دورية أكاديمية
أعرض في EDS

Topical Losartan Inhibition of Myofibroblast Generation in Rabbit Corneas With Acute Incisions.

التفاصيل البيبلوغرافية
العنوان: Topical Losartan Inhibition of Myofibroblast Generation in Rabbit Corneas With Acute Incisions.
المؤلفون: Martinez VV; The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH; and., Dutra BAL; The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH; and.; Department of Ophthalmology, University of Sao Paulo, Sao Paulo, Brazil., Sampaio LP; The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH; and.; Department of Ophthalmology, University of Sao Paulo, Sao Paulo, Brazil., Shiju TM; The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH; and., Santhiago MR; Department of Ophthalmology, University of Sao Paulo, Sao Paulo, Brazil., Wilson SE; The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH; and.
المصدر: Cornea [Cornea] 2024 Jan 26. Date of Electronic Publication: 2024 Jan 26.
Publication Model: Ahead of Print
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 8216186 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1536-4798 (Electronic) Linking ISSN: 02773740 NLM ISO Abbreviation: Cornea Subsets: MEDLINE
أسماء مطبوعة: Publication: Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: New York, N.Y. : Masson Pub. USA, c1982-
مستخلص: Purpose: The purpose of this study was to study whether deep central corneal incisions close during topical losartan treatment and the effect of topical losartan on myofibroblast generation after incisions in rabbit corneas.
Methods: Rabbits (12) had a 0.35-mm deep radial incision from the center of the cornea into the limbus in 1 eye that was approximated with a single 10-0 nylon suture 1 mm inside the limbus. The incision was treated with 50 μL of topical 0.8 mg/mL losartan or 50 μL of balanced salt solution vehicle 6 times per day for 1 month. Standardized slitlamp photographs of the central incisions were analyzed for opacity with ImageJ before euthanasia. Triplex IHC was performed on cryofixed corneas for myofibroblast marker alpha-smooth muscle actin, mesenchymal cell marker vimentin, and basement membrane marker laminin alpha-5. Stromal α-SMA-positive myofibroblasts surrounding the incisions were quantitated with ImageJ.
Results: Topical losartan compared with vehicle did not affect closure of the radial incisions or the opacity that developed surrounding the incisions at 1 month after injury. Topical losartan compared with vehicle did significantly decrease the average density of stromal myofibroblasts surrounding the incisions.
Conclusions: Topical losartan, a known inhibitor of transforming growth factor beta signaling, did not affect closure of deep corneal incisions. Losartan decreased myofibroblast generation surrounding nearly full-thickness radial corneal incisions compared with vehicle. The opacity at the incisions was not significantly affected by losartan-likely because corneal fibroblasts that develop in the stroma adjacent to the incisions were not changed by the losartan compared with the vehicle.
(Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
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معلومات مُعتمدة: P30 EY025585 United States EY NEI NIH HHS; EY025585 United States EY NEI NIH HHS
تواريخ الأحداث: Date Created: 20240126 Latest Revision: 20240727
رمز التحديث: 20240727
مُعرف محوري في PubMed: PMC11272906
DOI: 10.1097/ICO.0000000000003476
PMID: 38277165
قاعدة البيانات: MEDLINE
الوصف
تدمد:1536-4798
DOI:10.1097/ICO.0000000000003476