دورية أكاديمية
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Revealing the genome of the microsporidian Vairimorpha bombi, a potential driver of bumble bee declines in North America.

التفاصيل البيبلوغرافية
العنوان: Revealing the genome of the microsporidian Vairimorpha bombi, a potential driver of bumble bee declines in North America.
المؤلفون: Webster VL; Department of Biological Sciences, Royal Holloway University of London, London TW20 0EX, UK., Hemmings S; MRC Centre for Global Infectious Disease Analysis, Imperial College London, London W2 1PG, UK., Pérez M; Department of Biological Sciences, Royal Holloway University of London, London TW20 0EX, UK., Fisher MC; MRC Centre for Global Infectious Disease Analysis, Imperial College London, London W2 1PG, UK., Brown MJF; Department of Biological Sciences, Royal Holloway University of London, London TW20 0EX, UK., Farrer RA; MRC Centre for Medical Mycology, University of Exeter, Exeter EX4 4QD, UK.
المصدر: G3 (Bethesda, Md.) [G3 (Bethesda)] 2024 Apr 03; Vol. 14 (4).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Print Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Bethesda, MD : Genetics Society of America, 2011-
مواضيع طبية MeSH: Ecosystem* , Nosema*/genetics , Microsporidia*, Bees/genetics ; Animals ; Phylogeny ; North America
مستخلص: Pollinators are vital for food security and the maintenance of terrestrial ecosystems. Bumblebees are important pollinators across northern temperate, arctic, and alpine ecosystems, yet are in decline across the globe. Vairimorpha bombi is a parasite belonging to the fungal class Microsporidia that has been implicated in the rapid decline of bumblebees in North America, where it may be an emerging infectious disease. To investigate the evolutionary basis of pathogenicity of V. bombi, we sequenced and assembled its genome using Oxford Nanopore and Illumina technologies and performed phylogenetic and genomic evolutionary analyses. The genome assembly for V. bombi is 4.73 Mb, from which we predicted 1,870 protein-coding genes and 179 tRNA genes. The genome assembly has low repetitive content and low GC content. V. bombi's genome assembly is the smallest of the Vairimorpha and closely related Nosema genera, but larger than those found in the Encephalitozoon and Ordospora sister clades. Orthology and phylogenetic analysis revealed 18 core conserved single-copy microsporidian genes including the histone acetyltransferase (HAT) GCN5. Surprisingly, V. bombi was unique to the microsporidia in not encoding the second predicted HAT ESA1. The V. bombi genome assembly annotation included 265 unique genes (i.e. not predicted in other microsporidia genome assemblies), 20% of which encode a secretion signal, which is a significant enrichment. Intriguingly, of the 36 microsporidian genomes we analyzed, 26 also had a significant enrichment of secreted signals encoded by unique genes, ranging from 6 to 71% of those predicted genes. These results suggest that microsporidia are under selection to generate and purge diverse and unique genes encoding secreted proteins, potentially contributing to or facilitating infection of their diverse hosts. Furthermore, V. bombi has 5/7 conserved spore wall proteins (SWPs) with its closest relative V. ceranae (that primarily infects honeybees), while also uniquely encoding four additional SWPs. This gene class is thought to be essential for infection, providing both environmental protection and recognition and uptake into the host cell. Together, our results show that SWPs and unique genes encoding a secretion signal are rapidly evolving in the microsporidia, suggesting that they underpin key pathobiological traits including host specificity and pathogenicity.
Competing Interests: Conflicts of interest. The author(s) declare no conflicts of interest.
(© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America.)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; MR/V033417/1 United Kingdom MRC_ Medical Research Council; MR/N006364/2 United Kingdom MRC_ Medical Research Council; 225303/Z/22/Z United Kingdom WT_ Wellcome Trust; MR/MR/R015600/1 United Kingdom MRC_ Medical Research Council
فهرسة مساهمة: Keywords: Vairimorpha bombi; genome assembly; histone acetyltransferase; microsporidia; secretome; spore wall proteins
سلسلة جزيئية: figshare 10.6084/m9.figshare.24354313
SCR Organism: Vairimorpha cheracis
تواريخ الأحداث: Date Created: 20240209 Date Completed: 20240404 Latest Revision: 20240520
رمز التحديث: 20240521
مُعرف محوري في PubMed: PMC10989860
DOI: 10.1093/g3journal/jkae029
PMID: 38334143
قاعدة البيانات: MEDLINE
الوصف
تدمد:2160-1836
DOI:10.1093/g3journal/jkae029