دورية أكاديمية
Phosphatidylcholine-deficient suppressor mutant of Sinorhizobium meliloti, altered in fatty acid synthesis, partially recovers nodulation ability in symbiosis with alfalfa (Medicago sativa).
العنوان: | Phosphatidylcholine-deficient suppressor mutant of Sinorhizobium meliloti, altered in fatty acid synthesis, partially recovers nodulation ability in symbiosis with alfalfa (Medicago sativa). |
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المؤلفون: | García-Ledesma JD; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Cuernavaca, Morelos, CP 62210, Mexico., Cárdenas-Torres L; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos, CP 62210, Mexico., Martínez-Aguilar L; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Cuernavaca, Morelos, CP 62210, Mexico., Chávez-Martínez AI; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos, CP 62210, Mexico., Lozano L; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Cuernavaca, Morelos, CP 62210, Mexico., López-Lara IM; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Cuernavaca, Morelos, CP 62210, Mexico., Geiger O; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad s/n, Cuernavaca, Morelos, CP 62210, Mexico. |
المصدر: | The Plant journal : for cell and molecular biology [Plant J] 2024 May; Vol. 118 (4), pp. 1136-1154. Date of Electronic Publication: 2024 Feb 11. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Oxford : Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology, c1991- |
مواضيع طبية MeSH: | Sinorhizobium meliloti*/physiology , Sinorhizobium meliloti*/genetics , Medicago sativa*/microbiology , Medicago sativa*/genetics , Plant Root Nodulation*/genetics , Symbiosis* , Fatty Acids*/metabolism , Fatty Acids*/biosynthesis , Phosphatidylcholines*/metabolism , Phosphatidylcholines*/biosynthesis, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Root Nodules, Plant/microbiology ; Root Nodules, Plant/genetics ; Root Nodules, Plant/metabolism ; Mutation ; Polysaccharides, Bacterial/metabolism ; Polysaccharides, Bacterial/biosynthesis ; Nitrogen Fixation |
مستخلص: | Rhizobial phosphatidylcholine (PC) is thought to be a critical phospholipid for the symbiotic relationship between rhizobia and legume host plants. A PC-deficient mutant of Sinorhizobium meliloti overproduces succinoglycan, is unable to swim, and lacks the ability to form nodules on alfalfa (Medicago sativa) host roots. Suppressor mutants had been obtained which did not overproduce succinoglycan and regained the ability to swim. Previously, we showed that point mutations leading to altered ExoS proteins can reverse the succinoglycan and swimming phenotypes of a PC-deficient mutant. Here, we report that other point mutations leading to altered ExoS, ChvI, FabA, or RpoH1 proteins also revert the succinoglycan and swimming phenotypes of PC-deficient mutants. Notably, the suppressor mutants also restore the ability to form nodule organs on alfalfa roots. However, nodules generated by these suppressor mutants express only low levels of an early nodulin, do not induce leghemoglobin transcript accumulation, thus remain white, and are unable to fix nitrogen. Among these suppressor mutants, we detected a reduced function mutant of the 3-hydoxydecanoyl-acyl carrier protein dehydratase FabA that produces reduced amounts of unsaturated and increased amounts of shorter chain fatty acids. This alteration of fatty acid composition probably affects lipid packing thereby partially compensating for the previous loss of PC and contributing to the restoration of membrane homeostasis. (© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.) |
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معلومات مُعتمدة: | P20 RR16475 United States RR NCRR NIH HHS; P20 RR16475 United States RR NCRR NIH HHS |
فهرسة مساهمة: | Keywords: 3‐hydroxydecanoyl‐acyl carrier protein‐dehydratase; FabA; Nod factor; RpoH1; phosphatidylcholine; phosphatidylethanolamine; sigma factor; succinoglycan; swimming ability |
تواريخ الأحداث: | Date Created: 20240211 Date Completed: 20240514 Latest Revision: 20240514 |
رمز التحديث: | 20240515 |
DOI: | 10.1111/tpj.16661 |
PMID: | 38341846 |
قاعدة البيانات: | MEDLINE |
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