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Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content.

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العنوان: Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content.
المؤلفون: Hur GH; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Lee TK; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea., Cho YJ; Bio-MAX Institute, Seoul National University, Seoul, Republic of Korea., Kim JH; Department of Food Science and Biotechnology, Sungshin University, Seoul, Republic of Korea.; Basic Science Research Institute, Sungshin University, Seoul, Republic of Korea., Park JHY; Bio-MAX Institute, Seoul National University, Seoul, Republic of Korea., Yang H; Department of Food and Nutrition, Kookmin University, Seoul, Republic of Korea., Lee KW; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.; Bio-MAX Institute, Seoul National University, Seoul, Republic of Korea.; Advanced Institute of Convergence Technology, Seoul National University, Suwon, Republic of Korea.; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.; Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang, Republic of Korea.; Center for Food and Bio convergence, Seoul National University, Seoul, Republic of Korea.
المصدر: Journal of the science of food and agriculture [J Sci Food Agric] 2024 Jul; Vol. 104 (9), pp. 5010-5020. Date of Electronic Publication: 2024 Feb 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: England NLM ID: 0376334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0010 (Electronic) Linking ISSN: 00225142 NLM ISO Abbreviation: J Sci Food Agric Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005-> : Chichester, West Sussex : John Wiley & Sons
Original Publication: London, Society of Chemical Industry.
مواضيع طبية MeSH: Raphanus*/chemistry , Raphanus*/growth & development , Raphanus*/metabolism , Germination*/drug effects , Plant Extracts*/chemistry , Plant Extracts*/isolation & purification , Plant Extracts*/pharmacology , Isothiocyanates*/pharmacology , Isothiocyanates*/isolation & purification , Isothiocyanates*/chemistry , Isothiocyanates*/analysis, Mice ; Animals ; 3T3-L1 Cells ; Sulfoxides
مستخلص: Background: Cruciferous vegetable sprout has been highlighted as a promising functional material rich in bioactive compounds called isothiocyanates (ITCs) and it can be grown in very short periods in controlled indoor farms. However, because ITCs content depends on multiple factors such as cultivar, germination time and myrosinase activity, those variables need to be controlled during germination or extraction to produce functional materials enriched in ITCs. Sulforaphene (SFEN), an ITC found primarily in radishes (Raphanus sativus L.), exerts beneficial effects on obesity. However, the optimal germination and extraction conditions for radish sprout (RSP) to increase SFEN content remain unascertained, and the extract's anti-obesity effect has yet to be evaluated.
Results: The present study found that the SFEN content was highest in purple radish sprout (PRSP) among the six cultivars investigated. Optimal SFEN content occurred after 2 days of PRSP germination (2 days PRSP). To maximize the dry matter yield, total ITCs and SFEN contents in RSP extract, we found the optimal conditions for extracting PRSP [27.5 °C, 60 min, 1:75.52 solute/solvent (w/v), no ascorbic acid] using response surface methodology. Consistent with high SFEN content, 2 days PRSP extract significantly outperformed 3 days or 4 days PRSP extract in inhibiting lipid accumulation in 3T3-L1 cells. Moreover, 2 days PRSP extract suppressed adipogenesis and lipogenesis-related protein expression.
Conclusion: Regarding the cultivar, germination time and extraction conditions, optimally produced PRSP extract contains high SFEN content and exerts anti-obesity effects. Thus, we suggest PRSP extract as a potent functional material for obesity prevention. © 2024 Society of Chemical Industry.
(© 2024 Society of Chemical Industry.)
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معلومات مُعتمدة: BK21 Plus Program of the Department of Agricultural Biotechnology from Seoul National University; Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Technology Commercialization Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (821027)
فهرسة مساهمة: Keywords: functional materials; obesity; purple radish sprout; sulforaphene; total isothiocyanates
المشرفين على المادة: 0 (Plant Extracts)
0 (Isothiocyanates)
NCO9MC39IO (sulphoraphene)
0 (Sulfoxides)
تواريخ الأحداث: Date Created: 20240205 Date Completed: 20240614 Latest Revision: 20240614
رمز التحديث: 20240614
DOI: 10.1002/jsfa.13357
PMID: 38314949
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-0010
DOI:10.1002/jsfa.13357