دورية أكاديمية
Mixta mediterraneensis as a novel and abundant gut symbiont of the allergen-producing domestic mite Blomia tropicalis.
العنوان: | Mixta mediterraneensis as a novel and abundant gut symbiont of the allergen-producing domestic mite Blomia tropicalis. |
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المؤلفون: | Erban T; Crop Research Institute, Drnovska 507/73, 161 06, Prague 6 - Ruzyne, Czechia., Sopko B; Crop Research Institute, Drnovska 507/73, 161 06, Prague 6 - Ruzyne, Czechia., Klimov PB; Purdue University, Lilly Hall of Life Sciences, G-225, 915 W State St, West Lafayette, IN, 47907, USA., Hubert J; Crop Research Institute, Drnovska 507/73, 161 06, Prague 6 - Ruzyne, Czechia. hubert@vurv.cz.; Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 00, Prague 6 - Suchdol, Czechia. hubert@vurv.cz. |
المصدر: | Experimental & applied acarology [Exp Appl Acarol] 2024 Feb; Vol. 92 (2), pp. 161-181. Date of Electronic Publication: 2024 Jan 16. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8507436 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1572-9702 (Electronic) Linking ISSN: 01688162 NLM ISO Abbreviation: Exp Appl Acarol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1999- : Dordrecht : Kluwer Academic Publishers Original Publication: Amsterdam ; New York : Elsevier, [c1985- |
مواضيع طبية MeSH: | Allergens* , Mites*, Humans ; Animals |
مستخلص: | Blomia tropicalis is an allergen-producing mite in the human environment in tropical regions. The microbiome of B. tropicalis was described using the barcode sequencing region of V4 16S rDNA and genome assemblage. Mixta mediterraneensis, previously isolated from human skin swabs, was identified as a B. tropicalis gut symbiont based on genome assembly. The microbiome contains two bacteria, Staphylococcus and M. mediterraneensis. The number of M. mediterraneensis 16S DNA copies was 10 6 per mite and 10 9 per feces in the rearing chamber based on qPCR quantification. The profile of this bacterium reached 50% of reads in the mite gut and feces. Genomic analyses revealed that the bacterium has several metabolic pathways that suggest metabolic cooperation with the mite host in vitamin and amino acid synthesis, nitrogen recycling, and antimicrobial defense. Lysozyme is present in the symbiotic bacterium but absent in the mite. The B. tropicalis microbiome contained Staphylococcus, which accelerates mite population growth. Mites can digest Staphylococcus by using specific enzymes with hydrolytic functions against bacterial cell walls (chitinases and cathepsin D), leading to endocytosis of bacteria and their degradation in lysosomes and phagosomes. Gene expression analysis of B. tropicalis indicated that phagocytosis was mediated by the PI3-kinase/Akt pathway interacting with the invasins produced by M. mediterraneensis. Moreover, the symbiont had metabolic pathways that allowed it to recycle the mite metabolic waste product guanine, known as a mite attractant. The mite host symbiont enhances mite aggregation in the feces, and the fecal-oral transmission route is excepted. (© 2024. The Author(s).) |
References: | Appl Environ Microbiol. 2009 Dec;75(23):7537-41. (PMID: 19801464) Mol Biol Evol. 2022 May 3;39(5):. (PMID: 35535514) Cell Rep. 2021 Dec 28;37(13):110178. (PMID: 34965421) Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) Proc Natl Acad Sci U S A. 2012 May 15;109(20):E1230-7. (PMID: 22517738) PLoS One. 2014 Nov 19;9(11):e112963. (PMID: 25409509) Sci Rep. 2016 Jul 08;6:29505. (PMID: 27389097) Curr Protoc. 2021 Dec;1(12):e323. (PMID: 34936221) Appl Environ Microbiol. 2013 Sep;79(17):5112-20. (PMID: 23793624) Nat Commun. 2019 May 16;10(1):2182. (PMID: 31097708) Future Microbiol. 2012 Feb;7(2):281-9. (PMID: 22324995) Bioinformatics. 2016 Apr 1;32(7):1009-15. (PMID: 26589280) Bioinformatics. 2007 Nov 1;23(21):2947-8. (PMID: 17846036) J Appl Microbiol. 2015 Feb;118(2):470-84. (PMID: 25469657) Science. 2009 Nov 20;326(5956):1120-3. (PMID: 19965433) Genome Biol. 2009;10(3):R25. (PMID: 19261174) Environ Entomol. 2009 Feb;38(1):116-20. (PMID: 19791604) Nat Commun. 2018 Nov 30;9(1):5114. (PMID: 30504855) Nat Biotechnol. 2011 May 15;29(7):644-52. (PMID: 21572440) FEMS Microbiol Ecol. 2021 Mar 31;97(4):. (PMID: 33674831) Front Physiol. 