دورية أكاديمية
In vitro and in planta investigation of succinate dehydrogenase inhibitor resistance conferred by target-site amino acid substitutions in Puccinia horiana, chrysanthemum white rust.
| العنوان: | In vitro and in planta investigation of succinate dehydrogenase inhibitor resistance conferred by target-site amino acid substitutions in Puccinia horiana, chrysanthemum white rust. |
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| المؤلفون: | Matsuzaki Y; Health and Crop Sciences Research Laboratory, Sumitomo Chemical Co., Ltd., Takarazuka, Japan., Harada T; Health and Crop Sciences Research Laboratory, Sumitomo Chemical Co., Ltd., Takarazuka, Japan., Iwahashi F; Health and Crop Sciences Research Laboratory, Sumitomo Chemical Co., Ltd., Takarazuka, Japan. |
| المصدر: | Pest management science [Pest Manag Sci] 2024 Jun; Vol. 80 (6), pp. 2874-2880. Date of Electronic Publication: 2024 Feb 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
| مواضيع طبية MeSH: | Succinate Dehydrogenase*/genetics , Succinate Dehydrogenase*/antagonists & inhibitors , Fungicides, Industrial*/pharmacology , Drug Resistance, Fungal*/genetics , Amino Acid Substitution* , Puccinia*, Plant Diseases/microbiology ; Chrysanthemum/microbiology ; Fungal Proteins/genetics ; Basidiomycota/physiology ; Basidiomycota/genetics |
| مستخلص: | Background: Resistance to succinate dehydrogenase inhibitor (SDHI) fungicides has been reported in some rust fungi within Pucciniales. However, measuring the resistance factors conferred by a specific substitution at the target site is difficult for most species because of the difficulty in performing in vitro experiments and the complexity of the binuclear state in these obligate parasites. We focused on Puccinia horiana because it easily forms homozygous basidiospores that are sensitive to SDHIs during in vitro germination, whereas the uredospores of other rust fungi are less sensitive. Results: We identified two substitutions, SdhC-I88F and SdhD-C125Y, that drive SDHI resistance in Pu. horiana. Using basidiospore germination inhibition tests, we measured the resistance factors for six SDHI fungicides in Pu. horiana isolates harboring SdhC-I88F substitutions, wherein orthologous substitutions were most frequently observed in SDHI-resistant Pucciniales, such as soybean rust (Phakopsora pachyrhizi). The resistance factors were high for penthiopyrad and benzovindiflupyr (>150), moderate for oxycarboxin and inpyrfluxam (10-30), and low for mepronil and fluxapyroxad (3-10). The most potent SDHI against SdhC-I88F-harboring isolates was inpyrfluxam, with a half-maximal effective concentration (EC Conclusion: This study is the first to use basidiospore germination inhibitory tests to quantify the resistance factors for SDHI-resistant Pucciniales. Owing to its homozygous binucleate nature and the high availability of basidiospores, Pu. horiana is useful for investigating SDHI resistance in Pucciniales. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. (© 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.) |
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| فهرسة مساهمة: | Keywords: Puccinia horiana; Pucciniales; fungicide resistance; resistance factor; succinate dehydrogenase inhibitor |
| المشرفين على المادة: | EC 1.3.99.1 (Succinate Dehydrogenase) 0 (Fungicides, Industrial) 0 (Fungal Proteins) |
| تواريخ الأحداث: | Date Created: 20240212 Date Completed: 20240513 Latest Revision: 20240513 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1002/ps.7995 |
| PMID: | 38345375 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1526-4998 |
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| DOI: | 10.1002/ps.7995 |