Adult ; Cicatrix/pathology ; Scalp/pathology ; Cell Communication ; Biopsy ; Keloid/pathology ; Keloid/metabolism |
| مستخلص: | Acne keloidalis is a primary scarring alopecia characterized by longstanding inflammation in the scalp causing keloid-like scar formation and hair loss. Histologically, acne keloidalis is characterized by mixed leukocytic infiltrates in the acute stage followed by a granulomatous reaction and extensive fibrosis in the later stages. To further explore its pathogenesis, bulk RNA sequencing, single-cell RNA sequencing, and spatial transcriptomics were applied to occipital scalp biopsy specimens of lesional and adjacent no-lesional skin in patients with clinically active disease. Unbiased clustering revealed 19 distinct cell populations, including 2 notable populations: POSTN + fibroblasts with enriched extracellular matrix signatures and SPP1 + myeloid cells with an M2 macrophage phenotype. Cell communication analyses indicated that fibroblasts and myeloid cells communicated by SPP1 signaling networks in lesional skin. A reverse transcriptomics in silico approach identified corticosteroids as possessing the capability to reverse the gene expression signatures of SPP1 + myeloid cells and POSTN + fibroblasts. Intralesional corticosteroid injection greatly reduced SPP1 and POSTN gene expression as well as acne keloidalis disease activity. Spatial transcriptomics and immunofluorescence staining verified microanatomic specificity of SPP1 + myeloid cells and POSTN + fibroblasts with disease activity. In summary, the communication between POSTN + fibroblasts and SPP1 + myeloid cells by SPP1 axis may contribute to the pathogenesis of acne keloidalis. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acne keloidalis; POSTN(+) fibroblasts; SPP1(+) myeloid cells; Single-cell RNA sequencing; Spatial transcriptomics |
| المشرفين على المادة: | 106441-73-0 (Osteopontin) 0 (Cell Adhesion Molecules) |
| تواريخ الأحداث: | Date Created: 20240113 Date Completed: 20240622 Latest Revision: 20240711 |
| رمز التحديث: | 20240711 |
| DOI: | 10.1016/j.jid.2023.12.014 |
| PMID: | 38218364 |
| قاعدة البيانات: | MEDLINE |
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