Adipose biopsy techniques for studies in human exercise physiology.

التفاصيل البيبلوغرافية
العنوان:	Adipose biopsy techniques for studies in human exercise physiology.
المؤلفون:	Lynch CE; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Brandt AR; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Vincenty CS; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Robbins E; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Skiles C; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Minchev K; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Chambers TL; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Belangee A; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Trappe TA; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Trappe SW; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States.
المصدر:	American journal of physiology. Regulatory, integrative and comparative physiology [Am J Physiol Regul Integr Comp Physiol] 2024 Mar 01; Vol. 326 (3), pp. R220-R229. Date of Electronic Publication: 2024 Jan 15.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901230 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1490 (Electronic) Linking ISSN: 03636119 NLM ISO Abbreviation: Am J Physiol Regul Integr Comp Physiol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Bethesda, Md. : American Physiological Society
مواضيع طبية MeSH:	Obesity/metabolism , Adipokines, Male ; Humans ; Female ; Biopsy ; Cytokines ; RNA, Messenger/genetics ; Anti-Inflammatory Agents
مستخلص:	Adipose biopsy techniques are relatively undefined for exercise physiology research in individuals at or near normal weight. The purpose of this study was to compare the influence of two adipose biopsy techniques on tissue quality through measurements of adipocyte cell size, as well as mRNA and protein levels of select pro- and anti-inflammatory cytokines and adipokines. Thirteen participants (9 M, 4 W; 28 ± 4 yr; 27 ± 3 kg·m ^-2 ; V̇o 2max : 3.3 ± 0.7 L·min ^-1 ) underwent subcutaneous adipose biopsies on either side of the umbilicus (incision: ∼8 cm lateral, sampling area: ∼5 cm lateral) using 1 ) a 6-mm Bergström biopsy needle and 2 ) a mini-liposuction approach with a 4-mm Mercedes biopsy needle that used prebiopsy tumescent delivery (∼30 mL 0.9% NaCl solution) into the sampling area (i.e., 'wet' technique). Tissue obtained was processed identically for analysis and both techniques returned high-quality tissue for histology (similar % intact adipocytes), mRNA (RNA integrity numbers >7.0), and protein. Adipocyte size was similar ( P > 0.05) between both techniques (Bergström: 6,116 ± 1,652 μm ² , 554-23,522 µm ² ; Mercedes: 6,517 ± 952 μm ² , 926-21,969 µm ² ). There were also no differences ( P > 0.05) between the two techniques for the measured cytokines (pro- and anti-inflammatory) and adipokines at the mRNA and protein levels. Adipocyte size was positively correlated with body mass index and body fat percentage, and negatively correlated with V̇o 2max ( P < 0.05). These results suggest both adipose biopsy techniques used in the current investigation are appropriate for histological, transcriptional, and translational level measurements in exercise physiology studies of nonobese women and men. NEW & NOTEWORTHY This study provides investigators with useful information related to adipose biopsy sampling approaches that can be used when planning studies that use measurements of adipose histology, as well as measurements at the mRNA and protein level. Adipose periumbilical sampling with the Bergström biopsy needle and the Mercedes wet mini-liposuction technique are both appropriate options for studies in exercise physiology and in nonobese individuals.
معلومات مُعتمدة:	The Human Bioenergetics Program, Ball State University
فهرسة مساهمة:	Keywords: Bergström; Mercedes; adipose; biopsy; histology
المشرفين على المادة:	0 (Adipokines) 0 (Cytokines) 0 (RNA, Messenger) 0 (Anti-Inflammatory Agents)
تواريخ الأحداث:	Date Created: 20240115 Date Completed: 20240226 Latest Revision: 20240226
رمز التحديث:	20240226
DOI:	10.1152/ajpregu.00266.2023
PMID:	38223939
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1522-1490
DOI:	10.1152/ajpregu.00266.2023