دورية أكاديمية
أعرض في EDS

Common Markers of Muscle Damage Are Associated with Divergent Gene Expression Patterns after Eccentric Contractions.

التفاصيل البيبلوغرافية
العنوان: Common Markers of Muscle Damage Are Associated with Divergent Gene Expression Patterns after Eccentric Contractions.
المؤلفون: Deyhle MR, Tiede D; Department of Kinesiology, Indiana University-Purdue University Indianapolis, Indianapolis IN., Xin L; Exercise Science Program, Biology Department, Simmons University, Boston, MA., Hyldahl RD; Department of Exercise Sciences, Brigham Young University, Provo UT., Hubal MJ; Department of Kinesiology, Indiana University-Purdue University Indianapolis, Indianapolis IN.
المصدر: Medicine and science in sports and exercise [Med Sci Sports Exerc] 2024 Jun 01; Vol. 56 (6), pp. 1108-1117. Date of Electronic Publication: 2024 Jan 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 8005433 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-0315 (Electronic) Linking ISSN: 01959131 NLM ISO Abbreviation: Med Sci Sports Exerc Subsets: MEDLINE
أسماء مطبوعة: Publication: Hagerstown, Md : Lippincott Williams & Wilkins
Original Publication: Madison, Wis., American College of Sports Medicine.
مواضيع طبية MeSH: Myalgia*/genetics , Creatine Kinase*/blood , Biomarkers*/blood , Muscle Strength*, Humans ; Male ; Young Adult ; Quadriceps Muscle/metabolism ; Adult ; Muscle Contraction/physiology ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/injuries ; Exercise/physiology ; Gene Expression
مستخلص: Purpose: Unaccustomed eccentric (ECC) exercise evokes exercise-induced muscle damage (EIMD). Soreness, strength loss, and serum creatine kinase (CK) are often used to quantify EIMD severity. However, changes in these markers are not fully understood mechanistically. To test the hypothesis that muscle damage markers are associated with unique molecular processes, we correlated gene expression responses with variation in each marker post-ECC.
Methods: Vastus lateralis biopsies were collected from 35 young men 3 h post-ECC (10 sets of 10 maximal eccentric contractions; contralateral leg [CON] as control). Maximal isometric strength, soreness, and serum CK activity were assessed 24 h preexercise and every 24 h for 5 d post-ECC. Strength was also measured 10 min post-ECC. Over the 5 d after ECC, average peak strength loss was 51.5 ± 20%; average soreness increased from 0.9 ± 1.9 on a 100-mm visual analog scale to 39 ± 19; serum CK increased from 160 ± 130 to 1168 ± 3430 U·L -1 . Muscle RNA was used to generate gene expression profiles. Partek Genomics Suite correlated peak values of soreness, strength loss, and CK post-ECC with gene expression in ECC (relative to paired CON) using Pearson linear correlation ( P < 0.05) and repeated-measures ANOVA used to detect influence of ECC.
Results: After ECC, 2677 genes correlated with peak soreness, 3333 genes with peak strength loss, and 3077 genes with peak CK. Less than 1% overlap existed across all markers (16/9087). Unique genes included 2346 genes for peak soreness, 3032 genes for peak strength loss, and 2937 genes for peak CK.
Conclusions: The largely unique molecular pathways associated with common indirect markers of EIMD indicate that each marker of "damage" represents unique mechanistic processes.
(Copyright © 2024 by the American College of Sports Medicine.)
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تواريخ الأحداث: Date Created: 20240131 Date Completed: 20240515 Latest Revision: 20240515
رمز التحديث: 20240515
DOI: 10.1249/MSS.0000000000003399
PMID: 38294822
قاعدة البيانات: MEDLINE
الوصف
تدمد:1530-0315
DOI:10.1249/MSS.0000000000003399