دورية أكاديمية
Developing rational scale-down simulators for mimicking substrate heterogeneities based on cell lifelines in industrial-scale bioreactors.
| العنوان: | Developing rational scale-down simulators for mimicking substrate heterogeneities based on cell lifelines in industrial-scale bioreactors. |
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| المؤلفون: | Zhao J; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai, People's Republic of China., Muawiya MA; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai, People's Republic of China., Zhuang Y; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai, People's Republic of China., Wang G; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai, People's Republic of China. Electronic address: guanwang@ecust.edu.cn. |
| المصدر: | Bioresource technology [Bioresour Technol] 2024 Mar; Vol. 395, pp. 130354. Date of Electronic Publication: 2024 Jan 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| مواضيع طبية MeSH: | Bioreactors* , Penicillium chrysogenum*/metabolism |
| مستخلص: | The influence of extracellular variations on the cellular metabolism and thereby the process performance at large-scale can be evaluated using the so-called scale-down simulators. Nevertheless, the major challenge is to design an appropriate scale-down simulator, which can accurately mimic the cell lifelines that record the flow paths and experiences of cells circulating in large-scale bioreactors. To address this, a dedicated SDSA (scale-down simulator application) was purposedly developed on the basis of black box model and process reaction model established for Penicillium chrysogenum strain as well as cell lifelines or trajectories information in an industrial-scale fermentor. Guided by the SDSA, the industrial-relevant metabolic regimes for substrate availability, i.e., excess, limitation and starvation, were successfully reproduced at laboratory-scale three-compartment scale-down (SD) system. In addition, such SDSA can also display individual process dynamics in each compartment, and demonstrate how individual factors influence the entire bioprocess performance, thus serving both educational and research purposes. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell lifelines; Heterogeneity; Penicillium chrysogenum; Scale-down; Substrate gradients |
| تواريخ الأحداث: | Date Created: 20240125 Date Completed: 20240219 Latest Revision: 20240219 |
| رمز التحديث: | 20240219 |
| DOI: | 10.1016/j.biortech.2024.130354 |
| PMID: | 38272147 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2024.130354 |