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Eugenol alleviates acrylamide-induced rat testicular toxicity by modulating AMPK/p-AKT/mTOR signaling pathway and blood-testis barrier remodeling.

التفاصيل البيبلوغرافية
العنوان: Eugenol alleviates acrylamide-induced rat testicular toxicity by modulating AMPK/p-AKT/mTOR signaling pathway and blood-testis barrier remodeling.
المؤلفون: Saleh DO; Pharmacology Department, National Research Centre, Giza, 12622, Egypt. doabdelfattah@yahoo.com., Baraka SM; Chemistry of Natural Compounds Department, National Research Centre, Giza, 12622, Egypt., Jaleel GAA; Pharmacology Department, National Research Centre, Giza, 12622, Egypt., Hassan A; Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Ahmed-Farid OA; Physiology Department, National Organization for Drug Control and Research, Giza, Egypt.
المصدر: Scientific reports [Sci Rep] 2024 Jan 22; Vol. 14 (1), pp. 1910. Date of Electronic Publication: 2024 Jan 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Testis* , Antifibrinolytic Agents*, Male ; Animals ; Rats ; AMP-Activated Protein Kinases ; Eugenol/pharmacology ; Proto-Oncogene Proteins c-akt ; Blood-Testis Barrier ; Phosphatidylinositol 3-Kinases ; Semen ; Signal Transduction ; TOR Serine-Threonine Kinases ; Acrylamide/toxicity ; Amino Acids ; Mammals
مستخلص: This study aimed to investigate the effects of eugenol treatment on reproductive parameters in acrylamide (ACR)-intoxicated rats. The study evaluated alterations in relative testes and epididymides weights, sperm quality, serum hormonal status, seminal plasma amino acids, testicular cell energy and phospholipids content, oxidative and nitrosative stress parameters, adenosine monophosphate-activated protein kinase/ phosphoinositide 3-kinase/phosphor-protein kinase B/mammalian target of rapamycin (AMPK/PI3K/p-AKT/mTOR) signaling pathway, blood-testis barrier (BTB) remodeling markers, testicular autophagy and apoptotic markers, as well as histopathological alterations in testicular tissues. The results revealed that eugenol treatment demonstrated a significant improvement in sperm quality parameters, with increased sperm cell concentration, progressive motility live sperm, and a reduction in abnormal sperm, compared to the ACR-intoxicated group. Furthermore, eugenol administration increased the levels of seminal plasma amino acids in a dose-dependent manner. In addition, eugenol treatment dose-dependently improved testicular oxidative/nitrosative stress biomarkers by increasing oxidized and reduced glutathione levels and reducing malondialdehyde and nitric oxide contents as compared to ACRgroup. However, eugenol treatment at a high dose restored the expression of AMPK, PI3K, and mTOR genes, to levels comparable to the control group, while significantly increasing p-AKT content compared to the ACRgroup. In conclusion, the obtained findings suggest the potential of eugenol as a therapeutic agent in mitigating ACR-induced detrimental effects on the male reproductive system via amelioration of ROS-mediated autophagy, apoptosis, AMPK/p-AKT/mTOR signaling pathways and BTB remodeling.
(© 2024. The Author(s).)
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المشرفين على المادة: EC 2.7.11.31 (AMP-Activated Protein Kinases)
3T8H1794QW (Eugenol)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
EC 2.7.11.1 (TOR Serine-Threonine Kinases)
20R035KLCI (Acrylamide)
0 (Amino Acids)
0 (Antifibrinolytic Agents)
EC 2.7.1.1 (mTOR protein, rat)
تواريخ الأحداث: Date Created: 20240122 Date Completed: 20240124 Latest Revision: 20240126
رمز التحديث: 20240127
مُعرف محوري في PubMed: PMC10803763
DOI: 10.1038/s41598-024-52259-1
PMID: 38253778
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-52259-1