دورية أكاديمية
Methods for the optimal preservation of blow fly intra-puparial forms for morphological analysis in forensic casework.
| العنوان: | Methods for the optimal preservation of blow fly intra-puparial forms for morphological analysis in forensic casework. |
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| المؤلفون: | López-García J; Universidad de Alcalá, Alcalá de Henares, Spain. jaime.lopezg@edu.uah.es., Piña MA; Universidad Nacional Autónoma de México, Ciudad de Mexico, Mexico., Clark B; Natural History Museum, London, UK., Hall MJR; Natural History Museum, London, UK., Martín-Vega D; Universidad de Alcalá, Alcalá de Henares, Spain.; Natural History Museum, London, UK. |
| المصدر: | International journal of legal medicine [Int J Legal Med] 2024 Jul; Vol. 138 (4), pp. 1769-1779. Date of Electronic Publication: 2024 Feb 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 9101456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1437-1596 (Electronic) Linking ISSN: 09379827 NLM ISO Abbreviation: Int J Legal Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Heidelberg, FRG : Springer International, c1990- |
| مواضيع طبية MeSH: | Forensic Entomology* , Calliphoridae*/growth & development , Pupa* , Specimen Handling*/methods , Postmortem Changes* , Ethanol*, Animals ; Immersion ; Preservation, Biological/methods ; Hot Temperature |
| مستخلص: | Accurate minimum post-mortem interval (minPMI) estimations often rely on a precise age determination of insect developmental stages, which is significantly influenced by environmental temperature. An optimal preservation of the entomological samples collected at crime scenes is pivotal for a reliable aging of immature insect samples. For blow flies (Diptera: Calliphoridae), the most widely used insect indicators in forensic investigations, an appropriate preservation of tissues is particularly important in the case of puparial samples because aging methods for intra-puparial forms usually depend on morphological analyses; however, although informative soft tissues and structures could be discoloured and/or distorted if they are not properly fixed, there is a lack of studies to assess different methods for the optimal preservation of intra-puparial forms collected in forensic investigations. The present study compares three preservation methods for intra-puparial forms of the blow fly Calliphora vicina Robineau-Desvoidy, 1830: (i) direct immersion into 80% ethanol, (ii) puncturing of the puparium and hot water killing (HWK) prior to preservation in 80% ethanol, and (iii) HWK without puncturing before preservation in 80% ethanol. External and internal morphological analyses of intra-puparial forms of different ages were conducted to assess the quality of preservation. The results indicate that direct immersion in ethanol led to poor preservation, affecting both external and internal tissues. Both methods with HWK resulted in a better preservation, but puncturing resulted, in some cases, in physical damage of the specimens. HWK without puncturing emerged as the optimal preservation method, consistently yielding high preservation scores for both external and internal morphological analyses. These findings have practical implications for forensic practitioners and emphasise the need for updating some published guidelines and protocols in forensic entomology. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Forensic entomology; Forensic science; Puparium; Sampling protocol; Storage; minPMI |
| المشرفين على المادة: | 3K9958V90M (Ethanol) |
| تواريخ الأحداث: | Date Created: 20240207 Date Completed: 20240610 Latest Revision: 20240613 |
| رمز التحديث: | 20240613 |
| مُعرف محوري في PubMed: | PMC11164728 |
| DOI: | 10.1007/s00414-024-03172-9 |
| PMID: | 38326653 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1437-1596 |
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| DOI: | 10.1007/s00414-024-03172-9 |