دورية أكاديمية
Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface-Supported A-PoD.
| العنوان: | Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface-Supported A-PoD. |
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| المؤلفون: | Jang H; Department of Bioengineering, University of California San Diego, La Jolla, California., Li Y; Department of Bioengineering, University of California San Diego, La Jolla, California., Wu S; Department of Bioengineering, University of California San Diego, La Jolla, California., Shi L; Department of Bioengineering, University of California San Diego, La Jolla, California. |
| المصدر: | Current protocols [Curr Protoc] 2024 Jan; Vol. 4 (1), pp. e970. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101773894 Publication Model: Print Cited Medium: Internet ISSN: 2691-1299 (Electronic) Linking ISSN: 26911299 NLM ISO Abbreviation: Curr Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hoboken, NJ : John Wiley & Sons, [2021]- |
| مواضيع طبية MeSH: | Nonlinear Optical Microscopy* , Microscopy*, Algorithms ; Deuterium Oxide ; Product Labeling |
| مستخلص: | Raman microscopy is a vibrational imaging technology that can detect molecular chemical bond vibrational signals. Since this signal is originated from almost every vibrational mode of molecules with different vibrational energy levels, it provides spatiotemporal distribution of various molecules in living organisms without the need for any labeling. The limitations of low signal strength in Raman microscopy have been effectively addressed by incorporating a stimulated emission process, leading to the development of stimulated Raman scattering (SRS) microscopy. Furthermore, the issue of low spatial resolution has been resolved through the application of computational techniques, specifically image deconvolution. In this article, we present a comprehensive guide to super-resolution SRS microscopy using an Adam-based pointillism deconvolution (A-PoD) algorithm, complemented by a user-friendly graphical user interface (GUI). We delve into the crucial parameters and conditions necessary for achieving super-resolved images through SRS imaging. Additionally, we provide a step-by-step walkthrough of the preprocessing steps and the use of GUI-supported A-PoD. This complete package offers a user-friendly platform for super-resolution SRS microscopy, enhancing the versatility and applicability of this advanced microscopy technique to reveal nanoscopic multimolecular nature. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Super-resolution stimulated Raman scattering microscopy with graphical user interface-supported A-PoD Support Protocol: Deuterium labeling on cells with heavy water for metabolic imaging. (© 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.) |
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| معلومات مُعتمدة: | U54 HL165443 United States HL NHLBI NIH HHS; R21 NS125395 United States NS NINDS NIH HHS; U54 CA132378 United States CA NCI NIH HHS; R01 GM149976 United States GM NIGMS NIH HHS; U54 DK134301 United States DK NIDDK NIH HHS; R01 NS111039 United States NS NINDS NIH HHS |
| فهرسة مساهمة: | Keywords: deconvolution; metabolic imaging; stimulated Raman scattering; super-resolution |
| المشرفين على المادة: | J65BV539M3 (Deuterium Oxide) |
| تواريخ الأحداث: | Date Created: 20240125 Date Completed: 20240126 Latest Revision: 20240203 |
| رمز التحديث: | 20240205 |
| مُعرف محوري في PubMed: | PMC10832363 |
| DOI: | 10.1002/cpz1.970 |
| PMID: | 38270527 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2691-1299 |
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| DOI: | 10.1002/cpz1.970 |