دورية أكاديمية
أعرض في EDS

Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.

التفاصيل البيبلوغرافية
العنوان: Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.
المؤلفون: Bell EL; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Rosetto G; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA., Ingraham MA; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Ramirez KJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Lincoln C; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Clarke RW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Gado JE; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Lilly JL; Battelle Memorial Institute, Columbus, OH, 43201, USA., Kucharzyk KH; Battelle Memorial Institute, Columbus, OH, 43201, USA., Erickson E; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Beckham GT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA. gregg.beckham@nrel.gov.; BOTTLE Consortium, Golden, CO, 80401, USA. gregg.beckham@nrel.gov.
المصدر: Nature communications [Nat Commun] 2024 Feb 09; Vol. 15 (1), pp. 1217. Date of Electronic Publication: 2024 Feb 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Nylons* , Polymers*/chemistry, Caprolactam/*analogs & derivatives, Hydrolysis ; Polyesters
مستخلص: Successes in biocatalytic polyester recycling have raised the possibility of deconstructing alternative polymers enzymatically, with polyamide (PA) being a logical target due to the array of amide-cleaving enzymes present in nature. Here, we screen 40 potential natural and engineered nylon-hydrolyzing enzymes (nylonases), using mass spectrometry to quantify eight compounds resulting from enzymatic nylon-6 (PA6) hydrolysis. Comparative time-course reactions incubated at 40-70 °C showcase enzyme-dependent variations in product distributions and extent of PA6 film depolymerization, with significant nylon deconstruction activity appearing rare. The most active nylonase, a NylC K variant we rationally thermostabilized (an N-terminal nucleophile (Ntn) hydrolase, NylC K -TS, T m  = 87.4 °C, 16.4 °C higher than the wild-type), hydrolyzes 0.67 wt% of a PA6 film. Reactions fail to restart after fresh enzyme addition, indicating that substrate-based limitations, such as restricted enzyme access to hydrolysable bonds, prohibit more extensive deconstruction. Overall, this study expands our understanding of nylonase activity distribution, indicates that Ntn hydrolases may have the greatest potential for further development, and identifies key targets for progressing PA6 enzymatic depolymerization, including improving enzyme activity, product selectivity, and enhancing polymer accessibility.
(© 2024. The Author(s).)
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المشرفين على المادة: 25038-54-4 (nylon 6)
0 (Nylons)
0 (Polymers)
0 (Polyesters)
6879X594Z8 (Caprolactam)
تواريخ الأحداث: Date Created: 20240209 Date Completed: 20240214 Latest Revision: 20240214
رمز التحديث: 20240214
مُعرف محوري في PubMed: PMC10858056
DOI: 10.1038/s41467-024-45523-5
PMID: 38336849
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-45523-5