دورية أكاديمية
أعرض في EDS

Poly-γ-glutamic acid overproduction of Bacillus licheniformis ATCC 9945 a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches.

التفاصيل البيبلوغرافية
العنوان: Poly-γ-glutamic acid overproduction of Bacillus licheniformis ATCC 9945 a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches.
المؤلفون: Ebrahimzadeh Kouchesfahani M; Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran., Bahrami A; Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran., Babaeipour V; Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran.
المصدر: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2024 Jun; Vol. 71 (3), pp. 565-583. Date of Electronic Publication: 2024 Jan 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8609465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1470-8744 (Electronic) Linking ISSN: 08854513 NLM ISO Abbreviation: Biotechnol Appl Biochem Subsets: MEDLINE
أسماء مطبوعة: Publication: Jan. 2011- : Malden : Wiley-Blackwell
Original Publication: San Diego : Academic Press, [cl986]-
مواضيع طبية MeSH: Polyglutamic Acid*/biosynthesis , Polyglutamic Acid*/analogs & derivatives , Polyglutamic Acid*/metabolism , Polyglutamic Acid*/chemistry , Bacillus licheniformis*/metabolism , Bacillus licheniformis*/growth & development , Culture Media*/chemistry , Culture Media*/metabolism , Glutamic Acid*/metabolism, Fermentation ; Research Design
مستخلص: The commercial production of multifunctional, biocompatible, and biodegradable biopolymers such as poly-γ-glutamic acid via microbial fermentation requires the development of simple and cheap methods for mass production. This study optimized the poly-γ-glutamic acid production of Bacillus licheniformis ATCC 9945 a in several steps. At first, the most critical components of the culture medium, including l-glutamic acid, citric acid, and glycerol, were selected by screening nine factors through the Plackett-Burman experimental design and then were optimized using the response surface method and the central composite design algorithm. Under optimal conditions, the production of poly-γ-glutamic acid increased by more than 4.2 times from 11.2 to 47.2 g/L. This is one of the highest production rates of this strain in submerged batch fermentation reported so far using the optimized medium compared to the conventional base medium. A novel and efficient sudden pulse feeding strategy (achieved by a novel one-factorial statistical technique) of l-glutamic acid to the optimized medium increased biopolymer production from 47.2 to 66.1 g/L, the highest value reported in published literature with this strain. This simple, reproducible, and cheap fermentation process can considerably enhance the commercial applications of the poly-γ-glutamic acid synthesized by B. licheniformis ATCC 9945 a .
(© 2024 International Union of Biochemistry and Molecular Biology, Inc.)
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فهرسة مساهمة: Keywords: Bacillus licheniformis; poly‐γ‐glutamic acid; response surface optimization
المشرفين على المادة: 25513-46-6 (Polyglutamic Acid)
0 (Culture Media)
0 (poly(gamma-glutamic acid))
3KX376GY7L (Glutamic Acid)
تواريخ الأحداث: Date Created: 20240121 Date Completed: 20240610 Latest Revision: 20240610
رمز التحديث: 20240610
DOI: 10.1002/bab.2559
PMID: 38246886
قاعدة البيانات: MEDLINE
الوصف
تدمد:1470-8744
DOI:10.1002/bab.2559