دورية أكاديمية
أعرض في EDS

Homogeneous Polyporus polysaccharide exerts anti-bladder cancer effects via autophagy induction.

التفاصيل البيبلوغرافية
العنوان: Homogeneous Polyporus polysaccharide exerts anti-bladder cancer effects via autophagy induction.
المؤلفون: Luo S; The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China., Huang X; The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China., Li S; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China., Chen Y; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China., Zhang X; The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China., Zeng X; The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.; State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.
المصدر: Pharmaceutical biology [Pharm Biol] 2024 Dec; Vol. 62 (1), pp. 214-221. Date of Electronic Publication: 2024 Feb 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: [London] : Taylor & Francis
Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998-
مواضيع طبية MeSH: Polyporus*/metabolism , Urinary Bladder Neoplasms*/drug therapy, Humans ; Proto-Oncogene Proteins c-akt/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Apoptosis ; TOR Serine-Threonine Kinases/metabolism ; Autophagy ; Cell Proliferation ; Polysaccharides/pharmacology ; Autophagy-Related Proteins/pharmacology
مستخلص: Context: Polyporus polysaccharide (PPS), the leading bioactive ingredient extracted from Polyporus umbellatus (Pers.) Fr. (Polyporaceae), has been demonstrated to exert anti-bladder cancer and immunomodulatory functions in macrophages.
Objective: To explore the effects of homogeneous Polyporus polysaccharide (HPP) on the proliferation and autophagy of bladder cancer cells co-cultured with macrophages.
Materials and Methods: MB49 bladder cancer cells and RAW264.7 macrophages were co-cultured with or without HPP intervention (50, 100, or 200 μg/mL) for 24 h. The cell counting kit-8 (CCK-8) assay and 5-ethynyl-2″-deoxyuridine (EdU) staining evaluated MB49 cell proliferation. Monodansylcadaverine (MDC) staining and transmission electron microscopy (TEM) observed autophagosomes. Western blotting detected the expression levels of autophagy-related proteins and PI3K/Akt/mTOR pathway proteins.
Results: HPP inhibited the proliferation of MB49 cells co-cultured with RAW264.7 cells but not MB49 cells alone. HPP altered the expression of autophagy-related proteins and promoted the formation of autophagosomes in MB49 cells in the co-culture system. Autophagy inhibitors 3-methyladenine (3-MA) and chloroquine (CQ) not only antagonized HPP-induced autophagy but also attenuated the inhibitory effects of HPP on MB49 cell proliferation in the co-culture system. HPP or RAW264.7 alone was not sufficient to induce autophagy in MB49 cells. In addition, HPP suppressed the protein expression of the PI3K/Akt/mTOR pathway in MB49 cells in the co-culture system.
Discussion and Conclusions: HPP induced bladder cancer cell autophagy by regulating macrophages in the co-culture system, resulting in the inhibition of cancer cell proliferation. The PI3K/Akt/mTOR pathway was involved in HPP-induced autophagy in the co-culture system.
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فهرسة مساهمة: Keywords: Bladder cancer; PI3K/Akt/mTOR pathway
المشرفين على المادة: EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
EC 2.7.11.1 (TOR Serine-Threonine Kinases)
0 (Polysaccharides)
0 (Autophagy-Related Proteins)
تواريخ الأحداث: Date Created: 20240214 Date Completed: 20240215 Latest Revision: 20240217
رمز التحديث: 20240217
مُعرف محوري في PubMed: PMC10868468
DOI: 10.1080/13880209.2024.2316195
PMID: 38353262
قاعدة البيانات: MEDLINE
الوصف
تدمد:1744-5116
DOI:10.1080/13880209.2024.2316195