دورية أكاديمية
Flipping the script: Understanding riboswitches from an alternative perspective.
| العنوان: | Flipping the script: Understanding riboswitches from an alternative perspective. |
|---|---|
| المؤلفون: | Olenginski LT; Department of Biochemistry, University of Colorado, Boulder, Colorado, USA., Spradlin SF; Department of Biochemistry, University of Colorado, Boulder, Colorado, USA., Batey RT; Department of Biochemistry, University of Colorado, Boulder, Colorado, USA. Electronic address: Robert.Batey@colorado.edu. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2024 Mar; Vol. 300 (3), pp. 105730. Date of Electronic Publication: 2024 Feb 08. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Ligands* , Riboswitch*/genetics , RNA, Messenger*/chemistry , RNA, Messenger*/genetics , RNA, Messenger*/metabolism, Bacteria/genetics ; Bacteria/metabolism ; Aptamers, Nucleotide/chemistry ; Aptamers, Nucleotide/genetics ; Aptamers, Nucleotide/metabolism ; Gene Expression Regulation, Bacterial |
| مستخلص: | Riboswitches are broadly distributed regulatory elements most frequently found in the 5'-leader sequence of bacterial mRNAs that regulate gene expression in response to the binding of a small molecule effector. The occupancy status of the ligand-binding aptamer domain manipulates downstream information in the message that instructs the expression machinery. Currently, there are over 55 validated riboswitch classes, where each class is defined based on the identity of the ligand it binds and/or sequence and structure conservation patterns within the aptamer domain. This classification reflects an "aptamer-centric" perspective that dominates our understanding of riboswitches. In this review, we propose a conceptual framework that groups riboswitches based on the mechanism by which RNA manipulates information directly instructing the expression machinery. This scheme does not replace the established aptamer domain-based classification of riboswitches but rather serves to facilitate hypothesis-driven investigation of riboswitch regulatory mechanisms. Based on current bioinformatic, structural, and biochemical studies of a broad spectrum of riboswitches, we propose three major mechanistic groups: (1) "direct occlusion", (2) "interdomain docking", and (3) "strand exchange". We discuss the defining features of each group, present representative examples of riboswitches from each group, and illustrate how these RNAs couple small molecule binding to gene regulation. While mechanistic studies of the occlusion and docking groups have yielded compelling models for how these riboswitches function, much less is known about strand exchange processes. To conclude, we outline the limitations of our mechanism-based conceptual framework and discuss how critical information within riboswitch expression platforms can inform gene regulation. Competing Interests: Conflict of interest R. T. B. serves on the Scientific Advisory Boards of Expansion Therapeutics, SomaLogic and MeiraGTx. All other authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | R01 GM073850 United States GM NIGMS NIH HHS; R35 GM152029 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: RNA folding; RNA structure; gene regulation; riboswitch; strand exchange |
| المشرفين على المادة: | 0 (Ligands) 0 (Riboswitch) 0 (Aptamers, Nucleotide) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20240209 Date Completed: 20240403 Latest Revision: 20240920 |
| رمز التحديث: | 20240920 |
| مُعرف محوري في PubMed: | PMC10907184 |
| DOI: | 10.1016/j.jbc.2024.105730 |
| PMID: | 38336293 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1083-351X |
|---|---|
| DOI: | 10.1016/j.jbc.2024.105730 |