دورية أكاديمية
Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production.
| العنوان: | Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production. |
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| المؤلفون: | Huang M; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China.; College of Life Science and Technology, Hubei Engineering University, Xiaogan, P.R. China., Cui X; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China., Zhang P; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China., Jin Z; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China., Li H; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China., Liu J; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China., Jiang Z; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, P.R. China. |
| المصدر: | Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2024 Sep; Vol. 54 (8), pp. 1058-1067. Date of Electronic Publication: 2024 Feb 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9607037 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2297 (Electronic) Linking ISSN: 10826068 NLM ISO Abbreviation: Prep Biochem Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005-> : Abingdon, Oxford : Taylor & Francis Original Publication: New York, NY : Marcel Dekker, c1996- |
| مواضيع طبية MeSH: | Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Xylose*/metabolism , Ethanol*/metabolism , Glucose*/metabolism , Fermentation* , Metabolic Engineering*/methods , Aldose-Ketose Isomerases*/genetics , Aldose-Ketose Isomerases*/metabolism, Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Monosaccharide Transport Proteins/genetics ; Monosaccharide Transport Proteins/metabolism |
| مستخلص: | Saccharomyces cerevisiae cannot assimilate xylose, second to glucose derived from lignocellulosic biomass. Here, the engineered S. cerevisiae strains INV Sc -XI and INV Sc -XI/XT were constructed using xylA and Xltr1p to co-utilize xylose and glucose, achieving economic viability and sustainable production of fuels. The xylose utilization rate of INV Sc -XI/XT was 2.3-fold higher than that of INV Sc -XI, indicating that overexpressing Xltr1p could further enhance xylose utilization. In mixed sugar media, a small amount of glucose enhanced the consumption of xylose by INV Sc -XI/XT. Transcriptome analysis showed that glucose increased the upregulation of acetate of coenzyme A synthetase ( ACS ), alcohol dehydrogenase ( ADH ), and transketolase (TKL) gene expression in INV Sc -XI/XT, further promoting xylose utilization and ethanol yield. The highest ethanol titer of 2.91 g/L with a yield of 0.29 g/g at 96 h by INV Sc -XI/XT was 56.9% and 63.0% of the theoretical ethanol yield from glucose and xylose, respectively. These results showed overexpression of xylA and Xltr1p is a promising strategy for improving xylose and glucose conversion to ethanol. Although the ability of strain INV Sc -XI/XT to produce ethanol was not very satisfactory, glucose was discovered to influence xylose utilization in strain INV Sc -XI/XT. Altering the glucose concentration is a promising strategy to improve the xylose and glucose co-utilization. |
| فهرسة مساهمة: | Keywords: Saccharomyces cerevisiae; transcriptome analysis; xylose and glucose fermentation; xylose isomerase; xylose transporter Local Abstract: [plain-language-summary] INV Sc -XI and INV Sc -XI/XT strains were newly constructed to utilize xylose and glucose. XylA , in combination with xylose transporter Xltr1p , enhances xylose consumption.A small amount of glucose enhanced xylose utilization in INV Sc -XI/XT strain.The expression of ACS, ADH, and TKL genes is upregulated in the media containing mixed sugars.The highest ethanol yield of 0.29 g/g was produced in a 2-L scale-up fermenter. |
| المشرفين على المادة: | A1TA934AKO (Xylose) 3K9958V90M (Ethanol) IY9XDZ35W2 (Glucose) EC 5.3.1.5 (xylose isomerase) EC 5.3.1.- (Aldose-Ketose Isomerases) 0 (Saccharomyces cerevisiae Proteins) 0 (Monosaccharide Transport Proteins) |
| تواريخ الأحداث: | Date Created: 20240213 Date Completed: 20240902 Latest Revision: 20240909 |
| رمز التحديث: | 20240910 |
| DOI: | 10.1080/10826068.2024.2315479 |
| PMID: | 38349751 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-2297 |
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| DOI: | 10.1080/10826068.2024.2315479 |