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Comparative assessment of emulsifiers for in vitro ruminal gas production and fermentation measurements: Tween 80 is a suitable emulsifier.

التفاصيل البيبلوغرافية
العنوان: Comparative assessment of emulsifiers for in vitro ruminal gas production and fermentation measurements: Tween 80 is a suitable emulsifier.
المؤلفون: Sun X; Department of Environmental Systems Science, ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland., Li Y; Department of Environmental Systems Science, ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland., Giller K; Department of Environmental Systems Science, ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland., Kunz C; Department of Environmental Systems Science, ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland., Terranova M; AgroVet-Strickhof, ETH Zürich, Lindau, Switzerland., Niu M; Department of Environmental Systems Science, ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland.
المصدر: Journal of animal physiology and animal nutrition [J Anim Physiol Anim Nutr (Berl)] 2024 May; Vol. 108 (3), pp. 680-690. Date of Electronic Publication: 2024 Jan 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Science Country of Publication: Germany NLM ID: 101126979 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0396 (Electronic) Linking ISSN: 09312439 NLM ISO Abbreviation: J Anim Physiol Anim Nutr (Berl) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Berlin] : Blackwell Science, c2001-
مواضيع طبية MeSH: Rumen*/metabolism , Fermentation* , Polysorbates*/pharmacology , Polysorbates*/chemistry , Emulsifying Agents*/chemistry , Emulsifying Agents*/pharmacology, Animals ; Cattle ; Female ; Animal Feed/analysis
مستخلص: Emulsifiers are essential for achieving a homogenous distribution of lipophilic supplements in in vitro rumen fluid incubations. Since emulsifiers can alter rumen fermentation, it is crucial to select one that minimally impacts fermentation parameters to reduce potential biases. This study aimed to evaluate seven emulsifiers' impact on in vitro ruminal fermentation using the Hohenheim Gas Test in order to identify the most inert emulsifier. Rumen fluids were collected from three non-lactating Original Brown-Swiss cannulated cows before morning feeding and incubated for 24 h with a basal diet in triplicates. The emulsifiers tested were ethanol, ethyl acetate, propylene glycol, glycerol, ethylene glycol, soy lecithin, and Tween® 80, each in two dosages (0.5% or 1% v/v). The untreated basal diet served as control. Compared to control, in vitro organic matter digestibility was enhanced by ethyl acetate (by 36.9 and 48.2%), ethylene glycol (by 20.6 and 20.1%), glycerol (by 46.9 and 56.8%) and soy lecithin (by 19.7 and 26.8%) at 0.5 and 1% dosage, respectively. Additionally, the 24-h methane production increased for ethanol (by 41.9 and 46.2%), ethylene glycol (by 50.5 and 51.5%), and glycerol (by 63.1 and 65.4%) for the 0.5 and 1% dosage, respectively, and 0.5% dosage for ethyl acetate (by 31.6%). The acetate molar proportion was 17.2%pt higher for ethyl acetate, and 25.5%pt lower for glycerol at 1% dosage, compared to the control. The propionate concentration was 22.1%pt higher 1% glycerol, and 15.2%pt and 15.1%pt higher for 0.5 and 1% propylene glycol, respectively, compared to the control. In summary, Tween® 80 did not significantly affect in vitro rumen fermentation parameters, making it the most suitable choice for in vitro incubations involving lipophilic substances in rumen fluid. Ethanol may be considered as an alternative emulsifier if methane production is not the variable of interest.
(© 2024 The Authors. Journal of Animal Physiology and Animal Nutrition published by Wiley‐VCH GmbH.)
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معلومات مُعتمدة: The study was funded by ETH Zurich.
فهرسة مساهمة: Keywords: gas production; lipophilic substances; organic solvent; rumen fermentation
المشرفين على المادة: 0 (Polysorbates)
0 (Emulsifying Agents)
تواريخ الأحداث: Date Created: 20240115 Date Completed: 20240507 Latest Revision: 20240507
رمز التحديث: 20240507
DOI: 10.1111/jpn.13924
PMID: 38223976
قاعدة البيانات: MEDLINE
الوصف
تدمد:1439-0396
DOI:10.1111/jpn.13924