دورية أكاديمية
Chip-Based Molecular Evaluation of a DNA Extraction Protocol for Candida Species from Positive Blood Cultures.
| العنوان: | Chip-Based Molecular Evaluation of a DNA Extraction Protocol for Candida Species from Positive Blood Cultures. |
|---|---|
| المؤلفون: | Ivagnes V; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Menchinelli G; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Liotti FM; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., De Carolis E; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Torelli R; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., De Lorenzis D; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Recine C; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Sanguinetti M; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., D'Inzeo T; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy., Posteraro B; Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Dipartimento di Scienze Mediche e Chirurgiche Addominali ed Endocrino Metaboliche, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. |
| المصدر: | Microorganisms [Microorganisms] 2023 Dec 31; Vol. 12 (1). Date of Electronic Publication: 2023 Dec 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2013]- |
| مستخلص: | The diagnosis of Candida bloodstream infection (BSI) may rely on a PCR-based analysis of a positive blood culture (PBC) obtained from the patient at the time of BSI. In this study, a yeast DNA extraction protocol for use on PBCs was developed and evaluated with the molecular mouse (MM) yeast blood (YBL) chip-based PCR assay, which allowed us to detect nine medically relevant Candida species. We studied 125 simulated or clinical PBCs for Candida species. A positive correlation between the DNA concentration and colony-forming unit count was found for simulated (Spearman's ρ = 0.58; p < 0.0001) and clinical (Spearman's ρ = 0.23, p = 0.09) PBCs. The extracted DNA yielded positive results with the MM YBL chip assay that agreed with the Candida species-level identification results for 63 (100%) of 63 isolates from simulated PBCs and 66 (99.5%) of 67 isolates from clinical PBCs. The false-negative result was for one C. tropicalis isolate that grew together with C. albicans in PBC. None of the 30 ( Candida )-negative clinical BCs included as negative controls yielded a positive result with the MM YBL chip assay. Our DNA extraction protocol for the Candida species couples efficiency and simplicity together. Nevertheless, further studies are needed before it can be adopted for use with the MM YBL chip assay. |
| References: | Expert Rev Mol Diagn. 2023 Jun;23(6):475-484. (PMID: 37198873) Int Microbiol. 2018 Sep;21(3):107-119. (PMID: 30810955) Curr Protoc. 2023 Apr;3(4):e749. (PMID: 37097192) J Fungi (Basel). 2021 Mar 19;7(3):. (PMID: 33808911) Nat Rev Dis Primers. 2018 May 11;4:18026. (PMID: 29749387) Infect Dis Clin North Am. 2021 Jun;35(2):389-413. (PMID: 34016283) Curr Drug Targets. 2020;21(4):365-373. (PMID: 31549952) Front Microbiol. 2019 Feb 19;10:221. (PMID: 30837964) J Fungi (Basel). 2020 Feb 03;6(1):. (PMID: 32028622) Clin Microbiol Infect. 2022 Feb;28(2):195-201. (PMID: 34687856) Expert Rev Anti Infect Ther. 2014 Mar;12(3):375-82. (PMID: 24506174) Microorganisms. 2021 Jun 12;9(6):. (PMID: 34204665) PLoS One. 2020 Mar 23;15(3):e0226467. (PMID: 32203515) J Clin Microbiol. 2016 Mar;54(3):576-84. (PMID: 26677254) Vet World. 2021 Jun;14(6):1608-1614. (PMID: 34316210) Sensors (Basel). 2018 Aug 07;18(8):. (PMID: 30087266) J Clin Microbiol. 2020 Mar 25;58(4):. (PMID: 31996441) Clin Microbiol Rev. 2017 Nov 15;31(1):. (PMID: 29142077) Microbiol Spectr. 2023 Jan 30;:e0410422. (PMID: 36715537) Animals (Basel). 2022 Feb 16;12(4):. (PMID: 35203197) Microorganisms. 2023 Mar 23;11(4):. (PMID: 37110241) |
| معلومات مُعتمدة: | PE00000007, INF-ACT European Commission |
| فهرسة مساهمة: | Keywords: Candida species; PCR assay; blood culture; bloodstream infection; molecular detection; yeast blood chip |
| تواريخ الأحداث: | Date Created: 20240123 Latest Revision: 20240129 |
| رمز التحديث: | 20240129 |
| مُعرف محوري في PubMed: | PMC10821462 |
| DOI: | 10.3390/microorganisms12010081 |
| PMID: | 38257908 |
| قاعدة البيانات: | MEDLINE |
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