دورية أكاديمية
Production of β-mannanase, inulinase, and oligosaccharides from coffee wastes and extracts.
| العنوان: | Production of β-mannanase, inulinase, and oligosaccharides from coffee wastes and extracts. |
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| المؤلفون: | Basmak S; Department of Food Engineering, Akdeniz University, Antalya 07058, Turkey., Turhan I; Department of Food Engineering, Akdeniz University, Antalya 07058, Turkey. Electronic address: iturhan@akdeniz.edu.tr. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Mar; Vol. 261 (Pt 1), pp. 129798. Date of Electronic Publication: 2024 Jan 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | beta-Mannosidase*/metabolism , Oligosaccharides*/metabolism , Aspergillus* , Glycoside Hydrolases*, Aspergillus niger ; Fermentation |
| مستخلص: | This study aimed to produce enzymes (beta (β)-mannanase using a recombinant Aspergillus sojae AsT3 and inulinase using Aspergillus niger A42) and oligosaccharides (mannooligosaccharides (MOS), fructooligosaccharides (FOS)) using coffee waste, ground coffee, and coffee extract by solid-state fermentation (SSF). Plackett-Burman Design (PBD) was used to create a design for enzyme production with four different parameters (temperature, pH, solid-to-liquid ratio (SLR), and mix with coffee wastes and ground coffee). The highest β-mannanase and inulinase activities were 71.17 and 564.07 U/mg of protein respectively. Statistical analysis showed that the temperature was statistically significant for the production of both enzymes (P < 0.05). The produced enzymes were utilized in French Pressed coffee extracts to produce oligosaccharides. As a result of the enzymatic hydrolyzation, the highest mannobiose, mannotriose, mannotetraose, and total MOS levels were 109.66, 101.11, 391.02, and 600.64 ppm, respectively. For the FOS production, the maximal 1,1,1-kestopentaose was 38.34 ppm. Consequently, this study demonstrates that a recombinant Aspergillus sojae AsT3 β-mannanase and Aspergillus niger A42 inulinase produced from coffee wastes and ground coffee can be used in coffee extracts to increase the amount of oligosaccharides in coffee extracts. Competing Interests: Declaration of competing interest The authors have declared no competing interest in this article. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Fructooligosaccharides; Inulinase; Mannooligosaccharides; Plackett-Burman Design; Solid-state fermentation; β-Mannanase |
| المشرفين على المادة: | EC 3.2.1.25 (beta-Mannosidase) EC 3.2.1.7 (inulinase) 0 (Oligosaccharides) EC 3.2.1.- (Glycoside Hydrolases) |
| SCR Organism: | Aspergillus sojae; Aspergillus brasiliensis |
| تواريخ الأحداث: | Date Created: 20240129 Date Completed: 20240311 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| DOI: | 10.1016/j.ijbiomac.2024.129798 |
| PMID: | 38286365 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.129798 |