دورية أكاديمية
Quantification of multiple respiratory viruses in wastewater in the Kathmandu Valley, Nepal: Potential implications of wastewater-based epidemiology for community disease surveillance in developing countries.
| العنوان: | Quantification of multiple respiratory viruses in wastewater in the Kathmandu Valley, Nepal: Potential implications of wastewater-based epidemiology for community disease surveillance in developing countries. |
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| المؤلفون: | Raya S; Department of Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan., Malla B; Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan., Shrestha S; Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan., Sthapit N; Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan., Kattel H; Department of Microbiology, Tribhuvan University Teaching Hospital, Kathmandu, Nepal., Sharma ST; Department of Microbiology, Tribhuvan University Teaching Hospital, Kathmandu, Nepal., Tuladhar R; Department of Microbiology, Tribhuvan University, Kathmandu, Nepal., Maharjan R; Department of Civil Engineering, Institute of Engineering, Tribhuvan University, Lalitpur, Nepal., Takeda T; Department of Earth and Planetary Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: tomokotakeda@eps.s.u-tokyo.ac.jp., Kitajima M; Division of Environmental Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan. Electronic address: mkitajima@eng.hokudai.ac.jp., Tandukar S; Organization for Public Health and Environment, Lalitpur, Nepal., Haramoto E; Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan. Electronic address: eharamoto@yamanashi.ac.jp. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Apr 10; Vol. 920, pp. 170845. Date of Electronic Publication: 2024 Feb 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Wastewater-Based Epidemiological Monitoring* , Influenza A virus*, Nepal/epidemiology ; Wastewater ; Developing Countries ; RNA, Viral ; SARS-CoV-2 |
| مستخلص: | Despite being the major cause of death, clinical surveillance of respiratory viruses at the community level is very passive, especially in developing countries. This study focused on the surveillance of three respiratory viruses [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IFV-A), and respiratory syncytial virus (RSV)] in the Kathmandu Valley, Nepal, by implication of wastewater-based epidemiology (WBE). Fifty-one untreated wastewater samples were from two wastewater treatment plants (WWTPs) between April and October 2022. Among eight combinations of the pre-evaluated methods, the combination of concentration by simple centrifugation, pretreatment by DNA/RNA Shield (Zymo Research), and extraction by the QIAamp Viral RNA Mini Kit (QIAGEN) showed the best performance for detecting respiratory viruses. Using this method with a one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR), SARS-CoV-2 RNA was successfully detected from both WWTPs (positive ratio, 100 % and 81 %) at concentrations of 5.6 ± 0.6 log Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Eiji Haramoto received research funding from Takara Bio Inc. The other authors declared that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Influenza A virus; Nepal; RT-qPCR; Respiratory syncytial virus; SARS-CoV-2; Wastewater-based epidemiology |
| المشرفين على المادة: | 0 (Wastewater) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20240210 Date Completed: 20240306 Latest Revision: 20240306 |
| رمز التحديث: | 20240306 |
| DOI: | 10.1016/j.scitotenv.2024.170845 |
| PMID: | 38340866 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2024.170845 |