دورية أكاديمية
The immunosuppressive, growth-hindering, hepatotoxic, and oxidative stress and immune related-gene expressions-altering effects of gibberellic acid in Oreochromis niloticus: A mitigation trial using alpha-lipoic acid.
| العنوان: | The immunosuppressive, growth-hindering, hepatotoxic, and oxidative stress and immune related-gene expressions-altering effects of gibberellic acid in Oreochromis niloticus: A mitigation trial using alpha-lipoic acid. |
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| المؤلفون: | El-Houseiny W; Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt. Electronic address: drwalaaelhouseiny@yahoo.com., Arisha AH; Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Badr City, Cairo, Egypt; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Behairy A; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Metwally MMM; Department of Pathology and Clinical pathology, Faculty of Veterinary Medicine, King Salman international University, Ras sidr, Egypt.; Department of pathology, Faculty of Veterinary Medicine, Zagazig university, Zagazig 44519, Egypt., Abdel-Warith AA; Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Younis EM; Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Davies SJ; Aquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, H91V8Y1 Galway, Ireland., Hassan BA; Pharmacology Department, Faculty of Pharmacy, Future University, Cairo 11835, Egypt., Abd-Elhakim YM; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt. Electronic address: yasmina_forensic@hotmail.com. |
| المصدر: | Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Jan; Vol. 198, pp. 105725. Date of Electronic Publication: 2023 Dec 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York Ny : Academic Press |
| مواضيع طبية MeSH: | Thioctic Acid*/pharmacology , Thioctic Acid*/metabolism , Cichlids*/genetics , Gibberellins*, Animals ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Dietary Supplements ; Oxidative Stress ; Gene Expression |
| مستخلص: | This study aimed to examine the effects of gibberellic acid (GBA) on growth, hemato-biochemical parameters related to liver functions, digestive enzymes, and immunological response in Oreochromis niloticus. Besides, the probable underlying mechanisms were explored by assessing antioxidant, apoptotic, and immune-related gene expression. Furthermore, the likelihood of restoration following alpha-lipoic acid (LIP) dietary supplementation was explored. The fish (average initial weight 30.75 ± 0.46) were equally classified into four groups: the control group, the LIP group (fed on a basal diet plus 600 mg/kg of LIP), the GBA group (exposed to 150 mg GBA/L), and the GBA + LIP group (exposed to 150 mg GBA/L and fed a diet containing LIP and GBA) for 60 days. The study findings showed that LIP supplementation significantly reduced GBA's harmful effects on survival rate, growth, feed intake, digestive enzymes, and antioxidant balance. Moreover, the GBA exposure significantly increased liver enzymes, stress markers, cholesterol, and triglyceride levels, all of which were effectively mitigated by the supplementation of LIP. Additionally, LIP addition to fish diets significantly minimized the histopathological alterations in the livers of GBA-treated fish, including fatty change, sharply clear cytoplasm with nuclear displacement to the cell periphery, single-cell necrosis, vascular congestion, and intralobular hemorrhages. The GBA-induced reduction in lysozyme activity, complement C3, and nitric oxide levels, together with the downregulation of antioxidant genes (cat and sod), was significantly restored by dietary LIP. Meanwhile, adding LIP to the GBA-exposed fish diets significantly corrected the aberrant expression of hsp70, caspase- 3, P53, pcna, tnf-a, and il-1β in O. niloticus liver. Conclusively, dietary LIP supplementation could mitigate the harmful effects of GBA exposure on fish growth and performance, physiological conditions, innate immunity, antioxidant capability, inflammatory response, and cell apoptosis. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Heat shock protein 70; Immunosuppression; Lipoic acid; Plant growth regulators; mRNA profiling |
| المشرفين على المادة: | 0 (Antioxidants) 73Y7P0K73Y (Thioctic Acid) BU0A7MWB6L (gibberellic acid) 0 (Gibberellins) |
| تواريخ الأحداث: | Date Created: 20240115 Date Completed: 20240117 Latest Revision: 20240117 |
| رمز التحديث: | 20240117 |
| DOI: | 10.1016/j.pestbp.2023.105725 |
| PMID: | 38225080 |
| قاعدة البيانات: | MEDLINE |
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