2016 Feb 24;7:53. (PMID: 26941650) Mol Biol Evol. 2015 Oct;32(10):2798-800. (PMID: 26130081) Nucleic Acids Res. 2006 Jan 1;34(Database issue):D354-7. (PMID: 16381885) Acarologia. 1974 Mar;15(3):477-89. (PMID: 4446908) ISME J. 2012 Aug;6(8):1621-4. (PMID: 22402401) Exp Appl Acarol. 2015 Jan;65(1):73-87. (PMID: 25100296) Nucleic Acids Res. 2017 Jan 4;45(D1):D37-D42. (PMID: 27899564) J Mol Biol. 2000 Sep 8;302(1):205-17. (PMID: 10964570) J Proteomics. 2021 Oct 30;249:104356. (PMID: 34438106) Nucleic Acids Res. 2011 Jul;39(Web Server issue):W29-37. (PMID: 21593126) Nat Methods. 2013 Oct;10(10):996-8. (PMID: 23955772) Biosci Microbiota Food Health. 2016;35(4):173-184. (PMID: 27867804) Nat Biotechnol. 2022 Jul;40(7):1023-1025. (PMID: 34980915) Sci Rep. 2017 Nov 15;7(1):15610. (PMID: 29142289) J Allergy Clin Immunol. 2005 Dec;116(6):1296-300. (PMID: 16337462) Proc Natl Acad Sci U S A. 2015 Dec 22;112(51):15678-83. (PMID: 26644557) Genome Biol Evol. 2015 Jan 12;7(2):620-35. (PMID: 25587021) Mol Biol Evol. 2021 Sep 27;38(10):4647-4654. (PMID: 34320186) Microb Ecol. 2019 May;77(4):1048-1066. (PMID: 30465068) Nat Biotechnol. 2008 Dec;26(12):1367-72. (PMID: 19029910) J Proteome Res. 2011 Apr 1;10(4):1794-805. (PMID: 21254760) J Morphol. 2008 Jan;269(1):54-71. (PMID: 17886888) Mol Genet Genomics. 2022 Jan;297(1):213-225. (PMID: 34988605) BMC Bioinformatics. 2013 Feb 21;14:60. (PMID: 23432962) Exp Appl Acarol. 2008 Mar;44(3):199-212. (PMID: 18357505) PLoS One. 2014 Nov 11;9(11):e112919. (PMID: 25387104) PLoS One. 2013 Jun 21;8(6):e67115. (PMID: 23805294) Nucleic Acids Res. 2022 Jan 7;50(D1):D801-D807. (PMID: 34634793) Bioinformatics. 2017 Sep 15;33(18):2946-2947. (PMID: 28525531) FEMS Microbiol Ecol. 2021 Sep 16;97(10):. (PMID: 34448854) Microb Ecol. 2012 May;63(4):919-28. (PMID: 22057398) Front Microbiol. 2016 Jul 12;7:1046. (PMID: 27462300) Behav Res Methods. 2020 Apr;52(2):464-488. (PMID: 31152384) J Proteomics. 2017 Jun 6;162:11-19. (PMID: 28442447) New Microbes New Infect. 2021 Jan 15;40:100840. (PMID: 33604053) J Comput Biol. 2012 May;19(5):455-77. (PMID: 22506599) Environ Microbiol. 2021 Jan;23(1):36-50. (PMID: 32686279) Nucleic Acids Res. 2014 Jan;42(Database issue):D633-42. (PMID: 24288368) Bioinformatics. 2018 Mar 15;34(6):1037-1039. (PMID: 29106469) Sci Rep. 2016 Sep 09;6:33168. (PMID: 27609055) J Mol Biol. 2016 Feb 22;428(4):726-731. (PMID: 26585406) Mol Cell Proteomics. 2014 Sep;13(9):2513-26. (PMID: 24942700) Proc Int Conf Intell Syst Mol Biol. 1995;3:114-20. (PMID: 7584426) Clin Transl Allergy. 2023 Oct;13(10):e12302. (PMID: 37876035) Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173) PLoS One. 2015 Jul 08;10(7):e0129996. (PMID: 26154283) Nat Methods. 2016 Sep;13(9):731-40. (PMID: 27348712) Exp Appl Acarol. 2020 Mar;80(3):363-380. (PMID: 32072355) Bioinformatics. 2018 Sep 15;34(18):3094-3100. (PMID: 29750242) J Econ Entomol. 2016 Aug;109(4):1887-96. (PMID: 27122496) Genome Biol. 2016 Jun 20;17(1):132. (PMID: 27323842) FEMS Microbiol Rev. 2015 Nov;39(6):968-84. (PMID: 26109597) J Proteomics. 2020 Jan 6;210:103535. (PMID: 31629957) Nat Microbiol. 2016 Jan 11;1:15011. (PMID: 27571756) J Clin Microbiol. 2002 Apr;40(4):1333-8. (PMID: 11923353) Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063) BMC Bioinformatics. 2009 Dec 15;10:421. (PMID: 20003500) J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712) Microbiology (Reading). 2000 Dec;146 Pt 12:3061-3069. (PMID: 11101664) Nucleic Acids Res. 2007;35(9):3100-8. (PMID: 17452365) FEMS Microbiol Ecol. 2017 Jan;93(1):. (PMID: 27794015) Stand Genomic Sci. 2014 Dec 08;9:2. (PMID: 25780495) |
معلومات مُعتمدة: | MZE-RO0423 Ministerstvo Zemědělství; LUAUS23082 Ministerstvo Školství, Mládeže a Tělovýchovy; RF 193021X0012 Ministry of Science and Higher Education of the Russian Federation |
فهرسة مساهمة: | Keywords: Allergen; Digestion; Enzyme; Mite; Symbionts |
المشرفين على المادة: | 0 (Allergens) |
تواريخ الأحداث: | Date Created: 20240116 Date Completed: 20240312 Latest Revision: 20240314 |
رمز التحديث: | 20240314 |
مُعرف محوري في PubMed: | PMC10927894 |
DOI: | 10.1007/s10493-023-00875-3 |
PMID: | 38227156 |
قاعدة البيانات: | MEDLINE |
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